YANG Yong,HAI Jun,PANG Keqin,et al.Effects of Drought on Seed Germination and Seedling Growth of Transgenic AtCuZnSOD Kale[J].Northern Horticulture,2019,43(12):76-82.[doi:10.11937/bfyy.20183680]
干旱对转AtCuZnSOD基因羽衣甘蓝种子萌发和幼苗生长的影响
- Title:
- Effects of Drought on Seed Germination and Seedling Growth of Transgenic AtCuZnSOD Kale
- 关键词:
- 羽衣甘蓝; AtCuZnSOD基因; 转基因植物; 耐旱性
- Keywords:
- kale; AtCuZnSOD gene; transgenic plant; drought tolerance
- 文献标志码:
- A
- 摘要:
- 以羽衣甘蓝名古屋（Brassica oleracea var.acephala f.tricolor）为试材，采用农杆菌介导、PCR分析、Southern blot和qRT-PCR等方法，研究了转拟南芥(Arabidopsis thaliana L.)铜/锌超氧化物歧化酶(AtCuZnSOD)基因的羽衣甘蓝的耐旱性，以期增加羽衣甘蓝抗性育种的种质资源。结果表明：AtCuZnSOD基因整合到羽衣甘蓝基因组中,转基因植物中AtCuZnSOD表达水平均明显增加。在PEG-4000胁迫下，转AtCuZnSOD基因羽衣甘蓝种子发芽势与发芽率均高于对照。在干旱胁迫下，转基因植株幼苗的丙二醛含量显著低于野生型植物，而过氧化物酶和过氧化氢酶活性均高于野生型植株；同时转基因植株的平均主根长、存活率和叶绿素含量的增加均显著高于野生植株，而干质量/鲜质量明显低于野生植物。表明转AtCuZnSOD基因羽衣甘蓝植株耐旱性得到明显改善。
- Abstract:
- Brassica oleracea was used as test material，drought tolerance of transformed kale with Arabidopsis thaliana L.copper/zinc superoxide dismutase (AtCuZnSOD) gene was studied by Agrobacterium-mediated，PCR，Southern blot and qRT-PCR，in order to increase the germplasm resources of resistance breeding in Kale.The results showed that AtCuZnSOD gene was integrated into the genome of kale.The expression level of AtCuZnSOD in transgenic plants increased significantly.Under PEG-4000 stress，the seed germination potential and germination rate of transgenic AtCuZnSOD kale were higher than those of control.Under drought stress，MDA content of transgenic seedlings was significantly lower than that of wild plants，while POD and CAT activities were higher than those of wild plants.Meanwhile，the increase of average main root length，survival rate and chlorophyll content of transgenic plants were significantly higher than that of wild plants，while dry weight/fresh weight was significantly lower than that of wild plants.The results showed that the drought tolerance of transgenic kale plants with AtCuZnSOD gene was significantly improved.
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备注/Memo
第一作者简介：杨勇（1982-）,男，河南驻马店人，硕士，工程师，现主要从事园林植物等研究工作。E-mail:461556594@qq.com.基金项目：河南省科技计划资助项目（182102110305）。收稿日期：2019-02-25