[1]何彩梅,吴桂容,蒋英军,等.柑橘果酒富硒酵母的选育及其富硒条件优化[J].北方园艺,2018,42(14):125-132.[doi:10.11937/bfyy.20180431]
HE Caimei,WU Guirong,JIANG Yingjun,et al.Selective Breeding and Optimization of Selenium-enriched Yeast for Production of Wine From Citrus Fruit[J].Northern Horticulture,2018,42(14):125-132.[doi:10.11937/bfyy.20180431]
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HE Caimei,WU Guirong,JIANG Yingjun,et al.Selective Breeding and Optimization of Selenium-enriched Yeast for Production of Wine From Citrus Fruit[J].Northern Horticulture,2018,42(14):125-132.[doi:10.11937/bfyy.20180431]
柑橘果酒富硒酵母的选育及其富硒条件优化
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
卷:
第42卷
期数:
2018年14
页码:
125-132
栏目:
出版日期:
2018-07-25
- Title:
- Selective Breeding and Optimization of Selenium-enriched Yeast for Production of Wine From Citrus Fruit
- Keywords:
- citrus fruit wine; selenium-enriched yeast; selective breeding; optimization of fermentation conditions
- 文献标志码:
- A
- 摘要:
- 以柑橘果汁为自然发酵原料，结合有机硒含量及感官评价结果，采用亚硒酸钠抗性平板筛选与紫外诱变的方法，选育得到一株富硒能力较强的酵母菌B1，通过单因素分析的方法对该富硒酵母的发酵条件进行优化。结果表明：酵母菌B1富硒发酵最适亚硒酸钠浓度为75 μg·mL-1，在此最佳硒浓度下，当菌株接种量为5%，发酵液初始pH为4.4，在装液量为80%的摇瓶（250 mL）中32 ℃发酵3 d后，其总硒含量可达4 756.76 μg·L-1，同时该菌株的生物量可高达2.57 g·L-1。
- Abstract:
- A high selenium-enriched yeast B1 was isolated from the naturally-fermented orange juice by ultraviolet mutagenesis and screening on sodium selenite resistant plate,combining with the selenium concentration and sensory test.And the fermentation conditions of selenium-enriched yeast B1 were optimized by single factor analysis.The results showed that the yeast B1 showed optimal selenium-enriched concentration at 75 μg·mL-1,under this optimal selenium concentration,the optimal fermented conditions for high selenium enrichment was 3 days at 32 ℃ with pH 4.4,when inoculation amount was 5% in a shaker（250 mL）containing 80% citrus juice.The total selenium content was up to 4 756.76 μg·L-1,and the biomass of yeast B1 could be as high as 2.57 g·L-1 after the optimization.
备注/Memo
第一作者简介：何彩梅（1984-），女，硕士，讲师，研究方向为农产品加工与检测。E-mail：shwjs@126.com.责任作者：龚福明（1982-），男，硕士，副教授，研究方向为食品微生物与食品安全。E-mail：gongfuming017@hotmail.com.基金项目：贺州市科学研究与技术开发计划资助项目（贺科攻1707023,贺科攻1707024）；广西壮族自治区中青年教师基础能力提升资助项目（KY2016LX377）；大学生创新创业资助项目（201611838034）；云南省教育厅科学研究基金资助项目（2017ZZX109）；广西果蔬保鲜和深加工研究人才小高地资助项目。收稿日期：2018-03-12
更新日期/Last Update:
2018-08-07