[1]南海风,刘欢,白杨,等.能量调控下草莓果实衰老过程中活性氧与超微弱发光的关系[J].北方园艺,2017,41(20):67-71.[doi:10.11937/bfyy.20164916]
　NAN Haifeng,LIU Huan,BAI Yang,et al.Relationship Between Ultraweak Photon Emissio and Reactive Oxygen Under Regulating Energy in Ageing Process of Strawberry Fruit[J].Northern Horticulture,2017,41(20):67-71.[doi:10.11937/bfyy.20164916]
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能量调控下草莓果实衰老过程中活性氧与超微弱发光的关系

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年20 页码: 67-71 栏目: 出版日期: 2017-10-30
Title:
Relationship Between Ultraweak Photon Emissio and Reactive Oxygen Under Regulating Energy in Ageing Process of Strawberry Fruit
作者:
南海风1; 刘欢2; 白杨2; 闫宇彤2; 任鹏达2; 郭金丽2
1.内蒙古建筑职业技术学院 建筑与规划学院，内蒙古 呼和浩特 010070；2.内蒙古农业大学 农学院，内蒙古 呼和浩特 010018
Author(s):
NAN Haifeng1; LIU Huan2; BAI Yang2; YAN Yutong2; REN Pengda2; GUO Jinli2
1.Department of Architecture and Planning,Inner Mongolia Technical College of Construction,Hohhot,Inner Mongolia 010070;2.College of Agronomy,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018
关键词:
草莓果实; 衰老; 能量调控; 超微弱发光; 活性氧
Keywords:
strawberry fruit; ageing; regulating energy; ultraweak photon emissio; reactive oxygen
DOI:
10.11937/bfyy.20164916
文献标志码:
A
摘要:
以‘红颜’草莓果实为试材，采用呼吸链解偶联剂2，4-二硝基苯酚(DNP）和三磷酸腺苷(ATP）处理草莓果实，以蒸馏水浸泡为对照，对比分析抑制能量生成和增加能量条件下草莓果实衰老过程中超微弱发光(ultraweak photon emissio,UPE)和活性氧的变化及二者之间的关系。结果表明：在草莓果实采后衰老过程中，DNP处理、ATP处理和对照的超氧阴离子(O2?-）产生速率、丙二醛(MDA）含量和相对电导率均持续上升；同时三者的UPE强度均整体下降。在整个果实衰老过程中，DNP处理的O2?-产生速率、MDA含量和相对电导率均高于对照，ATP处理后三者均低于对照。相关性分析也表明，UPE强度与活性氧水平呈显著负相关。由以上结果可见，抑制ATP生成情况下加剧了草莓果实活性氧上升积累，同时加剧了UPE强度下降；增加ATP则减缓了活性氧上升积累，同时减缓了UPE强度下降。UPE强度随着活性氧的增加积累而下降。以上结果表示活性氧并不是引发UPE的直接来源，而是间接影响了UPE。
Abstract:
The study aimed to understand the change of ultraweak photon emission(UPE) and reactive oxygen,and the relationship between UPE and reactive oxygen by soaking fruit with 2,4-dinitrophenol (DNP) and adenosine triphosphate(ATP) in ageing process of ‘Hongyan’ strawberry fruit.The results showed that,during fruit ageing process,superoxide anion radical(O2?-）production rates,malondialdehyde (MDA) content and relative electric conductivity increased continuously under three treatments of ATP,DNP and CK.Meanwhile UPE intensity of three treatments decreased.Furthermore,in whole ageing process,O2?- production rate,MDA content and relative electric conductivity of DNP treatment were all higher than that of CK but UPE intensity was lower than that of CK.While O?[TX-*4]2 production rate,MDA content and relative electric conductivity of ATP treatment were all lower than that of CK but UPE intensity was higher than that of CK.Correlation analysis showed that UPE intensity had a significantly negative correlation with reactive oxygen level.Above results stated that inhibiting ATP intensified the accumulation of reactive oxygen and meanwhile intensified the decline of UPE intensity.However,increasing ATP relieved the accumulation of reactive oxygen and the decline of UPE intensity.UPE intensity declined along with the accumulation of reactive oxygen.The results indicated that reactive oxygen was not the direct source for UPE but could effect UPE indirectly.

