[1]梁姗,杨霞.基于ITS序列的胭脂萝卜亲缘关系分析[J].北方园艺,2019,43(01):30-35.[doi:10.11937/bfyy.20181377]
　LIANG Shan,YANG Xia.Analysis of Carmine Radish Genetic Relationship Based on ITS Sequences[J].Northern Horticulture,2019,43(01):30-35.[doi:10.11937/bfyy.20181377]
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基于ITS序列的胭脂萝卜亲缘关系分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年01 页码: 30-35 栏目: 出版日期: 2019-01-15
Title:
Analysis of Carmine Radish Genetic Relationship Based on ITS Sequences
作者:
梁姗; 杨霞
（长江师范学院 现代农业与生物工程学院，重庆 408100）
Author(s):
LIANG Shan; YANG Xia
(College of Modern Agriculture and Biological Engineering,Yangtze Normal University，Chongqing 408100)
关键词:
胭脂萝卜; ITS序列; 遗传距离; 亲缘关系
Keywords:
carmine radish; ITS sequence; genetic distance; genetic relationship
DOI:
10.11937/bfyy.20181377
文献标志码:
A
摘要:
以胭脂萝卜为试材，采用PCR扩增其ITS1-5.8S rRNA-ITS2序列，研究了序列同源性并构建系统发育树，以期为胭脂萝卜亲缘关系分析提供参考依据。结果表明：胭脂萝卜ITS1-5.8S rRNA-ITS2序列总长度为655 bp，ITS1（G+C）含量为51.4%，长度为220 bp；5.8S rRNA（G+C）含量为53.4%，长度为217 bp；ITS2（G+C）含量为55.1%，长度为218 bp。胭脂萝卜与台北的Mei-Hwa、南京的Cd-03、台北的Mei-Nong、南京的Yanghua、杭州的Baiyuchun、渥太华的SW77的同源性最高（99%），归为同一种。由系统发育树可得20个样品的总平均遗传距离是0.134，结合形态学特征表明待测胭脂萝卜可能由白皮白心萝卜进化而来。
Abstract:
To detect the genetic relationship of carmine radish,carmine radish was taken as the material,the complete ITS1-5.8S rRNA-ITS2 sequence was amplified by PCR,and homology analysis and phylogenetic tree were constructed.The results showed the length of ITS1-5.8S rRNA-ITS2 sequences was 655 bp,the （G+C）content of ITS1 （220 bp）was 51.4%,5.8S rRNA（217 bp） was 53.4%，ITS2 （218 bp）was 55.1%.The homology analysis showed carmine radish,Taipei（Mei-Hwa）,Nanjing（Cd-03）,Taipei（Mei-Nong）,Nanjing（Yanghua）,Hangzhou（Baiyuchun）,and Ottawa（SW77） belonged to the same species with the highest homology （99%）.The phylogenetic tree showed the average genetic distance of the 20 samples was 0.134.Combined with morphological features indicated that carmine radish may be evolved from radish with white episperm and white heart.

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备注/Memo

第一作者简介：梁姗(1982-)，女，博士，讲师,现主要从事天然产物活性等研究工作。E-mail:vmm420@Hotmail.com.基金项目：重庆市生物工程与现代农业专业群资助项目（CSZKY1809）；长江师范学院科研基金资助项目（2015KYQD01）。收稿日期：2018-08-01

更新日期/Last Update: 2019-01-23