ZHANG Jiachen,JIA Fuchen,LUO Zhang,et al.Identification Research of Seven Wild Macrofungus Based on ITS Sequences[J].Northern Horticulture,2020,44(08):122-129.[doi:10.11937/bfyy.20193803]
基于ITS序列对林芝市售七种野生大型食用真菌的鉴定
- Title:
- Identification Research of Seven Wild Macrofungus Based on ITS Sequences
- 文献标志码:
- A
- 摘要:
- 以西藏林芝市售7种野生大型真菌（分别编号为XZQGJ、XZDJG、NCSBJ、NCNGJ、NCTLJ、NCSR、NCWZNGJ）为试材，采用分子生物学的方法对其进行鉴定,为进一步开发利用林芝地区大型真菌资源提供了参考依据。结果表明：XZQGJ属于腊伞属的Hygrophorus russula（红菇腊伞）；XZDJG属于肉齿菌的Sarcodon cf.leucopus（裂盖肉齿菌）；NCSBJ属于枝瑚菌属，与Ramaria sp.（小刺枝瑚菌）（KY774253.1）亲缘性最近，但林芝扫把菌与其它相似种均有一定的遗传距离，因此林芝扫把菌是一个独立的种；NCNGJ属于牛肝菌属，与Boletus recapitulatus(KP938967.1)亲缘性最近；NCTLJ属于钉菇属，与Gomphus clavatus（棒状钉菇）(KX008988.1)亲缘性最近；NCSR属于口蘑属，与Tricholoma matsutake(松口蘑)（MF521899.1）亲缘性最近，但林芝松茸与其它相似种均有一定的遗传距离，因此林芝松茸是一个独立的种；NCWZNGJ属于牛肝菌科的uncultured Boletaceae（未驯化牛肝菌）。
- Abstract:
- Seven types of wild macrofungi(numbered XZQGJ，XZDJG，NCSBJ，NCNGJ，NCTLJ，NCSR，NCWZNGJ) from Nyingchi in Tibet were used as test materials,which were identified by morphology. The results showed that XZQGJ belonged to Hygrophorus russula;XZDJG belonged to Sarcodon cf.leucopus;NCSBJ belonged to Mycobacterium，and Ramaria sp.(KY774253.1) had the closest affinity，but NCSBJ had a certain genetic distance from other similar species，so NCSBJ was an independent species;NCNGJ belonged to to the genus Boletus and was closest to Boletus recapitulatus (KP938967.1);NCTLJ belonged to the genus Flammulina and was closest to Gomphus clavatus(KX008988.1);NCSR belonged to Tricholoma and was related to Tricholoma matsutake (MF521899.1).Lingzhi Matsutake had a certain genetic distance from other similar species，so NCSR was an independent species;NCWZNGJ belonged to the uncultured Boletaceae.The results showed a theoretical basis for further development and their application value of wild macrofungi resources in Nyingchi.
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备注/Memo
第一作者简介：张家晨（1996-），男，硕士研究生，研究方向为食品微生物。E-mail:648189793@qq.com.责任作者：薛蓓（1983-），女，硕士，副教授，研究方向为食品微生物及天然产物。E-mail:xuebei_1128@126.com.基金项目：食品科学与工程重点学科建设资助项目（502218009）；西藏中央支持地方高校发展专项资助项目（503118004）；西藏自治区特色农产品加工贮藏团队资助项目（2018KYTD-02）。收稿日期：2020-01-10