ZHANG Lifang,WANG Jinghua,SHI Guoxin,et al.The Oxidative Damage of Spirodela polyrrhiza Under Mn Stress[J].Northern Horticulture,2016,40(19):89-96.[doi:10.11937/bfyy.201619024]
锰（Mn）胁迫对紫萍的氧化损伤
- Title:
- The Oxidative Damage of Spirodela polyrrhiza Under Mn Stress
- 关键词:
- 锰（Mn）; 紫萍; 氧化损伤; 傅里叶变换红外光谱分析
- 文献标志码:
- A
- 摘要:
- 研究了不同浓度Mn胁迫(0、0.02、0.04、0.06、0.08、0.10 mmol?L-1)对紫背浮萍的氧化损伤、抗氧化系统以及Ca2+-ATP酶活性变化的影响，并应用傅里叶变换红外光谱（FTIR）分析了紫萍红外光谱图的变化。结果表明：Mn胁迫对紫萍造成了明显的氧化损伤，O?[TX-*4]2产生速率明显上升、H2O2和MDA大量积累，光合色素、可溶性蛋白质含量下降，可溶性糖含量短暂升高后下降。抗氧化系统遭到了破坏，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性先升高后降低，过氧化物酶（POD）活性逐渐升高，谷光甘肽（GSH）、维生素C及维生素E含量明显上升。脯氨酸含量明显上升，Ca2+-ATP酶活性先升高后明显下降。红外光谱图显示，在3 396、2 920、1 658、1 062 cm-1处峰值均先上升后下降，反映出Mn胁迫下紫萍体内氨基酸、糖类、蛋白质、羧酸等物质的含量也受到显著的影响。综上可见，Mn处理下，紫萍受到明显的氧化损伤，抗氧化系统的平衡被打破，Ca2+-ATP酶活性的变化明显，氨基酸、糖类、羧酸等物质的含量也受到明显的影响，即Mn胁迫对紫萍造成了显著伤害。
- Abstract:
- The effect of Mn (0，0.02，0.04，0.06，0.08，0.10 mmol?L-1) in different concentrations in the Spirodela polyrrhiza was studied，and the oxidative damage，antioxidant system and the influence of Ca2+-ATP enzyme activity in the S.polyrrhiza were analyzed.The change of the infrared spectra was analyzed by Fourier transform infrared spectroscopy (FTIR).The results showed that Mn stress caused obvious oxidative damage,such us the dramatical increase of the generation rate of O?[TX-*4]2，the accumulation of H2O2 and MDA in great quantities，and the obvious decreases of the content of soluble protein and photosynthetic pigment in leaves.The content of the soluble sugar decreased after an increase in short.The antioxidant system was destroyed.Along with the increase of Mn concentration，the activity of catalase (CAT) and superoxide dismutase (SOD) declined after increasing.But the activity of peroxidase (POD) in leaves increased gradually，and the levels of GSH，vitamin C and vitamin E increased sharply.The content of proline increased obviously.The activity of the Ca2+-ATP enzyme obviously decreased after increasing.The infrared spectra showed that the peak increased firstly and then decreased in the place of 3 396 cm-1，2 920 cm-1，1 658 cm-1 and 1 062 cm-1,which reflected the content of amino acid，sugars，proteins and carboxylic acid were obviously effected by the Mn stress.In a conclusion，oxidative damage of the S.polyrrhiza was obvious under Mn treatment.The balance of antioxidant system has been broken.The activity of the Ca2+-ATP enzyme changed obviously.In addition，the content of amino acids，sugars，carboxylic acid were also significantly affected.In other words，S.polyrrhiza was obviously damaged by the Mn stress.
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备注/Memo
第一作者简介：张立芳（1988-），女，硕士研究生，研究方向为水体重金属污染的植物毒害和植物修复。E-mail：zlfwky2015@163.com.责任作者：施国新（1950-），男，教授，博士生导师，现主要从事水体重金属污染的植物毒害和植物修复教学与科研等工作。E-mail：gxshi@ njnu.edu.cn.基金项目：江苏高校优秀学科建设工程资助项目（164320H106）。