TIAN Jinli,WANG Manman,WANG Yunli,et al.Detection of Bacterial Fruit Blotch in Seed-used Pumpkin and Seed Disinfection[J].Northern Horticulture,2019,43(22):60-64.[doi:10.11937/bfyy.20183829]
籽用南瓜细菌性果腐病种子带菌检测与消毒处理
- Title:
- Detection of Bacterial Fruit Blotch in Seed-used Pumpkin and Seed Disinfection
- 文献标志码:
- A
- 摘要:
- 以市场销售的5份籽用南瓜种子为试材，采用PCR检测技术对南瓜种子细菌性果腐病菌进行检测，用不同浓度的CuSO4、HCl、H2O2、过氧乙酸和漂白粉等药剂处理带菌种子进行消毒处理。研究了不同药剂处理对南瓜种子果腐病菌消毒效果的影响。结果表明：市场销售的5份南瓜种子均携带细菌性果腐病菌；5种药剂对南瓜种子携带的细菌性果腐病菌均有消毒效果，但HCl、H2O2、过氧乙酸对南瓜种子的发芽有抑制作用。1.0% CuSO4处理30 min和1.5%漂白粉处理30 min对南瓜种子果腐病菌防治效果最好，并且2种药剂处理对种子发芽率和发芽势影响不明显。因此，选用1.0% CuSO4或1.5%漂白粉对南瓜种子进行消毒处理，可以消除种子表皮所携带的果腐病病菌。
- Abstract:
- Seeds of (Cucurbita maxima) from market were detected for bacterial fruit blotch (BFB) by polymerase chain reaction (PCR) technology.To select the best treatment for seed disinfection,CuSO4,HCl,H2O2,peroxyacetic acid and bleaching powder with different concentrations treated the seeds-borne BFB.The effect of different agentia treatment on the disinfection of pumpkin seed rot pathogen was studied.The results showed that：BFB was detected in 5 different kind of seeds from market.In our study，all 5 treatments with different concentrations had bactericidal effect.However，HCL，H2O2 and peroxyacetic acid also had inhibitory effects on the germination of pumpkin seeds.1.0% CuSO4 with 30 minutes treatment and 1.5% bleaching powder with 30 minutes treatment achieved the best effect on BFB inhibition，and had no effect on seed germination.So，after 1.0% CuSO4 with 30 minutes treatment or 1.5% bleaching powder with 30 minutes treatment，BFB carried by pumpkin seeds could be effectively inhibited.
参考文献/References:
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备注/Memo
第一作者简介：田金丽（1992-），女，硕士研究生，研究方向为南瓜遗传育种。E-mail:772031877@qq.com. 责任作者：屈淑平（1973-），女，博士，教授，博士生导师，现主要从事南瓜分子遗传育种等研究工作。E-mail：spqu@neau.edu.cn.基金项目：公益性行业科研专项资助项目（201303112）。收稿日期：2019-03-27