[1]马丽,王庆军,罗华,等.枣庄石榴种质资源遗传多样性的ISSR分析[J].北方园艺,2018,42(24):35-40.[doi:10.11937/bfyy.20181599]
　MA Li,WANG Qingjun,LUO Hua,et al.Genetic Diversity Analysis of Pomegranate Germplasm Resources From Zaozhuang by ISSR Markers[J].Northern Horticulture,2018,42(24):35-40.[doi:10.11937/bfyy.20181599]
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枣庄石榴种质资源遗传多样性的ISSR分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年24 页码: 35-40 栏目: 出版日期: 2018-12-25
Title:
Genetic Diversity Analysis of Pomegranate Germplasm Resources From Zaozhuang by ISSR Markers
作者:
马丽1; 王庆军2; 罗华2; 郝兆祥2; 周玉亮1; 赵丽娜2
（1.枣庄学院 生命科学学院,山东 枣庄 277160；2.枣庄市石榴研究中心,枣庄市峄城区果树中心,山东 枣庄 277300）
Author(s):
MA Li1; WANG Qingjun2; LUO Hua2; HAO Zhaoxiang2; ZHOU Yuliang1; ZHAO Lina2
(1.Life Science Department,Zaozhuang College,Zaozhuang,Shandong 277160;2.Zaozhuang Pomegranate Research Center/Yicheng District Fruit Tree Center in Zaozhuang,Zaozhuang,Shandong 277300)
关键词:
枣庄; 石榴; 遗传多样性; 遗传关系; ISSR
Keywords:
Zaozhuang; pomegranate（Punica granatum L.）; genetic diversity; genetic relationships; ISSR
DOI:
10.11937/bfyy.20181599
文献标志码:
A
摘要:
以39份枣庄石榴品种为试材，采用ISSR标记方法，研究了石榴品种间的遗传多样性及遗传关系，以期为枣庄石榴种质资源保存、杂交育种奠定基础。结果表明:9条ISSR引物对39个品种进行扩增，共检测到168个DNA位点，平均每条引物扩增出18.67个DNA位点，其中多态性位点156个，多态性位点比率（PPB）为92.86%。供试样品平均观察等位基因数（Na）、有效等位基因数（Ne）、Nei′s 基因多样性指数（He）及Shannon信息指数（I）分别为1.928 6、1.690 9、0.384 7、0.557 3；品种的遗传相似系数（GS）在0.410 7～0.958 3，采用UPGMA 构建遗传关系聚类图，以遗传相似性系数（GS）0.656为阈值，供试的39份石榴品种可分为5类，根据ISSR标记的品种分类结果与根据形态性状分类结果不一致。
Abstract:
The 39 pomegranate cultivars from Zaozhuang were used as experimental materials in this study.The genetic relationships and genetic diversity of 39 pomegranate cultivars were analysed by ISSR markers in order to provided the foundation on the storage and breeding of the pomegranate resources at Zaozhuang.The results showed that a total of 168 loc were amplified from 9 ISSR primers with an average of 18.67 bands from a primer,of which 156 were polymorphic loci.The main indexes were,that percentage of polymorphic band (PPB)＝92.86%，the number of alleles (Na)=1.928 6，Effective number of alleles (Ne)=1.690 9,Nei′s gene diversity (He)=0.384 7 and Shannon′s information index (I)=0.557 3.The genetic similarities coefficient (GS) between cultivars ranged from 0.410 7 to 0.958 3,39 pomegranate cultivars were divided into 6 groups at GS 0.656 based on UPGMA method.The results of ISSR cluster did not accord with the classification results based on some morphological variations.

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备注/Memo

第一作者简介：马丽（1980-），女，博士，副教授，研究方向为石榴种质资源分子评价。E-mail:mary1976816@163.com.基金项目：山东省高等学校科技计划资助项目（J14LF02）；国家级大学生创新创业训练计划资助项目（201610904058）；山东省农业科技发展资金资助项目（2015）；枣庄英才集聚工程资助项目（枣政办字[2016]23号）。收稿日期：2018-07-03

更新日期/Last Update: 2019-01-22