GE Honglian,JI Xiue.Screening，Identification and Promoting Effects of Potassium-solubilizing Bacteria in Rhizosphere of Cucumber[J].Northern Horticulture,2017,41(13):21-25.[doi:10.11937/bfyy.20164683]
黄瓜根际解钾细菌的分离筛选、鉴定及其促生效果
- Title:
- Screening，Identification and Promoting Effects of Potassium-solubilizing Bacteria in Rhizosphere of Cucumber
- Keywords:
- cucumber; K-solubilizing baceria; rhizosphere; screening; growth promotion
- 文献标志码:
- A
- 摘要:
- 以黄瓜根际土壤为试材，采用亚历山大硅酸盐细菌培养基从黄瓜根际土壤中分离筛选解钾菌，用原子吸收法测定菌株的解钾能力，选出解钾能力较强的菌株进行菌种鉴定，同时研究其对黄瓜幼苗生长的影响。结果表明：从黄瓜根际土壤中共分离获得9株细菌，其中PK1、PK3和PK7具有较强的解钾能力，液体培养96 h后其解钾率分别为30.05%、33.78%和44.01%。依据菌株的形态学特征和生理生化特征鉴定，PK1和PK3为恶臭假单胞菌（Pseudomones putida），PK7为胶质类芽孢杆菌（Paenibacillus mucilaginosus）。盆栽试验结果表明，与对照相比，14 d后PK1提高黄瓜的根长、株高、鲜质量和干质量分别为42.60%、10.45%、48.65%、33.01%，PK3分别对应提高65.26%、21.09%、83.78%和49.48%，PK7分别对应提高79.46%、44.26%、105.41%和113.03%。
- Abstract:
- Rhizosphere soil of cucumber was used as test material，K solubilizing bacteria were isolated in Alexandrove medium.The ability of dissolving potassium of K solubilizing bacteria were determined with the atomic absorption spectrophotometer，then the strains with stronger K solubilizing bacteria were identified，meanwhile，the effects on the growth of cucumber seedling were studied.The results showed that nine K solubilizing bacterium strains were isolated from cucumber rhizosphere soil,in which PK1，PK3 and PK7 demonstrated strong potassium decomposing ability，and potassium decomposing rate of them cultivated in liquid medium for 96 hour was 30.05%,33.78% and 44.01%，respectively.Based on the morphological and physiological and biochemical characterestics，PK1 and PK3 were identified into Pseudomones putida，PK7 was Paenibacillus mucilaginosus.In pot experiment，after 14 days PK1 increased the root length，plant height as well as fresh weight and dry weight of cucumber seedling by 42.60%，10.45%，48.65% and 33.01%，PK3 increased the correspondents by 65.26%，21.09%，83.78% and 49.48%，and PK7 increased the correspondents by 79.46%，44.26%，105.41% and 113.03%，respectively，compared with the control.
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备注/Memo
第一作者简介：葛红莲（1976-），女，硕士，副教授，研究方向为微生物农药和微生物菌肥的开发与应用。E-mail:gehonglian2003@126.com.基金项目：河南省科技厅科技攻关资助项目(162102310588)；周口师范学院校本资助项目(ZKNUB215210)。