LI Ning,GAO Shenghua,WANG Fei,et al.Bio-informatics Analysis of the CONSTANS-like Transcription Factor Gene Family of Pepper[J].Northern Horticulture,2019,43(15):1-7.[doi:10.11937/bfyy.20184088]
辣椒CONSTANS-like转录因子家族的生物信息学分析
- Title:
- Bio-informatics Analysis of the CONSTANS-like Transcription Factor Gene Family of Pepper
- 关键词:
- 辣椒; CO-like基因家族; 生物信息学
- Keywords:
- pepper; CONSTANS-like gene family; bio-informatics
- 文献标志码:
- A
- 摘要:
- CONSTANS(CO)基因介于生物节律钟与下游开花基因之间,是光周期途径中的关键基因。以‘Zunla-1’辣椒基因组数据为试验材料,利用生物信息学工具对辣椒CO-like转录因子基因家族进行研究,以期为进一步揭示CO-like转录因子基因家族在辣椒开花中的作用提供参考。结果表明:‘Zunla-1’辣椒基因组中共有9个CO-like基因,CO-like的蛋白大小相似,理论等电点均小于7;多序列和进化树分析将辣椒CO-like基因家族划分为3个组;利用转录组数据对9个CO-like基因家族成员的基因表达情况进行了分析,发现上述基因在叶片均有表达。
- Abstract:
- CONSTANS (CO) gene is the key gene between biological rhythm clock and downstream flowering genes in photoperiod pathway.The CO-like transcription factor gene family of pepper was studied by means of bioinformatics using the genomic data of ‘Zunla-1’.In the study,the work provided a reference of the role of CO-like transcription factor gene family in pepper during flowering.The results showed that there were nine CO-like genes in ‘Zunla-1’ genome,the protein size of CO-like was similar,and the theoretical isoelectric point was less than 7.The CO-like gene family of capsicum was divided into three groups by multi-sequence and evolutionary tree analysis.The gene expression of nine members of CO-like gene family was analyzed by transcriptome data,and it was found that all the above genes were expressed in leaves.
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备注/Memo
第一作者简介:李宁(1982-),女,博士,副研究员,现主要从事辣椒遗传育种与生物技术等研究工作。E-mail:798986546@qq.com.责任作者:姚明华(1969-),男,博士,研究员,现主要从事辣椒遗传育种等研究工作。E-mail:yaomh_2008@126.com.基金项目:国家重点研发计划资助项目(2017YFD0101903,〖JP〗2016YFE0205500);现代农业产业技术体系资助项目(CARS-23-G-28);湖北省农业科学院人才资助项目(Q2018009,C2018003)。收稿日期:2019-02-25