MA Li,HAO Zhaoxiang,ZHOU Yuliang,et al.Analysis of Genetic Diversity of Fruit Pomegranate(Punica granatum L.) Cultivars by ISSR Markers[J].Northern Horticulture,2016,40(21):106-110.[doi:10.11937/bfyy.201621027]
果用石榴品种的ISSR遗传多样性分析
- Title:
- Analysis of Genetic Diversity of Fruit Pomegranate(Punica granatum L.) Cultivars by ISSR Markers
- 文献标志码:
- A
- 摘要:
- 果用石榴果实营养丰富，具有鲜食和加工价值。以65个果用石榴品种为试材，采用ISSR标记分析果用石榴类群品种的亲缘关系和遗传多样性。结果表明:10条ISSR引物对65个石榴品种进行PCR扩增，共扩增出186个位点，其中161个是多态性位点，多态性位点比率为（PPB）86.56%，等位基因数（Na）为1.865 6，有效等位基因数（Ne）为1.311 9，Nei′s 的基因多样性指数（He）为0.209 2，Shannon信息指数（I）为0.338 9，品种间遗传相似系数（Gs）介于0.478～0.995，65个果用石榴品种的遗传多样性丰富。UPGMA聚类在GS为0.78时，65个石榴品种分为三大类。聚类结果表明，果用品种间遗传关系与地理环境、籽粒硬度性状有相关性，与果皮颜色、果实风味无相关性。
- Abstract:
- Fruits of the pomegranate have rich nutrition and can be eaten fresh and processed.The genetic relationships and genetic diversity of 65 fruit cultivars of the pomegranate were analyzed by ISSR markers.A total of 186 bands were amplified from 10 ISSR primers，of which 161 were polymorphic loci.The main indexes were PPB 86.56%，Na 1.865 6，Ne 1.311 9，He 0.209 2，I 0.338 9.The genetic similarities coefficient between cultivars ranged from 0.478 to 0.995.65 pomegranate cultivars had abundant genetic diversity.65 pomegranate cultivars were divided into 3 groups at GS 0.78 based on UPGMA method.The results of UPGMA cluster were relevant to geographical origin and seed hardness.The cluster results were not relevant to the peel color and flavor.
参考文献/References:
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备注/Memo
第一作者简介：马丽（1980-），女，博士，副教授，研究方向为植物分子生物学与基因工程。E-mail:mary1976816@163.com.责任作者：侯乐峰（1962-），男，本科，研究员，研究方向为石榴种质资源收集及保存与创新利用。E-mail：houlefeng@126.com.基金项目：山东省高等学校科技计划资助项目（J14LF02）；国家林业局林木种苗工程资助项目（[2012]1888号）；枣庄学院校级基金预研究资助项目（2015YY01）；国家级大学生创新创业训练计划资助项目（201510904028）。