XU Xuefen,NI Chunhui,LI Huixia,et al.Identification of Codonopsis pilosula Powdery Mildew New Fungi andGermination Characteristics of Conidia[J].Northern Horticulture,2020,44(15):111-117.[doi:10.11937/bfyy.20194043]
党参白粉病新病原鉴定及其分生孢子的萌发特性
- Title:
- Identification of Codonopsis pilosula Powdery Mildew New Fungi andGermination Characteristics of Conidia
- 关键词:
- 党参白粉病; rDNA-ITS序列; 28S D1/D2基因; 小蓬草单囊白粉菌; 分生孢子萌发
- Keywords:
- powdery mildew on Codonopsis pilosula; rDNA-ITS sequence; 28S D1/D2 gene; P.erigerntis-canadensis; conidia germination
- 文献标志码:
- A
- 摘要:
- 以甘肃省陇西县采集的党参白粉病病叶为试材，采用形态学特征观察及rDNA-ITS序列、28S D1/D2基因测序，对其病原菌进行种类鉴定，并采用悬滴法进行孢子萌发试验。以期明确该地区党参白粉病的病原菌种类及其分生孢子的萌发特性并为病害防治提供参考依据。结果表明：该病原菌菌丝粗2～8 μm，附着胞乳头形；闭囊壳近球形，附属丝5～17根，多生于子囊果一侧，长23～131 μm，为子囊果直径的0.19～1.51倍；内含一个子囊，子囊眼直径4～15 μm，子囊孢子8个。该菌分生孢子萌发最佳温度为25 ℃，最适宜pH为6，参与试验的4种碳源对孢子萌发无影响，持续光照40 h对分生孢子的萌发有较大的抑制作用。形态特征与小蓬草单囊白粉菌（Podosphaera erigerntis-canadensis）一致。经MEGA 6.0软件分析，其rDNA-ITS序列、28S D1/D2基因均与P.erigerntis-canadensis聚在一起，与登录号为KJ660809和AB462764的序列同源性分别为99.62%和99.82%，经检索党参病害，由P.erigerntis-canadensis引起的党参白粉病国内尚鲜见报道。
- Abstract:
- Taking the powdery mildew leaves of Codonopsis pilosula collected in Longxi County of Gansu Province as test materials, the pathogen species were identified by morphological observation, rDNA-ITS sequence and 28S D1/D2 gene sequencing, and spore germination test was conducted by suspension drop method. In order to clarified the pathogen species and conidia germination characteristics of Codonopsis pilosula in this area, and provided reference for disease control. The results showed that the mycelium of the pathogen was 2－8 μm thick and had papillae appressorium; the ascocarps were approximately spherical with 5－17 accessory filaments, most of which were on one side of the ascocarp and were 23－131 μm long, 0.19－1.51 times of diameter of the ascocarps; it contained one ascus with eye diameters of 4－15 μm and 8 ascospores per ascus. The optimum temperature for conidia germination was 25 ℃, and the optimum pH was 6. The tested four carbon sources had no effect on conidia germination. Continuous light (more than 40 hours) had great inhibition on conidia germination. All the morphological characteristics were identified with Podosphaera erigerntis-canadensis. The rDNA-ITS sequence and 28S D1/D2 gene were clustered with P.erigerntis-canadensis by MEGA 6.0 software analysis. The similarities with KJ660809 and AB462764 were 99.62% and 99.82% respectively. The powdery mildew of Codonopsis pilosula caused by P.erigerntis-canadensis had not been reported in China.
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备注/Memo
第一作者简介：徐雪芬（1995-），女，硕士研究生，研究方向为植物病原真菌学。E-mail:1599429649@qq.com.责任作者：李惠霞（1972-），女，博士，教授，博士生导师,现主要从事植物线虫及真菌学等研究工作。E-mail:lihx@gsau.edu.cn.基金项目：国家重点研发计划资助项目（2018YFC1706301）;甘肃省中药材产业技术体系资助项目。收稿日期：2020-01-15