CHEN Rui,SUN Xiaoyu,LU Pengpeng,et al.Effect of Planting Years and Growth Stages on Soil Microbial Metabolic Functions in Greenhouse[J].Northern Horticulture,2017,41(11):179-185.[doi:10.11937/bfyy.201711038]
不同棚龄及种植期对土壤微生物代谢功能的影响
- Title:
- Effect of Planting Years and Growth Stages on Soil Microbial Metabolic Functions in Greenhouse
- 关键词:
- 细菌; Biolog ECO平板; 微生物群落; 代谢功能
- Keywords:
- bacteria; Biolog ECO plate; microbial communities; metabolic function
- 文献标志码:
- A
- 摘要:
- 为了解不同年限（3、6、9、15年）及不同种植期（定植期、坐果期、盛果期、拉秧期）的设施棚室土壤微生物对碳源利用的变化趋势，对土壤理化性质和土壤微生物碳源利用进行了检测分析。结果表明：种植年限增加导致棚室土壤pH约降低0.52，土壤可溶性盐分、总磷及总碳随着棚龄增加逐渐累积。不同棚龄土壤中，9年棚室微生物碳源代谢活性最高，15年棚室有所下降，但差别不显著。与不同棚龄棚室土壤代谢差异显著相关的碳源有15种。不同种植期土壤样本中，微生物群落对各类碳源代谢均呈现坐果期最强，拉秧期最弱的特征，并且差异显著，与其代谢差异显著相关的碳源19种。
- Abstract:
- In order to indicate the change of microbial metabolic function in different planting years(3，6，9 and 15 years) and different growth stages(planting，bear fruit，productive and uproot) in greenhouse，physical-chemical properties and microbes carbon source utilization were investigated.The results showed that soil pH dropped about 0.52 units.The water soluble salt，total phosphorus and total carbon were accumulated in greenhouse soil with the increase of shelf age.The microbial metabolic of the carbon source was the highest in the 9 years soil，and changed little at 15 years，but there was no significant difference.There were 15 types of carbon sources had significant correlation with microbial metabolic activity between the different planting years.Microbial metabolic of the carbon source at uproot period were the lowest and significant difference with planting，bear fruit and productive period，there were 19 types of carbon sources had significant correlation with microbial metabolic activity between the plant growth stage in greenhouse.
参考文献/References:
[1]钟修洪,叶剑波.设施连作障碍产生的原因及其防治措施[J].现代园艺,2011(1):48-49. [2]黄春生,熊明.连作障碍的产生原因及改善途径[J].上海蔬菜,2010(5):62-64. [3]李文娇,杨殿林,赵建宁,等.长期连作和轮作对农田土壤生物学特性的影响研究进展[J].中国农学通报,2015(3):173-178. [4]张杰,余潮,王自海,等.不同植被群落表层土壤中细菌群落多样性[J].环境科学研究,2013,6(8):866-872. [5]李晓慧,韩晓增,王树起,等.土壤微生物对不同作物根系活动的响应[J].土壤与作物,2010,26(2):156-159. [6]E KANDELER,D TSCHERKO,H SPIEGEL.Long-term monitoring of microbial biomass,N mineralisation and enzyme activities of a Chernozem under different tillage management[J].Biology & Fertility of Soils,1999,28(4):343-351. [7]李贺勤,李星月,刘奇志,等.连作障碍调控技术研究进展[J].北方园艺,2013(23):193-197. [8]吴洪生,周晓冬,闫霜,等.拮抗菌与有机肥配合防治黄瓜连作障碍及提高黄瓜品质研究[J].西南农业学报,2013,4(26):1604-1607. [9]檀国印,杨志玲,袁志林,等.植物根际促生菌及其在克服连作障碍中的潜力[J].热带作物学报,2013(1):135-141. [10]JAY L G.Analysis and interpretation of community-level physiological profiles in microbial ecology[J].Microbiology Ecology,1997,24(4):289-300. [11]土壤干物质和水分的测定-重量法：HJ 613-2011[S].北京:中国环境科学出版社,2011:1-5. [12]土壤pH的测定：NY-T 1121.2[S].北京:中国农业出版社,2015:1-5. [13]土壤检测-第16部分:土壤水溶性盐总量的测定：NY-T 1121.16[S].北京:中国农业出版社,2015:1-5. [14]土壤全磷测定法：NY/T88-1988[S].北京:中华人民共和国农业部,1989:1-3. [15]土壤全钾测定法：NY/T 87-1988[S].北京:中华人民共和国农业部,1989:1-5. [16]土壤有机质测定法：NY/T 85-1988[S].北京:中华人民共和国农业部,1989:1-6. [17]Soil quality-Sampling-Part 6:Guidance on the collection,andling and storage of soil under aerobic conditions for the assessment of microbiological processes,iomass and diversity in the laboratory：ISO 10381-6-2009[S].英国:英国标准协会,2010:1-6. [18]王强,戴九兰,吴大千,等.微生物生态研究中基于BIOLOG方法的数据分析[J].生态学报,2010,30(3):817-823. [19]张文霖.主成分分析在SPSS中的操作应用[J].市场研究,2005(12):31-34. [20]郭晓冬.设施栽培条件下土壤的连作障碍及防治措施[J].甘肃农业科技,2003(7):38-40. [21]帅正彬,李杰.蔬菜连作障碍与综防措施研究进展[J].中国园艺文摘,2014(10):60-63. [22]YANG Y H,YAO J,HUA X M.Effect of pesticide pollution against functional microbial diversity in soil[J].J Microbiol,2000,20(2):44-47.
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备注/Memo
第一作者简介：陈锐（1977-），女，博士，副研究员，研究方向为环境微生物多样性及土壤生物修复。E-mail：chenrui@sxim.ac.cn.基金项目：陕西省科学院青年人才培养资助项目（2013K-25）；陕西省农业科技创新与攻关资助项目（2015NY088）。