WANG Yue,YIN Jie,WANG Ran,et al.Construction of High Frequently Regeneration System From Leaves of Dwarfing Rootstock ‘Qingzhen D3’ of Pear[J].Northern Horticulture,2020,44(09):17-22.[doi:10.11937/bfyy.20193750]
梨矮化砧木‘青砧D3’叶片高效再生体系的构建
- Title:
- Construction of High Frequently Regeneration System From Leaves of Dwarfing Rootstock ‘Qingzhen D3’ of Pear
- Keywords:
- pear; ‘Qingzhen D3’; leaves regeneration; tissue culture
- 文献标志码:
- A
- 摘要:
- 以梨矮化砧木‘青砧D3’为试材，进行了离体叶片组织培养高效再生体系的构建。结果表明：TDZ和IBA均可以促进‘青砧D3’离体叶片不定芽的诱导，但TDZ效果更显著，其诱导效果和TDZ的浓度成正比，研究发现‘青砧D3’离体叶片不定芽再生最佳培养基为NN69+TDZ 3.0 mg?L-1+IBA 0.3 mg?L-1+琼脂7 g?L-1+蔗糖30 g?L-1，再生率与再生芽数分别为100.00%和7.69；IBA对‘青砧D3’不定根的诱导优于NAA，不定根再生最佳培养基为1/2MS+IBA 0.2 mg?L-1+琼脂7 g?L-1+蔗糖30 g?L-1，根诱导率达到100%。该研究的开展为梨矮化砧木‘青砧D3’的工厂化育苗奠定了基础。
- Abstract:
- High frequently regeneration system of leaves in vitro was established by using dwarfing rootstock ‘Qingzhen D3’ of pear as materials.The results showed that adventitious shoots of leaves in vitro could be induced by TDZ and IBA,and TDZ′s induction effect,which was proportional to its concentration,was better than that of IBA.The optimal culture medium of shoots regeneration was NN69+TDZ 3.0 mg?L-1+IBA 0.3 mg?L-1+agar 7 g?L-1+sugar 30 g?L-1,and 100.00% regeneration rate and 7.69 buds per leaf was achieved.And the results also revealed that adventitious roots could be induced by IBA and NAA,and induction effect of IBA was better than that of NAA.The optimal culture medium of roots induction was 1/2MS supplemented with IBA 0.2 mg?L-1,agar 7 g?L-1,and sugar 30 g?L-1,and 100% regeneration rate was achieved.This study laid a foundation for industrialization of seedling production of ‘Qingzhen D3’.
参考文献/References:
[1]ROMERO P,NAVARRO J M,PREZ-PREZ J,et al.Deficit irrigation and rootstock:Their effects on water relations,vegetative development,yield,fruit quality and mineral nutrition of Clemenules mandarin[J].Tree Physiology,2006,26(12):1537-1548.[2]WEBSTER A D.A brief review of pear rootstock development[J].Acta Hortic,1998,475:135-142.[3]邓秀新,束怀瑞,郝玉金,等.果树学科百年发展回顾[J].农学学报,2018(1):28-34.[4]姜淑苓,欧春青,王斐,等.我国梨矮化砧木的发展与应用[J].北方果树,2018(2):1-3.[5]王震星,张磊,刘玉芹,等.杜梨绿枝扦插快速繁殖的研究[J].天津农业科学,2000,6(4):13-15.[6]屈柏林,曹墨盈,崔硕,等.杜梨砧木粗度和高度对短截压条萌蘖数量的影响[J].安徽农学通报,2015(8):66-67.[7]蒋海月,习洋,张军.12种常用耐盐植物的嫩枝扦插比较研究[J].河北林果研究,2010(1):19-21.[8]薛明超,马春晖,李鼎立,等.不同梨砧木嫩枝扦插繁殖技术研究[J].中国果树,2015(3):35-38.[9]李秀根,杨健,王龙,等.我国近30a梨育种研究进展与今后工作建议[J].果树学报,2010,27(6):987-994.[10]姜敏,蒲富慎,贾敬贤,等.“榅桲+哈代”砧嫁接中国梨的生育表现[J].中国果树,1987(4):25-29.[11]徐明义,姚芳玲.梨属矮化砧木研究[J].西北农业学报,1997,6(1):69-73.[12]姜淑苓,贾敬贤,纪宝生,等.梨矮化砧木-中矮1号[J].中国果树,2000(3):1-3.[13]姜淑苓,陈长兰,贾敬贤,等.梨矮化砧木新品种‘中矮2号’[J].园艺学报,2006,43(6):11.[14]李登科,邵嘉鸣,张忠仁,等.梨K系矮化自根砧木的选育[J].中国果树,1997(3):20-21.[15]王际轩,隋长琴.苹果砧木组织培养繁殖的研究[J].中国农业科学,1981,14(6):33-37.[16]李晓青,张晓申,王慧瑜.甜樱桃矮化砧木吉塞拉的组织培养和快速繁殖[J].北方园艺,2008(7):226-227.[17]潘增光,邓秀新.苹果组织培养再生技术研究进展[J].果树学报,1998(3):261-266.[18]汤绍虎,孙敏,周启贵,等.‘雪青’梨叶片高频再生体系的建立[J].园艺学报,2005,32(6):1084-1087.[19]王文成,马春晖,王然,等.杜梨不定根形成相关基因PbARRO-1的克隆及定量表达分析[J].北方园艺,2017(12):97-102.[20]YANG Y,WANG D,WANG C,et al.Construction of high efficiency regeneration and transformation systems of Pyrus ussuriensis Maxim[J].Plant Cell,Tissue and Organ Culture (PCTOC),2017,131(1):139-150.[21]周佳红,邢才华,曹慧,等.秋子梨茎段薄层细胞培养再生初探[J].北方园艺,2015(6):95-98.[22]YOUSEFIARA M,JAFARKHANI K M,BAGHERI A,et al.Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.)[J].Journal of Plant Molecular Breeding,2014,2(1):21-28.[23]NITSCH J P,NITSCH C.Haploid plants from pollen grains[J].Science,1969,163(3862):85-87.
