HUANG Jiao.The Antibacterial Activity of Volatile Oils and Water Extracts from Four Kinds of Acorus in Emei Mountain[J].Northern Horticulture,2014,38(21):67-69.
峨眉山四种菖蒲挥发油及水提取液的抑菌作用比较
- Title:
- The Antibacterial Activity of Volatile Oils and Water Extracts from Four Kinds of Acorus in Emei Mountain
- 文章编号:
- 1001-0009（2014）21-0067-03
- Keywords:
- Acorus; antibacterial effect; volatile oil; water extract
- 分类号:
- S 567.9
- 文献标志码:
- A
- 摘要:
- 采用纸片扩散法测定抑菌圈直径，试管二倍稀释法测定挥发油最低抑菌浓度（MIC），研究了峨眉山4种菖蒲挥发油及水提取液对3种常见致病菌的抑菌作用，以期为合理使用峨眉山菖蒲植物提供试验依据。结果表明：挥发油提取液对3种致病菌抑菌作用表现为茴香菖蒲>石菖蒲>水菖蒲，金钱菖蒲仅对大肠杆菌有弱抑菌作用。其中茴香菖蒲和石菖蒲挥发油对金黄色葡萄球菌有中等抑菌作用，最小抑菌浓度（MIC）最低，均为31.25 μg/mL，抑菌效果最强；茴香菖蒲、石菖蒲、水菖蒲对枯草芽孢杆菌和大肠杆菌为弱抑菌作用，最低抑菌浓度（MIC）各异。除石菖蒲水提取液对大肠杆菌有弱抑菌作用外，其余水提取液均无抑菌作用。
- Abstract:
- The disc diffusion method was adopted to measure the antibacterial circle diameter,and the tube dilution method was used to determinate the minimum inhibitory concentration(MIC),the antibacterial activity of volatile oils and water extracts from four kinds of Acorus in Emei mountain to three common pathogenic bacteria were reported，in order to provide the experimental basis for the rational use of the calamus plants in Emei mountain.The results showed that the antibaterial activity of volatile oils from A.macrospadiceus F.N.Wei&Y.K.Li was stronger than A.tatarinowii Schott.which was stronger than A.calamus L.to three pathogenic bacteria,while the volatile oils from A.gramineus Solander.to Escherichia coli was weak.The volatile oils from A.macrospadiceus F.N.Wei&Y.K.Li and A.tatarinowii Schott.to Staphyloccocus aureus were the strongest,had middle antibacterial effect and the MIC were both 31.25 μg/mL；the volatile oils from A.macrospadiceus F.N.Wei&Y.K.Li,A.tatarinowii Schott.,A.calamus L.to Bacillus subtilis and Escherichia coli had weak effect and the MIC were different.The water extrats had not antibaterial effect except the water extrats from A.tatarinowii Schott.had the weak effect to Escherichia coli.
参考文献/References:
[1]陈峰.菖蒲属植物的化学成分及药理作用[J].世界科学技术(中医药现代化),2011,13(6):1013-1017.
[2]黄远征,何宗英,曹延怀,等.中国菖蒲属植物根茎挥发油成分分析及其资源的合理利用[J].色谱,1993,11(5):267-270.
[3]Rai R,Gupta A,Siddiqui I R,et al.Xanthone glycoside from rhizome of Acorus calamus[J].Indian J Chem,Sect B:Org Chem Incl Med Chem,1999,38B(9):1143.
[4]陆顺忠,黎贵卿,李秋庭,等.茴香菖蒲挥发油化学成分的研究[J].广西农学报,2010,25(5):18-19,23.
[5]梁忠云,王国聪.草茴脑资源及生物活性应用研究进展[J].广西林业科学,2010,39(1):49-51.
[6]延静,谢令德,杨长举,等.水菖蒲提取物对4种储粮害虫的生物活性[J].华中农业大学学报,2006,25(5):515-517.
[7]何池全,陈少风,叶居新.石菖蒲抑菌效应的研究[J].环境与开发,1997,12(3):1-3,6.
[8]Deng Y C,Chen K L,Yu Y Z,et al.In vitro antifungal activity of the extract and compound from Acorus tatarinowii against seven plant pathogenic fungi[J].Agricultural Sciences in China,2010,9(1):71-76.
[9]范秀荣,李光武,沈萍.微生物学实验[M].北京:高等教育出版社,1996.
[10]赵兵,赵婷,高昂,等.3种秦艽药材提取物的抑菌作用比较[J].西北大学学报(自然科学版),2012,42(6):971-974.
[11]侯文久,杨凤影,王维新.中草药对刺参腐皮综合症病原茵的抑菌试验[J].中国农学通报,2013,29(5):76-80. [12]黄远征,左尧凤,何宗英,等.两种新的茴香脑资源植物[J].天然产物研究与开发,1991,3(3):18-24.
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备注/Memo
作者简介：黄娇（1981-），女，四川峨眉人，硕士，副教授，研究方向为植物资源及应用。E-mail：anyyke@sohu.com.
基金项目：四川省教育厅资助项目(12ZB241)；乐山师范学院校级课题资助项目(Z1323)。
收稿日期：2014-05-27