[1]王静,马腾斋,邱佳俊,等.NaCl胁迫对黑果枸杞幼苗生理及生化指标的影响[J].北方园艺,2019,43(06):59-64.[doi:10.11937/bfyy.20182325]
　WANG Jing,MA Tengzhai,QIU Jiajun,et al.Effects of NaCl Stress on Physiological and Biochemical Characteristics of Lycium ruthecium Murr.Seedling[J].Northern Horticulture,2019,43(06):59-64.[doi:10.11937/bfyy.20182325]
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NaCl胁迫对黑果枸杞幼苗生理及生化指标的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年06 页码: 59-64 栏目: 出版日期: 2019-03-30
Title:
Effects of NaCl Stress on Physiological and Biochemical Characteristics of Lycium ruthecium Murr.Seedling
作者:
王静; 马腾斋; 邱佳俊; 赵百慧; 闫兴富
（国家民委生态系统模型及应用重点试验室，北方民族大学 生物科学与工程学院，宁夏 银川 750021）
Author(s):
WANG Jing; MA Tengzhai; QIU Jiajun; ZHAO baihui; YAN Xingfu
(Key Laboratory of Ecosystem Model and Applications of State Nationalities Affairs Commission/College of Biological Science and Engineering,North Minzu University,Yinchuan,Ningxia 750021)
关键词:
NaCl胁迫; 黑果枸杞; 幼苗生长; 耐盐性
Keywords:
NaCl stress; Lycium ruthenicum Murr.; seedling growth; salt tolerance
DOI:
10.11937/bfyy.20182325
文献标志码:
A
摘要:
以黑果枸杞幼苗为试验材料，将幼苗在0、50、100、200 mmol?L-1 NaCl条件下处理10 d后，分析了主根伸长量、叶片花青素、游离脯氨酸、丙二醛、可溶性蛋白质含量、超氧化物歧化酶（SOD）活性和过氧化物酶（POD）活性及其同工酶等生理生化指标的变化，探究其适应盐生环境可能的机制，以期为合理开发利用黑果枸杞提供参考依据。结果表明：经0、50、100、200 mmol?L-1NaCl处理后，黑果枸杞幼苗主根生长未受抑制，0~100 mmol?L-1 NaCl处理时，叶片花青素含量变化不显著，200 mmol?L-1 NaCl处理后，花青素含量显著升高；0~100 mmol?L-1 NaCl处理时，幼苗中游离脯氨酸及丙二醛含量变化不显著，200 mmol?L-1 NaCl 处理后以上物质均显著升高；经0~200 mmol?L-1 NaCl处理，黑果枸杞幼苗中SOD活性及其同工酶酶谱表达稳定，变化不显著；POD活性先升后降，100 mmol?L-1 NaCl胁迫时活性最强，为1 065.9 U?g-1?min-1，且同工酶POD 1、POD 2与黑果枸杞幼苗响应盐胁迫紧密相关。
Abstract:
Lycium ruthenicum Murr.seedlings were treated with different medium contained 0,50,100 and 200 mmol?L-1 NaCl for 10 days,root length increasement,contents of anthocyanin in leaves,proline,MDA,soluble protein,activities and isozymes of POD and SOD response to salinity were analyzed.The effect of different concentration medium of NaCl on physiological and biochemical characteristics of Lycium ruthecium Murr.seedling was studied,which to detect mechanism of the plant adapt to salinity condition and provide scientific basis for future utilizing.The results showed that the increased root length was not influenced by 0-200 mmol?L-1NaCl stress medium.In 200 mmol?L-1 NaCl condition,anthocyanin in leaves was raised up and reached the highest level.The content of proline and MDA were not changed significantly under 0 to 100 mmol?L-1 NaCl stress,while all the characteristics elevated in response to 200 mmol?L-1 NaCl.With the increasing salinity,the activity and expression of SOD isozymes were stabilized.POD avtivity showed a trend of first rising and then falling along with the salt concentration increasing,the maximization activity was 1 065.9 U?g-1?min-1 in 100 mmol?L-1 NaCl medium.Isozymes of POD 1 and POD 2 were correlated with seedling salt tolerance.

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备注/Memo

第一作者简介：王静（1984-），女，博士，讲师，现主要从事植物逆境生物化学与分子生物学等研究工作。E-mail:wangjing_imu@163.com.基金项目：北方民族大学校级资助项目（2016skky08）；国家自然科学基金资助项目（31600202）。收稿日期：2018-10-08

更新日期/Last Update: 2019-04-18