[1]陈林,廖彦,黄威,等.茯苓胞外多糖、胞内多糖与菌核多糖的抗氧化活性比较研究[J].北方园艺,2018,42(02):172-177.[doi:10.11937/bfyy.20171329]
　CHEN Lin,LIAO Yan,HUANG Wei,et al.Comparative Study on Antioxidant Activity of Extracellular Polysaccharide,Intracellular Polysaccharide and Sclerotial Polysaccharide of Poria cocos[J].Northern Horticulture,2018,42(02):172-177.[doi:10.11937/bfyy.20171329]
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茯苓胞外多糖、胞内多糖与菌核多糖的抗氧化活性比较研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年02 页码: 172-177 栏目: 出版日期: 2018-01-25
Title:
Comparative Study on Antioxidant Activity of Extracellular Polysaccharide,Intracellular Polysaccharide and Sclerotial Polysaccharide of Poria cocos
作者:
陈林; 廖彦; 黄威; 张书航; 鲁耀邦; 崔培梧
湖南中医药大学 药学院,湖南 长沙 410208
Author(s):
CHEN Lin; LIAO Yan; HUANG Wei; ZHANG Shuhang; LU Yaobang; CUI Peiwu
College of Pharmacy,Hunan University of Chinese Medicine,Changsha,Hunan 410208
关键词:
茯苓多糖; 抗氧化活性; 红外光谱分析
Keywords:
polysaccharide of Poria cocos; antioxidant activity; infrared spectroscopy
DOI:
10.11937/bfyy.20171329
文献标志码:
A
摘要:
为探索不同来源茯苓多糖的抗氧化活性，采用邻二氮菲-Fe2+氧化法、DPPH自由基分析法、邻苯三酚自氧化法分别考察了茯苓胞外多糖、胞内多糖和菌核多糖对羟基自由基（?OH）、DPPH自由基（DPPH?）和超氧阴离子自由基（O?[TX-*4]2）的清除活性，采用傅里叶变换红外光谱分析法对其结构进行了初步分析。结果表明：不同来源的茯苓多糖均表现出3种自由基的清除活性，但茯苓胞内多糖的活性最佳，其对?OH、DPPH?和O?[TX-*4]2的最大清除率分别为32 07%、90 12%和39 74%。红外光谱分析进一步验证了3种待测样品均为多糖，但茯苓胞内多糖在823 cm-1处存在特征吸收峰，推测其结构中存在独特的甘露糖苷键。这些数据证实了茯苓多糖的抗氧化活性以及茯苓胞内多糖在抗氧化保健食品和药品研发领域的应用优势，为真菌多糖的深度开发提供了必要参考。
Abstract:
In order to explore the antioxidant activity of different pachymaran,phenanthroline Fe2+ method,DPPH? assay method and pyrogallic acid self-oxidizing method were employed for analyzing ?OH scavenging activity,DPPH? scavenging activity and super oxygen anion free radical scavenging activity of extracellular polysaccharide,intracellular polysaccharide and sclerotial polysaccharide of Poria cocos.Then Fourier transform infrared spectrometry was also used to identify the structural difference among the three selected pachymaran.The results indicated that all the three pachymaran could exhibit scavenging activity of ?OH,DPPH? and O?[TX-*4]2,but intracellular polysaccharide showed the best antioxidant activity with ?OH scavenging activity of 32 07%,DPPH?scavenging activity of 90 12%,and super oxygen anion free radical scavenging activity of 39 74%,respectively.Analysis of infra-red spectra suggested that all the tested samples belonged to polysaccharide,and a special absorption peak at 823 cm-1 was only found in intracellular polysaccharide,which might imply the existence of mannose glycosidic bond.These data verified antioxidant activity of pachymaran and advantage of intracellular polysaccharide of Poria cocos in research and development of food and drug with antioxidant activity,and also provided a necessary reference for deep exploitation of fungal polysaccharide.

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备注/Memo

第一作者简介：陈林（1989-），女，山东菏泽人，硕士研究生，研究方向为药用菌物综合开发与利用。E-mail：976931186@qq.com.

责任作者：崔培梧（1983-），男，博士，讲师，研究方向为药用菌物综合开发与利用。E-mail：cuipeiwu@126.com.
基金项目：湖南省科技厅创新平台与人才计划资助项目（2015JC3073）；湖南省中医药科研计划重点资助项目（201621）；长沙市科技计划资助项目（kq1701069）；湖南省芦笋产业联合开发基金资助项目（060100990111）；湖南中医药大学研究性学习和创新性实验计划资助项目（201616）；湖湘中药资源保护与利用协同创新中心开放基金资助项目（00175-12）；湖南中医药大学生物工程重点学科资助项目（校行科[2012]2号）。
收稿日期：2017-07-18

更新日期/Last Update: 2018-01-26