HU Xiaojing,TIAN Qing,GUAN Yuanjing,et al.Effect of Dolicin on Growth and Physiology of Phalaenopsis Seedlings[J].Northern Horticulture,2020,44(01):69-74.[doi:10.11937/bfyy.20183543]
喷施多利素对蝴蝶兰幼苗生长及生理的影响
- Title:
- Effect of Dolicin on Growth and Physiology of Phalaenopsis Seedlings
- Keywords:
- Phalaenopsis; dolicin; seedling; growth; physiology
- 文献标志码:
- A
- 摘要:
- 以蝴蝶兰品种‘兄弟女孩’组培苗为试材，采用叶面喷施的方式，研究了不同浓度的多利素肥料对蝴蝶兰幼苗生长状况和生理特性的影响，旨在筛选出最佳的施肥浓度，为其在蝴蝶兰上的合理施用提供参考依据。结果表明：多利素对蝴蝶兰的幼苗生长有促进作用，能有效促进蝴蝶兰的最大叶长、叶宽、叶面积、单叶质量、株鲜质量等增加；明显提高叶绿素含量（Chl）和可溶性蛋白质含量（SP）；增加超氧化物歧化酶（SOD）、过氧化物酶（POD）等活性；不同程度地影响净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）等光合参数。所测指标均值均优于对照，从高到低依次为T4＞T3＞T2＞T1＞对照。其中以500倍液的效果最好。该处理下蝴蝶兰的最大叶长、叶宽、叶面积分别较对照高出16.5%、7.2%、94.3%；单叶质量、株鲜质量也较对照增加43.4%、116.6%。同时，Chl、SP比对照增加71.9%、112.9%；SOD、POD活性较对照提高39.9%、47.2%。此外，各处理的Pn、Tr、Gs均值也高于对照，较对照增加122.5%、111.4%、108.3%；而Ci大小则随施肥浓度的升高而下降。综上，多利素可明显改善蝴蝶兰生长和营养状况，提高其酶活性和光合性能，以喷施500倍液的多利素效果最好，该浓度下所测指标的均值均显著高于对照。
- Abstract:
- Using tissue culture seedlings of Phalaenopsis variety ‘Brothers and Girls’ as material，the effects of different concentrations of dolicin fertilizer on the growth and physiological characteristics of Phalaenopsis seedlings were studied by foliar spraying，in order to select the best fertilizer concentration and provide a theoretical basis for its rational application on Phalaenopsis.The results showed that dolicin could obviously improve the growth,nutritional status，enzymatic activity and photosynthetic performance of Phalaenopsis，and the best effect was to spray dolicin 500 times solution.The average values of the indexes measured at this concentration were significantly higher than those of the control.Dolicin promoted the growth of Phalaenopsis seedlings，increased the maximum leaf length，leaf width，leaf area，single leaf weight and plant fresh weight，significantly increased chlorophyll content (Chl)，soluble protein content (SP)，superoxide dismutase (SOD)，peroxidase (POD) and so on.The photosynthetic parameters such as net photosynthetic rate (Pn)，transpiration rate (Tr) and stomatal conductance (Gs) were affected in different degrees.The mean values of the indexes measured were better than those of the control，and the order from high to low was treatment 4>treatment 3>treatment 2>treatment 1>control.In treatment 4，the maximum leaf length and width of Phalaenopsis were 16.5%,7.2% and 94.3% higher than that of the control，respectively，and the weight of single leaf and fresh weight of plant were 43.4% and 116.6% higher than that of the control.At the same time，the content of Chl and SP increased by 71.9% and 112.9% compared with the control.The activity of SOD and POD increased by 39.9% and 47.2% compared with the control.In addition，the average values of Pn，Tr and Gs of all treatments were higher than those of the control，which increased by 122.5%，111.4% and 108.3%，while Ci decreased with the increase of fertilizer concentration.The growth and nutritional status of Phalaenopsis could be significantly improved by doxycycline, and its enzyme activity and photosynthetic performance could be improved. The effect of doxycycline spraying 500 times solution was the best, and the mean value of the indexes measured under this concentration was significantly higher than that of the control.
