[1]陈展,魏建国,赵艳卓,等.不同鲜食葡萄品种对霜霉病菌的抗性分析[J].北方园艺,2019,43(20):25-30.[doi:10.11937/bfyy.20190441]
　CHEN Zhan,WEI Jianguo,ZHAO Yanzhuo,et al.Analysis of Resistance to Downy Mildew in Table Grape Varieties[J].Northern Horticulture,2019,43(20):25-30.[doi:10.11937/bfyy.20190441]
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不同鲜食葡萄品种对霜霉病菌的抗性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年20 页码: 25-30 栏目: 出版日期: 2019-10-30
Title:
Analysis of Resistance to Downy Mildew in Table Grape Varieties
作者:
陈展; 魏建国; 赵艳卓; 牛帅科; 杨丽丽
（河北省农林科学院 石家庄果树研究所，河北 石家庄 050061）
Author(s):
CHEN Zhan; WEI Jianguo; ZHAO Yanzhuo; NIU Shuaike; YANG Lili
(Shijiazhuang Pomology Institute，Hebei Academy of Agriculture and Forestry，Shijiazhuang，Hebei 050061)
关键词:
霜霉病; 抗性鉴定; 半定量RT-PCR; 病程相关蛋白
Keywords:
downy mildew; resistance identification; semi-quantitative RT-PCR; pathogenesis-related proteins(PRs)
DOI:
10.11937/bfyy.20190441
文献标志码:
A
摘要:
以48个鲜食葡萄品种为试材，通过室内离体叶盘接种霜霉病菌试验，对各品种的霜霉病抗性分级，结合对抗病相关基因[STBX]PR1、PR5和NPR1[STBZ]的半定量RT-PCR试验，分析不同抗性级别品种在霜霉病菌侵染后表达模式的差异，以期为抗葡萄霜霉病的新品种选育提供参考依据。结果表明：48个品种中有30个品种对霜霉病高度敏感，7个品种对霜霉病敏感，4个品种中抗，6个品种高抗霜霉病，“金星无核”对霜霉病完全免疫。[STBX]PR1和PR5[STBZ]基因在接菌前有本底表达，抗病和高抗品种接菌后均上调表达，感病品种接菌后无明显变化，仅“无核翠宝”和“红地球”有微量下调。[STBX]NPR1[STBZ]基因接菌后在抗病品种中表达量上升，而在感病品种和高抗品种中均表现为半数品种上升，半数品种下降。
Abstract:
In order to provide reference for the breeding of downy mildew resistance varieties，the resistance to downy mildew of 48 table grape varieties were tested by leaf disc inoculation indoor.Meanwhile，the expression patterns of resistance-related genes [STBX]PR1，PR5 and NPR1[STBZ] to downy mildew were investigated through semi-quantitative RT-PCR.The results showed that 30 varieties were highly susceptible，7 susceptible，4 resistant，6 highly resistant，‘Venus Seedless’ immune to downy mildew.The difference of [STBX]PR1 and PR5[STBZ] expression were not significantly in susceptible varieties except ‘Wuhecuibao’ and ‘Red Globe’，which were reduced slightly after inoculation.The expression of [STBX]PR1 and PR5[STBZ] were higher in the resistant ones after downy mildew infection.In susceptible varieties and high-resistant varieties，the expression of gene [STBX]NPR1[STBZ] was up-regulated in 50% varieties and down-regulated in the other ones，while the expression was up-regulated in all the resistant varieties.

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备注/Memo

第一作者简介：陈展（1983-），女，硕士，助理研究员，研究方向为葡萄病虫害防控。E-mail:chenzhan_elle@126.com.责任作者：杨丽丽（1979-），女，博士，副研究员，现主要从事葡萄栽培和抗病品种选育等研究工作。E-mail:yanglili0311@163.com.基金项目：国家现代葡萄产业技术体系资助项目（CARS-29-3）;河北省农林科学院创新工程资助项目（F18R19-04）。收稿日期：2019-05-13

更新日期/Last Update: 2019-10-30