ZHAO Jinfeng,TIAN Chunyu,GUO Dongxue,et al.Cloning and Expression Analysis for Copper Tolerance Related Gene CsLecRK in Cucumber[J].Northern Horticulture,2017,41(13):14-21.[doi:10.11937/bfyy.20164582]
黄瓜耐铜相关基因CsLecRK的克隆及表达分析
- Title:
- Cloning and Expression Analysis for Copper Tolerance Related Gene CsLecRK in Cucumber
- Keywords:
- cucumber; copper tolerance related gene; CsLecRK; expression analysis
- 文献标志码:
- A
- 摘要:
- 以黄瓜为试材，根据基因响应铜（Cu）的表达模式及保守性，采用基因克隆和生物信息学方法，对筛选获得的黄瓜凝集素受体激酶基因（CsLecRK）进行克隆及生物信息学分析，并对该基因在Cu胁迫条件的表达模式进行分析，以期探索黄瓜耐铜(Cu)的分子机制。结果表明：该基因cDNA全长为2 121 bp，编码706个氨基酸；理论分子量为78.36 kDa，等电点为7.76。NCBI Blastp 分析与进化树分析均表明，CsLecRK编码的蛋白与甜瓜的LecRK序列同源性最高(95%)，进化距离最近。qRT-PCR分析表明，CsLecRK在Cu胁迫条件下表达明显下调。
- Abstract:
- Cucumber was used as test material.The mechanism of copper (Cu) tolerance in cucumber was investigated,a candidate Cu tolerance related gene,named lectin receptor kinase (CsLecRK), was cloned and analyzed by using gene cloning and bioinformatics methods based on expression pattern and conservative of genes response to Cu.The results showed that full-length cDNA of CsLecRK was 2 121 bp and encoded a protein of 706 amino acids with predicted molecular mass of 78.36 kDa and a pI of 7.76.Both the results of NCBI Blastp and phylogenetic tree based on LecRK proteins showed that CsLecRK protein had the highest homology with that of muskmelon (95%),the evolutionary distance was the shortest.The result of qRT-PCR showed that CsLecRK was significantly down-regulated under Cu stress.
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备注/Memo
第一作者简介：赵金凤(1991-)，女，河南平顶山人，硕士研究生，研究方向为蔬菜作物营养及抗逆分子生物学。E-mail：1319441066@qq.com.责任作者：武涛(1981-)，男，博士，副教授，现主要从事黄瓜分子遗传育种与蔬菜作物营养及抗逆分子生物学等研究工作。E-mail：wutao@neau.edu.cn.基金项目：黑龙江省普通本科高等学校青年创新人才培养计划资助项目（UNPYSCT-201500）；东北农业大学“青年才俊”资助项目（14QC07）；东北农业大学“学术骨干”资助项目（16XG05）。