LUO Zhi,ZHENG Xingjuan,WANG Lihu,et al.Evaluation of Five Natural Variants Materials From Ziziphus jujuba Mill.‘Dongzao’[J].Northern Horticulture,2019,43(01):74-78.[doi:10.11937/bfyy.20181587]
‘冬枣’的五个自然变异材料评价
- Title:
- Evaluation of Five Natural Variants Materials From Ziziphus jujuba Mill.‘Dongzao’
- Keywords:
- Ziziphus jujuba Mill.‘Dongzao’; variant; fruit; phenological period
- 文献标志码:
- A
- 摘要:
- 以‘冬枣’及其5个自然变异材料为试材，对其果实形态、果实营养、物候期、染色体倍性以及DNA 指纹进行了比较分析，以期筛选出综合性状优异的‘冬枣’变异类型。结果表明：与普通‘冬枣’相比，变异1号材料为大果型，其单果质量显著增加，为25.22 g，果实可溶性糖含量和糖酸比提高。在物候期上，变异1号和2号材料与‘冬枣’表现相近，但其它3个变异材料的果实成熟期比‘冬枣’晚约15 d。流式细胞仪分析显示5个自然变异材料均为二倍体，15对SSR引物检测表明5个自然变异材料与‘冬枣’基因组DNA的SSR指纹相同，可能均为‘冬枣’的自然芽变。评价结果显示，变异1号属于优良大果变异，变异3、4、5号为晚熟变异。
- Abstract:
- Ziziphus jujuba Mill.‘Dongzao’ and its five natural variants were taken as materials,comparisons of fruit morphology,fruit nutrition,phonological period,chromosome ploidy and DNA fingerprint were carried out,in order to selecte the variation type of ‘Dongzao’ with excellent comprehensive charaters.The results showed that compared with ‘Dongzao’,the variant No.1 had significantly bigger fruits with the single fruit weight of 25.22 g and higher soluble sugar content and acid-sugar ratio.The phonological period of variation No.1 and No.2 was similar to that of ‘Dongzao’,yet other three variants fruits matured 15 days later than ‘Dongzao’.All the five natural variants were diploidy according to flow cytometry analysis.No difference of DNA fingerprint among ‘Dongzao’ and five natural variants were observed using 15 pairs of SSR primers,which indicated that the five variants were the most probably bud mutations from ‘Dongzao’.The evaluation demonstrated that the variant No.1 was a big fruit type and the variant No.3,No.4 and No.5 belonged to late-maturing fruit type.
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备注/Memo
第一作者简介：罗智（1991-），男，广西贺州人，硕士研究生，研究方向为林木遗传育种。E-mail：Lz15930762701@126.com.责任作者：王玖瑞（1972-），男，河北丰南人，博士，教授，研究方向为林木遗传育种。E-mail：wjrjujube@126.com.基金项目：河北省科技计划资助项目（16226313D-1）；北京市2011协同创新中心资助项目（PXM2018_014207_000024）;天津市科技计划资助项目（17ZXBFNC00210）。收稿日期：2018-07-03