[1]郭芳,刘海鹏,李保国,等.光质对红树莓组培苗生长及部分生理生化特性的影响[J].北方园艺,2016,40(22):15-19.[doi:10.11937/bfyy.201622004]
　GUO Fang,LIU Haipeng,LI Baoguo,et al.Effect of Light Quality on Growth and Partial Physiological and Biochemical Characteristics of Red Raspberry Tissue Culture Plantlets[J].Northern Horticulture,2016,40(22):15-19.[doi:10.11937/bfyy.201622004]

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光质对红树莓组培苗生长及部分生理生化特性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷期数: 2016年22 页码: 15-19 栏目: 出版日期: 2016-11-30

Title:: Effect of Light Quality on Growth and Partial Physiological and Biochemical Characteristics of Red Raspberry Tissue Culture Plantlets

作者:: 郭芳1; 2; 刘海鹏1; 2; 李保国1; 2; 张雪梅1; 2; 齐国辉1; 2; 李迎超3; (1.河北农业大学林学院，河北保定 071000；2.河北省林木种质资源与森林保护重点实验室，河北保定 071000；3.中国林业科学研究院林业研究所，国家林业局林木培育重点实验室，北京 100091)

Author(s):: GUO Fang1; 2; LIU Haipeng1; 2; LI Baoguo1; 2; ZHANG Xuemei1; 2; QI Guohui1; 2; LI Yingchao3; (1.College of Forestry，Agricultural University of Hebei，Baoding，Hebei 071000；2.Key Laboratory of Forest Resources and Forest Conservation in Hebei Province，Baoding，Hebei 071000；3.Forestry Research Institute,Chinese Academy of Forestry Sciences/Key Laboratory of Forest Cultivation of State Forestry Bureau，Beijing 100091)

关键词:: 光质; 红树莓; 组培苗; 生长

Keywords:: light quality; red raspberry; tissue culture plantlets; growth

DOI:: 10.11937/bfyy.201622004

文献标志码:: A

摘要:: 以红树莓‘海尔特兹’继代组培苗为试材，研究了不同光质处理下红树莓生根组培苗的形态、抗氧化酶活性以及碳氮代谢，以确立红树莓组培生根苗生长的适宜光质。结果表明：随着R/B（红蓝比)的增加，红树莓生根组培苗的株高由1514 cm逐渐增加到最大值1638 cm，在4R/B（红∶蓝=4∶1)处理下表现为最大值，但与对照W（白光)之间差异不显著，随后株高又随着R/B的增加而减小，在10R/B（红∶蓝=10∶1)处理下表现为最小值0681 cm，显著小于对照W；茎粗则随着R/B的增加由最大值0101 cm逐渐减小到最小值0076 cm，R/4B（红∶蓝=1∶4)处理下表现为最大值，与对照W之间差异显著，10R/B处理下表现为最小值，与对照W之间差异不显著；叶面积、生根率以及根长与茎粗呈现相同的变化趋势，均随着R/B的增加而减小，在R/4B处理下表现为最大值，分别为0751 cm2、0786、1369 cm，在10R/B处理下表现为最小值，分别为0211 cm2、0357、0369 cm；SOD及CAT活性均随着R/B的增加逐渐由R/4B处理下的最小值(1832 μg?g-1FW、H2O2 5902 mg?g-1FW)增加到10R/B处理下的最大值(10953 μg?g-1FW、H2O2 46978 mg?g-1FW)；POD活性随着R/B的增加先由R/4B处理下的最小值(5511 μg?g-1FW)逐渐增加到4R/B处理下的最大值(20934 μg?g-1FW)，随后又随着R/B的增加逐渐减小；MDA含量随着R/B的增加逐渐由R/4B处理下的最小值(7357 nmol?g-1FW)逐渐增加到10R/B处理下的最大值(10880 nmol?g-1FW)，极显著高于对照W；碳水化合物含量随着R/B的增加由200204 mg?g-1增加到最大值313917 mg?g-1，在R/B处理下表现为最大值，且极显著高于对照W，随后又随着R/B的增加逐渐减小到最小值154172 mg?g-1，显著低于对照W；游离氨基酸含量随着R/B的增加逐渐由R/4B处理下的最小值(16903 mg?g-1)增加到10R/B处理下的最大值(33180 mg?g-1)；可溶性蛋白质含量随着R/B的增加逐渐由R/4B处理下的最大值(12507 mg?g-1)减小到10R/B处理下的最小值(2147 mg?g-1)。

Abstract:: In order to establish suitable light quality for the growth of red raspberry tissue culture plantlets，using red raspberry ‘Heritage’ tissue culture plantlets as experimental material，the morphology，anntioxidant enzyme activity，carbon and nitrogen metabolism were studied.The results showed that，with the increasing of R/B(ratio of red to blue)，red raspberry tissue culture plantlet′s height gradually increased from 1514 cm to a maximum of 1638 cm，it was the maximum value at 4R/B(red∶blue=4∶1)，but the difference was not significant with W(white)，then the height of the plant decreased with the increase of R/B，the minimum value was 0681 cm at 10R/B(red∶blue=10∶1)，significantly less than the W.Stem diameter from a maximum of 0101 cm reduced to the minimum value of 0076 cm with the increase of R/B，it was the maximum value at R/4B(red∶blue=1∶4)，the difference was significant with W，it was the minimum value at 10R/B.Leaf area，rooting rate and root length showed the same trend with stem diameter，all decreased with the increase of R/B，it was the maximum value at R/4B，was 0751 cm2，0786，1369 cm，it was the minimum value at 10R/B,was 0211 cm2，0357,0369 cm；SOD and CAT activity increased gradually from the minimum value(1832 μg?g-1FW，H2O25902 mg?g-1FW) of R/4B to the maximum value(10953 μg?g-1FW，H2O2 46978 mg?g-1FW) of 10R/B with the increase of R/B;POD activity increased gradually from the minimum value(5511 μg?g-1FW) of R/4B to the maximum value(20934 μg?g-1FW) of 4R/B，and then gradually decreased with the increase of R/B；MDA content increased gradually from the minimum value(7357 nmol?g-1FW) of R/4B to the maximum value(10880 nmol?g-1FW) of 10R/B with the increase of R/B.Carbohydrate content increased gradually from 200204 mg?g-1 to the maximum value(313917 mg?g-1)，it was the maximum value at R/B and very significantly higher than W，then gradually decreased with the increasing of R/B，the minimum value was 154172 mg?g-1 at 10R/B，significantly lower than W.The content of free amino acid increased gradually from the minimum value(16903 mg?g-1) of R/4B to the maximum value(33180 mg?g-1) of 10R/B with the increase of R/B.Soluble protein content gradually decreased from the maximum value(12507 mg?g-1) of R/4B to the minimum value(2147 mg?g-1) of 10R/B with the increase of R/B．

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备注/Memo

第一作者简介：郭芳（1990-)，女，河北卢龙人，硕士研究生，研究方向为经济林栽培生理。E-mail:guofang901003@163.com．责任作者：李保国（1958-)，男，河北武邑人，博士，教授，博士生导师，现主要从事经济林栽培生理与山区开发技术研究及经济林栽培教学等工作。E-mail：lbg888@163.com．基金项目：河北省“十二五”科技支撑资助项目(16226806D)；国家现〖JP5〗代农业科技城成果惠民科技示范工程资助项目(Z141100002314009)。

更新日期/Last Update: 2017-01-06