参考文献/References:
[1]曾宋君,彭晓明,张京丽,等.蝴蝶兰的组织培养和快繁殖[J].武汉植物学研究,2000,18(4):344-346.[2]郭盛磊.供氮水平对落叶松幼苗光合作用的影响[J].生态学报,2005,25(6):1291-1298.[3]莎茹.基质类型与肥量水平对樟子松苗木影响的初步研究[D].呼和浩特:内蒙古农业大学,2013.[4]申超.樟子松轻基质营养包育苗技术的研究[D].呼和浩特:内蒙古农业大学,2013.[5]王建章.商品牡丹病虫害综合防治技术[J].河南农业,2012(11):34.[6]冯建,王俊.不同肥料配比对蝴蝶兰小苗生长的影响[J].农业科技通讯,2013(12):101-102.[7]许传怀.蝴蝶兰水肥管理技术[J].中国园艺文摘,2012,28(8):121-122.[8]陈菁瑛,蓝贺胜,陈雄鹰,等.不同栽培管理措施对蝴蝶兰生长的影响[J].林业科技开发,2000,16(1):29-30.[9]徐敏,刘君,阿衣古力?阿布都瓦依提.植物生理实验教学中叶绿素提取方法比较[J].实验科学与技术,2018,16(4):129-133.[10]赵英永,戴云,崔秀明,等.考马斯亮蓝G-250染色法测定草乌中可溶性蛋白质含量[J].云南民族大学学报(自然科学版),2006(3):235-237.[11]曲敏,秦丽楠,刘羽佳,等.两种检测SOD酶活性方法的比较[J].食品安全质量检测学报,2014,5(10):3318-3323.[12]李忠光,龚明.愈创木酚法测定植物过氧化物酶活性的改进[J].植物生理学通讯,2008(2):323-324.[13]丁雪,凌钧华,林雪粉,等.不同肥料对蝴蝶兰生长和开花品质的影响[J].亚热带植物科学,2017,46(2):188-191.[14]张沙艳,张勇.肥水浓度对蝴蝶兰长势的影响[J].辽宁师专学报,2006(1):103-105.[15]张明生,谢波,谈锋,等.甘薯可溶性蛋白、叶绿素及ATP含量变化与品种抗旱性关系的研究[J].中国农业科学,2003(1):13-16.[16]杨笑笑,左琪琪,杨磊,等.蝴蝶兰叶片叶绿素含量对栽培基质含水量和施肥量的响应[J].安徽农业科学,2015,43(27):48-50.[17]任国三,程加祥,王红对,等.茄子对低温胁迫的生理响应及不同品种耐冷性比较[J].中国蔬菜,2007(4):12-15.[18]高秀瑞,潘秀清,武彦荣,等.低温胁迫对茄子幼苗耐冷生理特性的影响[J].河北农业科学,2008,12(12):6-8.[19]韦泽秀.水肥对大棚黄瓜和番茄生理特性及土壤环境的影响[D].杨凌:西北农林科技大学,2009.[20]熊靓,龚伟,王景燕,等.配方施肥对汉源葡萄青椒叶片光合特性的影响[J].西北农林科技大学学报(自然科学版),2019(1):1-10..
相似文献/References:
[1]孙汝斌,余 纽,孙佩光.蝴蝶兰基因工程研究进展[J].北方园艺,2014,38(12):177.
SUN Ru-bin,YU Niu,SUN Pei-guang.Research Progress on Gene Engineering of Phalaenopsis Plants[J].Northern Horticulture,2014,38(01):177.
[2]董平,赵培宝,任爱芝.蝴蝶兰灰霉病菌的分离鉴定[J].北方园艺,2014,38(04):109.
DONG Ping,ZHAO Pei-bao,REN Ai-zhi.Isolation and Identification of Botrytis cinerea?in Phalaenopsis[J].Northern Horticulture,2014,38(01):109.