参考文献/References:

[1]GURWITSCH A.Die natur des spezifischen erregers der zellteilung[J].Archiv für mikroskopische Anatomie und Entwicklungsmechanik,1923,100(1):11-40.

[2]POPP F A,LI K H,GU Q.Recent advances in biophoton research and its applications[M].World Scientific Pub Co Inc,1992:1-46.
[3]袁佐清.生物超微弱发光研究进展[J].安徽农业科学,2008,36(8):3092-3094.
[4]胡献明,潘建伟,陈虹,等.铝胁迫诱导大麦根尖细胞超微弱发光的变化[J].浙江大学学报,2002,28(4):383-386.
[5]侯仙慧,廖祥儒,李颖,等.苋菜种子萌发过程的超微弱发光及其机理研究[J].种子,2004,23(7):23-27.
[6]刘红梅,廖祥儒,吴立峰,等.热休克对小麦未成熟种子萌发、生物发光和抗氧化酶活性的影响[J].食品与生物技术学报,2006,25(3):75-78.
[7]林桂玉,黄在范,张翠华,等.菊花花芽分化期超微弱发光及生理代谢的变化[J].园艺学报,2008,35(12):1819-1824.
[8]YU Y,WANG J.Ultraweak bioluminescence and vigour of irradiated rice[J].Int J Agric & Biol Eng,2010,3(1):85-90.
[9]赵丹莹,生吉萍,丁洋,等.超微弱发光用于番茄果实冷害发生程度的无损监测[J].光谱学与光谱分析,2010,30(9):2493-2495.
[10]张菊平,张兴志,巩振辉.超微弱发光在蔬菜研究中的应用[J].中国农学通报,2006,22(1):220-222.
[11]樊秀彩,关军锋,张继澍,等.草莓采后微粒体膜Ca2+-ATPase活性与膜脂过氧化水平[J].园艺学报,2003,30(1):15-18.
[12]陈学红,郑永华,杨震峰,等.高氧处理对草莓果实采后活性氧代谢和腐烂的影响[J].南京农业大学学报,2005,28(1):99-102.
[13]GUAN J,GAO M,FAN X,et al.The relationship between maturation and senescence of strawberry fruit and Ca2+,CaM,Ca2+-ATPase and activate oxygen metabolism[J].Scientia Agricultura Sinica,2002,35(11):1385-1389.
[14]陈文烜,宋丽丽,廖小军.减压贮藏技术对水蜜桃采后能量代谢的影响[J].农业机械学报,2014,45(10):226-230.
[15]李玲.植物生理学模块实验指导[M].北京:科学出版社,2009.
[16]孙群,胡景江.植物生理学研究技术[M].杨凌:西北农林科技大学出版社,2006.
[17]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2004.
[18]张新华,杨洪强.植物叶绿体和线粒体的超微弱发光[J].植物生理学通讯,2004,40(1):111-114.
[19]张新华,杨洪强,李富,等.杏花开放过程中超弱发光和ATP及活性氧含量的变化[J].植物生理与分子生物学学报,2004,30(1):41-44.
[20]刘欢,朱冠宇,梁爽,等.草莓果实采后衰老过程中果实品质及超微弱发光的变化[J].北方农业学报,2016,44(3):29-32.
[21]郭金丽,朱冠宇,刘艳,等.草莓果实衰老过程中超微弱发光的变化及与活性氧的关系[J].北方园艺,2016(11):1-4.

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备注/Memo

第一作者简介：南海风(1983-），女，硕士，讲师，现主要从事园林园艺植物栽培与应用等研究工作。E-mail：2106-2884@qq.com.

责任作者：郭金丽(1972-），女，博士，副教授，现主要从事园艺植物生理和栽培及植物超微弱发光等研究工作。E-mail：guojinli1111@163.com.
基金项目：国家自然科学基金资助项目(31260455)。
收稿日期：2017-06-22

更新日期/Last Update: 2017-11-01