TIAN Huai,HOU Na,GONG Minggui.Optimization of Extraction and Ntioxidation of Polyphenols in Houttuynia cordata Thunb. by Response Surface Methodology[J].Northern Horticulture,2020,44(17):94-101.[doi:10.11937/bfyy.20193659]
响应面试验优化鱼腥草中多酚提取及抗氧化性
- Title:
- Optimization of Extraction and Ntioxidation of Polyphenols in Houttuynia cordata Thunb. by Response Surface Methodology
- Keywords:
- response surface method; Houttuynia cordata; polyphenols; antioxidant
- 文献标志码:
- A
- 摘要:
- 以贵州野生的鱼腥草为试材，采用响应面法优化鱼腥草多酚分离的工艺条件，以得到的多酚含量作为响应值，乙醇体积分数、提取时间、料液比、提取温度作为提取单因素，以期构建响应面确定最佳工艺条件，并且通过对?OH和O?[TX-*4]2的清除作用来评估鱼腥草多酚抗氧化能力。结果表明：鱼腥草中多酚的最佳提取条件为料液比1∶30 g?mL-1，超声萃取温度为45 ℃，乙醇浓度为55%，时间为40 min，在最佳条件下的鱼腥草多酚获得最大值为3.57%；以提取率作为响应值得到的回归方程预测模型结果与单因素试验吻合，提取时间和料液比对多酚提取贡献突出，该研究为鱼腥草多酚的深层发掘与提高利用率提供参考依据。
- Abstract:
- The wild Houttuynia cordata Thunb.in Guizhou was used as experimental material,using the response surface method optimization of Houttuynia cordata polyphenols separation process conditions,to get the content of polyphenols as response value,volumetric fraction of ethanol,extraction time,solid-liquid ratio,extraction temperature as the extraction of single factor constructing the response surface to determine the best process conditions,through the (?OH) and (O?[TX-*4]2) removal function to assess its polyphenols antioxidant capacity.The results showed that the optimum extraction conditions of polyphenols in Houttuynia cordata was material-liquid ratio was 1∶30 g?mL-1,ultrasonic extraction temperature 45 ℃,ethanol concentration 55%,the time for 40 minutes,under the best conditions of Houttuynia cordata polyphenols to obtain maximum value was 3.57%.On extraction yield as the regression equation of the response was worth to the prediction model coincides with the single factor experiment,extraction time and solid-liquid ratio on polyphenols extraction outstanding contribution,the research for deep excavation and improve the utilization rate of Houttuynia cordata polyphenols provide reference and basis.
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备注/Memo
第一作者简介：田怀（1967-），女，贵州贵阳人，本科，高级讲师，现主要从事植物资源开发等研究工作。E-mail:1648751687@qq.com.责任作者：侯娜（1983-），女，陕西户县人，博士，高级工程师，现主要从事林木遗传育种等研究工作。E-mail:849741705@qq.com.基金项目：贵州省科技厅中药现代化资助项目（黔科合ZY字[2012]3002号）；贵州省科技平台资助项目（黔科平台[2012]4002）。收稿日期：2019-09-25