PANG Hongyang,LI Hongli,LONG Zuoyi.Effects of IBA Treatment on Rooting Index and Related Oxidase Activities During Grow Seedlings With Root Tillers of Corylus heterophylla Fisch.× Corylus avellana L.[J].Northern Horticulture,2019,43(01):79-82.[doi:10.11937/bfyy.20181992]
IBA处理对杂交榛根蘖育苗生根指标及相关氧化酶活性的影响
- Title:
- Effects of IBA Treatment on Rooting Index and Related Oxidase Activities During Grow Seedlings With Root Tillers of Corylus heterophylla Fisch.× Corylus avellana L.
- Keywords:
- Corylus heterophylla Fisch.× Corylus avellana L.; IBA; grow seedlings with root tillers; oxidase
- 文献标志码:
- A
- 摘要:
- 以“龙榛1号”当年萌生条为试材，研究了不同IBA浓度处理（500、750、1 000、1 250 mg·L-1）对平欧杂交榛根蘖育苗生根的影响，分析了根蘖育苗生根过程中过氧化物酶（POD）、多酚氧化酶（PPO）活性的变化，并对外源激素处理与相关氧化酶活性与生根的关系进行了相关性分析，以期探讨其生根机理。结果表明：IBA浓度处理能显著地提高生根率，以1 000 mg·L-1的生根效果最佳，生根率和不定根质量均显著高于其它处理。生根过程中，POD和PPO活性呈先上升后下降趋势，POD活性在育苗后20 d出现峰值；PPO活性在40 d时达到峰值。生根率与POD、PPO活性呈极显著正相关。说明适宜浓度IBA能增加生根过程POD、PPO活性，从而提高生根率。
- Abstract:
- C.heterophylla×C.avellana (‘Longzhen No.1’) seedlings were used as material.The effects of different IBA concentrations（500，750，1 000，1 250 mg·L-1） on the rooting of C.heterophylla × C.avellana (‘Longzhen No.1’) seedlings were studied,and the dynamic changes of peroxidase(POD)and polyphenol oxidase(PPO)activities during rooting were analyzed.Correlation analysis was conducted in order to discuss the mechanism of rooting.The results showed that the IBA concentration treatment could significantly improve the rooting rate,and 1 000 mg·L-1 had the best rooting effect.The rooting rate and adventitious root quality were significantly higher than those of other treatments.During rooting,the activities of POD and PPO increased first and then decreased,and the activity of POD peaked at 20 days after seedling cultivation,and the activity of PPO peaked at 40 days.Rooting rate was significantly positively correlated with POD and PPO activities.The use of suitable concentration of IBA could increase the activities of POD and PPO in rooting process and increase rooting rate.
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备注/Memo
第一作者简介：逄宏扬（1983-），男，硕士，高级工程师，现主要从事经济林培育等研究工作。E-mail：phy615@163.com.基金项目：黑龙江省森林工业总局应用研究资助项目（sgzjY2014003）。收稿日期：2018-07-30