相似文献/References:
[1]侯玉华,孙 正,张玉星.河北省果品梨流通体系的现状、问题及对策[J].北方园艺,2013,37(22):201.
HOU Yu-hua,SUN Zheng,ZHANG Yu-xing.Study on the Current Situation,Problems and Countermeasures of the Pear Circulation in Hebei Province[J].Northern Horticulture,2013,37(09):201.
[2]冯娟娟,刘军弟,霍学喜.世界主要落叶水果主产国生产与贸易预测分析[J].北方园艺,2014,38(04):195.
[3]王尚堃,杜红阳.“晚秋黄”梨省力化高效丰产栽培技术[J].北方园艺,2014,38(06):59.
[J].Northern Horticulture,2014,38(09):59.
[4]张晓峰,韩翔,张建光.不同土壤水分含量对黄冠梨叶片光合效率和抗氧化特性的影响[J].北方园艺,2013,37(10):7.
ZHANG Xiao-feng,HAN Xiang,ZHANG Jian-guang.Effect of Different Soil Moisture Contents on Net Photosynthetic Rate and Antioxidant Abilities in Huangguan Pear Leaves[J].Northern Horticulture,2013,37(09):7.
[5]贾春霞,张小珍,马春晖.梨园春季晚霜冻害发生与预防技术[J].北方园艺,2013,37(11):197.
JIA Chun-xia,ZHANG Xiao-zhen,MA Chun-hui.Causes and Preventing Techniques of Frost Injury in the Pear Orchard[J].Northern Horticulture,2013,37(09):197.
[6]刘珠琴,舒巧云,蒋国强,等.梨人工辅助授粉时间、花朵序位及方法研究[J].北方园艺,2012,36(01):35.
LIU Zhu-qin,SHU Qiao-yun,JIANG Guo-qiang,et al.Study on the Pear Assisted Pollination Time, Flowers Ordinal and Method[J].Northern Horticulture,2012,36(09):35.
[7]李金龙,马光恕.不同储藏方式对梨果实硬度及PPO活性的影响[J].北方园艺,2013,37(09):164.
LI Jin-long,MA Guang-shu.Effect of Different Storage Methods on the Fruit Firmness and PPO Activity of Pear[J].Northern Horticulture,2013,37(09):164.
[8]董晓勤,周 鹏,仇宗浩,等.两个品种绿皮梨及其红色芽变品种光合生理特性的比较[J].北方园艺,2013,37(20):1.
DONG Xiao-qin,ZHOU Peng,QIU Zong-hao,et al.Comparison of Photosynthetic Physiological Characteristics of Two Varieties of Green Pears and Their Red Mutations Varieties[J].Northern Horticulture,2013,37(09):1.
[9]徐凌飞,李致慧,贾东峰,等.梨矮化砧木云南榅桲离体快繁研究[J].北方园艺,2012,36(05):113.
XU Ling-fei,LI Zhi-hui,JIA Dong-feng,et al.Study on in vitro Propagation of Yunnan Quince as a Dwarf Rootstock of Pear[J].Northern Horticulture,2012,36(09):113.
[10]王俊英,李富军.1-甲基环丙烯对“丰水梨”冷藏品质的影响[J].北方园艺,2012,36(18):165.
Influence of -MCP on Hosui Pear Storage Quality.[J].Northern Horticulture,2012,36(09):165.
备注/Memo
第一作者简介：王月（1995-），女，硕士研究生，研究方向为果树分子生物学。E-mail：lhqxxwy@163.com.责任作者：李鼎立（1979-），男，博士，副教授，研究方向为果树分子生物学。E-mail：qauldl@163.com.基金项目：国家自然科学基金青年基金资助项目（31401832）；现代农业产业技术体系建设专项资金资助项目（CARS-29）；国家重点研发计划资助项目（2018YFD0201400）。收稿日期：2020-01-11