ZHU Weihong,MIAO Xiaoyan,ZHANG Xiaomei,et al.Breeding Research of Ultrasonic-high Temperature Inducement on Agrocybe aegerita Huang Chalmydospore[J].Northern Horticulture,2017,41(04):132-136.[doi:10.11937/bfyy.201704031]
超声-高温诱导茶薪菇厚垣孢子育种研究
- Title:
- Breeding Research of Ultrasonic-high Temperature Inducement on Agrocybe aegerita Huang Chalmydospore
- Keywords:
- Agrocybe aegerita Huang; degenerate strains; chlamydospore; induced
- 文献标志码:
- A
- 摘要:
- 以茶薪菇SM(三明原株)、At103（退化株）2种菌株分离出的厚垣孢子为新型育种材料，采用超声波-高温复合处理,比较各处理间菌丝长势及出菇情况，探讨茶薪菇厚垣孢子诱导育种的可行性。结果表明：超声-高温处理对厚垣孢子育种具有明显促进作用，以超声波300 W、15 min，复合40 ℃高温处理6 h效果较好，其中S3菌株增产29.6%。退化不出菇菌株厚垣孢子诱导后可分化形成子实体，生物转化率30%。
- Abstract:
- Chlamydospores of Agrocybe aegerita Huang isolated from two strains SM and At103 were used as new materials to study the feasibility of inducing breeding.After ultrasonic-high temperature treatments of chlamydospores.Mycelium growing and fruiting among different processing were compared.The results showed that ultrasonic-high temperature treatments had obvious promotion effect on inducing breeding,the best effect was under the conditions of 300 W ultrasonic+15 minutes，40 ℃+6 hours,the induced dominant strains S3 increased yield by 29.6%，and with the inducing treatments of chlamydospores，the undifferentiated strain could form fruiting bodies again,the biological conversion rate could reach 30%.
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备注/Memo
第一作者简介：朱维红（1970-），女，本科，高级实验师，研究方向为微生物应用。E-mail：zhuweihonglxn@126.com.责任作者：张筱梅（1957-），女，本科，教授，研究方向为食用真菌。E-mail:zhxm06@163.com.基金项目：河北省自然科学基金资助项目(C2013104055)；保定学院食药用菌驯化育种与开发科研团队资助项目（KXT02013007）。