[3]王常芸,李晓亮,张京伟,等.蝴蝶兰组培快繁技术体系研究[J].北方园艺,2013,37(15):122.
WANG Chang-yun,LI Xiao-liang,ZHANG Jing-wei,et al.Study on Tissue Culture and Rapid Propagation System of Phalaenopsis[J].Northern Horticulture,2013,37(01):122.
[4]丁兰,郭艳,董刚,等.国产蝴蝶兰种苗携带建兰花叶病毒(CymMV)和齿兰环斑病毒 (ORSV)的调查及脱毒的初步研究[J].北方园艺,2012,36(02):137.
DING Lan,GUO Yan,DONG Gang,et al.Investigation and Preliminary Study of Elimination of CymMV and ORSV from Phalaenopsis in China[J].Northern Horticulture,2012,36(01):137.
[5]孔芳,凌宏龙,薛正莲,等.保鲜液对蝴蝶兰切花保鲜生理效应的研究[J].北方园艺,2013,37(02):130.
KONG Fang,LING Hong-long,XUE Zheng-lian,et al.Research on Preservatives of Cut Phalaenopsis Flower and Related Physiological Influence[J].Northern Horticulture,2013,37(01):130.
[6]景维杰,杨露露,蒋明殿.不同浓度氮、磷、钾对蝴蝶兰幼苗生长的影响[J].北方园艺,2012,36(23):62.
JING Wei-jie,YANG Lu-lu,JIANG Ming-dian.Effects of Different Concentrations of Nitrogen，Phosphorus and Potassium on the Growth of Phalaenopsis Seedlings[J].Northern Horticulture,2012,36(01):62.
[7]何荆洲,卜朝阳,黄昌艳,等.蝴蝶兰红花系品种“火凤凰”的杂交结实性研究[J].北方园艺,2015,39(02):61.[doi:10.11937/bfyy.201502017]
HE Jing-zhou,BU Zhao-yang,HUANG Chang-yan,et al.Study on Fecundity of Cross-pollination in Phalaenopsis ‘Fire Phoenix’[J].Northern Horticulture,2015,39(01):61.[doi:10.11937/bfyy.201502017]
[8]杜君,王利民,孟月娥,等.我国蝴蝶兰产业发展现状及对策研究[J].北方园艺,2015,39(09):172.[doi:10.11937/bfyy.201509047]
DU Jun,WANG Li-min,MENG Yue-e,et al.Study on the Development Status and Countermeasures of Phalaenopsis amabilis Industry in China[J].Northern Horticulture,2015,39(01):172.[doi:10.11937/bfyy.201509047]
[9]张 颖,郭 晓 东,王芳,等.蝴蝶兰组织培养快繁技术研究[J].北方园艺,2010,34(08):0.[doi:10.11937/bfyy.201008046]
ZHANG Ying,GUO Xiao -dong,W ANG Fang,et al.The Research on Tissue Culture and Rapid Propagation of Phalaenopsis Hybrid[J].Northern Horticulture,2010,34(01):0.[doi:10.11937/bfyy.201008046]
[10]王 桂 兰,陈 超,李 艳 梅,等.蝴蝶兰催花及开花过程中可溶性糖含量变化的研究[J].北方园艺,2010,34(10):0.[doi:10.11937/bfyy.2010010055]
W ANG Gui -lan,CHEN Chao,LI Yan -mei,et al.Study on Change of Soluble Sugar Content in Plant of Phalaenopsis During Flower Induction and Blooming Period[J].Northern Horticulture,2010,34(01):0.[doi:10.11937/bfyy.2010010055]
备注/Memo
第一作者简介：胡小京（1969-），女，贵州毕节人，硕士，副教授，现主要从事观赏植物栽培生理及生物技术等研究工作。E-mail:gdhxjyy@163.com.基金项目：贵阳市科技计划资助项目（筑科合同[2017]3-1号）。收稿日期：2019-05-22