LEI Kaijun,ZHENG Fanghao,LI Zihong,et al.Extraction of Volatile Oil From Mentha haplocalyx Based on Orthogonal Experimental Design[J].Northern Horticulture,2019,43(01):133-138.[doi:10.11937/bfyy.20182404]
正交实验设计优选薄荷芳香水的提取工艺
- Title:
- Extraction of Volatile Oil From Mentha haplocalyx Based on Orthogonal Experimental Design
- 文献标志码:
- A
- 摘要:
- 以薄荷为试材，建立气相色谱（GC）测定薄荷芳香水中薄荷脑含量的方法，色谱柱选择5%苯基-甲基聚硅氧烷为固定液的毛细管柱（30 m×0.32 mm×0.25 μm）；载气为氮气，流速1.5 mL·min-1，氢气流速40 mL·min-1；检测器温度280 ℃；进样口温度250 ℃；进样量为1 μL。采用水蒸气蒸馏法提取薄荷芳香水，以挥发油提取量和薄荷脑含量为考核指标，以加水量、药材浸泡时间和提取时间为考察因素，进行L9（34）正交实验，优选薄荷芳香水的提取工艺。结果表明：采用GC测定薄荷芳香水中薄荷脑的含量，薄荷脑浓度在0.02～0.20 mg·mL-1线性关系良好（r=0.999 8），准确度、精密度RSD均小于5%，色谱条件发生小的变动不会影响测定结果。正交实验结果表明，浸泡时间是影响薄荷挥发油提取的主要因素，其次为提取时间、加水量；最佳提取工艺为加8倍量水，浸泡1.5 h，提取3.0 h。
- Abstract:
- Taking Mentha haplocalyx as test material to study the extraction of volatile oil in Mentha haplocalyx by orthogonal experimental design.GC method was established to determine the contents of menthol.Gas chromatography (GC) was performed on the column of Agilent HP-5 capillary column (30 m×0.32 mm×0.25 μm).Nitrogen gas was used as carrier gas,and the flow rate was 1.5 mL·min-1.The flow rate of hydrogen was 40 mL·min-1.The detection temperature was 280 ℃.The injection temperature was 250 ℃.The injection volume was 1 μL.Volatile oil was extracted by steam distillation.Initial amount of solvent,soaking time,extraction time were examined by taking the volatile oil extraction amount and menthol content as the evaluation index.The preparation process was optimized by L9(34) orthogonal experimental design.The GC method was feasible and accurate for the determination of menthol in the volatile oil.The linear range was 0.02-0.20 mg·mL-1 (r=0.999 8),and RSDs of precision and accuracy tests were all lower than 5%.Soaking time was the key factor influencing the extraction of volatile oil.The optimal preparation for volatile oil were adding initial amount of water by 8 times,soaking for 1.5 hours and eatracting for 3.0 hours.
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备注/Memo
第一作者简介：雷凯君（1970-），女，本科，主任药师，现主要从事药事管理等研究工作。E-mail:huanglong1987924@163.com.责任作者：刘效仿（1964-），男，硕士，主任医师，现主要从事中西医结合治疗骨伤等研究工作。E-mail:530437860@qq.com.基金项目：广东省中医药局2015年度中医药强省专项资金-医院中药制剂开发资助项目；广东省中医药局面上资助项目（20171260，20181249）；2016年佛山市医学类科技攻关资助项目（2016AB002041）。收稿日期：2018-09-12