MO Xiaofeng,WEN Qinglan,QIN Liping,et al.Improving Heat-resistance of Rhododendron hybridum by Using Salicylic Acid Combined With Abscisic Acid During High Temperature Stress[J].Northern Horticulture,2018,42(12):1-6.[doi:10.11937/bfyy.20174127]
外源SA和ABA复合处理提高杜鹃花叶片耐热性
- Title:
- Improving Heat-resistance of Rhododendron hybridum by Using Salicylic Acid Combined With Abscisic Acid During High Temperature Stress
- Keywords:
- Rhododendron hybridum; heat stress; salicylic acid; abscisic acid; antioxidant enzyme activity
- 文献标志码:
- A
- 摘要:
- 以生长一致2年生的胭脂蜜盆栽杜鹃花为试材，以喷施蒸馏水为对照(CK)，采用SA、ABA及SA+ABA复合处理喷施2次叶片后，研究了SA和ABA处理对39/29 ℃高温胁迫对叶片抗氧化和耐热性的影响，以期通过喷施SA和ABA来提高杜鹃花耐热能力。结果表明：与对照相比，经SA、ABA和SA+ABA复合处理均可提高叶片可溶性总糖、脯氨酸含量、相对含水量和叶绿素含量，降低叶片的相对电导率；促进叶片抗氧化酶SOD和CAT活性增加，降低叶片H2O2和细胞膜脂过氧化产物MDA含量。但以SA+ABA复合处理对提高杜鹃花的耐热性效果最好，ABA次之，然后是SA。因此，SA+ABA复合处理更能促进渗透物质合成，提高抗氧化能力和细胞完整性，从而提高对高温胁迫抗性。
- Abstract:
- To explore the effects of exogenous Salicylic acid (SA) and Abscisic acid (ABA) treatment on heat resistance of Rhododendron hybridum. 2-year old Rhododendron hybridum ‘Yanzhimi’ were sprayed with 0 (Control), 10.0 μmol·L-1 SA, 30.0 μmol·L-1 ABA and 10.0 μmol·L-1 SA+30.0 μmol·L-1 ABA solution on the leaves and roots for two days, respectively. And then treated with heat stress (39 ℃/29 ℃,day/night) for 8 days by using plant growth chamber. The total chlorophyll content, osmotic regulation substances, membrane lipid peroxidation, and antioxidant enzymes activity of leaves were measured after different treatment times. The results showed that, compare to the control, treated with SA, ABA and SA+ABA could markedly improved the leaves of total soluble sugar, proline, relative water and chlorophyll content, and decreased relative electrical conductivity, Malondialdehyde (MDA) and H2O2 content in leaves. Meanwhile, improved activities of SOD and CAT in leaves. However, compared with the control, treated SA coupled with ABA had the best effect improving heat resistance for Rhododendron hybridum, and then was treated with ABA and SA. These results showed that treat with SA+ABA can improve osmotic regulation substances content, inhibiting water loss, chlorophyll degradation and membrane oxidation, enhance the resistance to high temperature stress and alleviate the negative injuries. Keywords：Rhododendron hybridum；heat stress；salicylic acid；abscisic acid；antioxidant enzyme activity
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MO Xiaofeng,WEN Qinglan,QIN Liping,et al.Improving Heat-resistance of Rhododendron hybridum by Using Salicylic Acid Combined With Abscisic Acid During High Temperature Stress[J].Northern Horticulture,2018,42(12):87.[doi:10.11937/bfyy.20174127]
备注/Memo
第一作者简介：莫小锋（1979-），男，本科，林业工程师，现主要从事园林树木抗性生理研究工作。E-mail：156655266@qq.com. 责任作者：莫亿伟（1971-），男，博士，副教授，现主要从事植物生理研究工作。E-mail：455615189@qq.com. 基金项目：浙江省自然科学基金资助项目（LY14C130003）。收稿日期：2018―02―01