LI Jintong,WU Yani,DING Bing,et al.Establishment of Plant Regeneration System for Four Chrysanthemum morifolium[J].Northern Horticulture,2016,40(19):119-124.[doi:10.11937/bfyy.201619030]
四种露地菊再生体系的建立
- Title:
- Establishment of Plant Regeneration System for Four Chrysanthemum morifolium
- 关键词:
- 露地菊; 再生体系; 6-苄氨基嘌呤(6-BA); 萘乙酸(NAA)
- Keywords:
- Chrysanthemum morifolium; regeneration system; 6-BA; NAA
- 文献标志码:
- A
- 摘要:
- 以‘东林瑞雪’‘夺目玛瑙’‘黄金盏’‘金不换’4种露地菊叶片外植体为试验材料，在MS基本培养基上添加不同浓度的6-BA、NAA进行高效稳定的再生体系的建立。结果表明：‘东林瑞雪’叶片最适分化培养基为MS+0.5 mg?L-1 6-BA+1.5 mg?L-1 NAA；‘夺目玛瑙’叶片最适分化培养基为MS+1.0 mg?L-1 6-BA+2.0 mg?L-1 NAA；‘黄金盏’叶片最适分化培养基为MS+0.5 mg?L-1 6-BA+1.0 mg?L-1 NAA；‘金不换’叶片最适分化培养基为MS+1.0 mg?L-1 6-BA+1.5 mg?L-1 NAA；4种露地菊的最适生根培养基均为1/2MS培养基。
- Abstract:
- This experiment was conducted to construct a high efficient and stable regeneration system by adding different concentration of 6-BA and NAA to the basic MS medium with the leafs explant of four kinds of Chrysanthemum morifolium，including ‘Donglinruixue’ ‘Duomumanao’ ‘Huangjinzhan’ and ‘Jinbuhuan’.The results showed that the optimum concentration combination of NAA and 6-BA for ‘Donlinruixue’ was MS+0.5 mg?L-1 NAA+1.5 mg?L-1 6-BA；for‘Duomumanao’ was MS+1.0 mg?L-1 6-BA+2.0 mg?L-1NAA;‘Huangjinzhan’was MS+0.5 mg?L-1 6-BA+1.0 mg?L-1 NAA；‘Jinbuhuan’ was MS+1.0 mg?L-1 6-BA+1.5 mg?L-1 NAA；and the rooting medium of all the four kinds of Chrysanthemum morifolium was 1/2 MS medium.
参考文献/References:
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备注/Memo
第一作者简介：李金童（1993-），女，硕士研究生，研究方向为植物转基因。E-mail：1552066612@qq.com.责任作者：解莉楠（1978-），女，博士，副教授，研究方向为植物抗逆性生物学。E-mail：linanxie@126.com.基金项目：“十二五”农村领域国家科技计划资助项目(2013AA102706)。