ZHAO Xiaoqiong,LIANG Taishuai,ZHANG Henghui.Effects of Chitooligosaccharide on Growth and Quality of Brassica chinensis L. Seedlings Under NaCl Stress[J].Northern Horticulture,2020,44(15):32-37.[doi:10.11937/bfyy.20194521]
壳寡糖对NaCl胁迫下盆栽小白菜生长和品质的影响
- Title:
- Effects of Chitooligosaccharide on Growth and Quality of Brassica chinensis L. Seedlings Under NaCl Stress
- Keywords:
- Brassica chinensis L.; chitooligosaccharide; NaCl stress; growth physiology; photosynthesis; quality
- 文献标志码:
- A
- 摘要:
- 以“七宝青”小白菜为试材，采用温室盆栽方法，探究叶面喷施100 mg?L-1壳寡糖（COS）溶液对不同浓度盐胁迫（土壤NaCl含量0.3%、0.6%和1.2%）下小白菜生长、生理特性和品质的影响。结果表明：相比于0.3%和0.6% NaCl胁迫，100 mg?L-1 COS溶液对1.2% NaCl胁迫的缓解效果更好，可明显增强小白菜植株的抗氧化能力，缓解NaCl胁迫对小白菜生长、光合作用和品质的抑制，主要表现为小白菜株高、主根长、地上部和根部干质量显著增加，盐害指数下降；净光合速率(Pn)、气孔导度（Gs）和蒸腾速率（Tr）显著增加，胞间CO2浓度（Ci）显著下降；叶绿素a、叶绿素a+b和类胡萝卜素含量显著增加；超氧阴离子（O?[TX-*4]2）、丙二醛（MDA）含量显著下降，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性显著增加；可溶性蛋白质、可溶性糖、维生素C和游离氨基酸含量显著增加，硝酸盐含量显著降低。
- Abstract:
- Taking ‘Qibaoqing’ Brassica chinensis L.as experimental materials，a greenhouse potting experiment was performed to explore the effects of foliar-spraying 100 mg?L-1 chitooligosaccharide solution (COS) on the growth，physiological characteristics and quality of Brassica chinensis L.seedlings under different levels of salt stress (0.3%，0.6% and 1.2% of soil NaCl contents).The results showed that compared with 0.3% NaCl and 0.6% NaCl stress，100 mg?L-1 COS solution had a better alleviation effect on 1.2% NaCl stress，which could significantly enhance the antioxidant capacity and alleviate the inhibition of NaCl stress on growth，photosynthesis and quality of Brassica chinensis L.seedlings，with the plant height，root length，shoot and root dry weight，Pn，Gs，Tr，contents of chlorophyll a，chlorophyll a+b and carotenoid，activities of SOD，POD and CAT，and contents of soluble protein，soluble sugar，vitamin C and free amino acid increased obviously，and with the salt injury index，Ci，contents of O?[TX-*4]2，MDA and nitrate decreased significantly.
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备注/Memo
第一作者简介：赵肖琼（1988-），女，山西汾阳人，硕士，讲师，现主要从事植物逆境生理及分子机制等研究工作。E-mail:xiaoqiong_zhao@126.com.基金项目：山西省应用基础研究计划资助项目（201801D221279）。收稿日期：2019-12-01