WANG Dianzhen,HE Xiaolong,ZHAO Ruihua,et al.Comparison of Cordyceps militaris Spawn Rejuvenation Method[J].Northern Horticulture,2016,40(17):137-139.[doi:10.11937/bfyy.201617032]
北虫草菌种复壮方法的比较
- Title:
- Comparison of Cordyceps militaris Spawn Rejuvenation Method
- Keywords:
- Cordyceps militaris; culture; rejuvenation
- 文献标志码:
- A
- 摘要:
- 以已退化的北虫草菌种为试材，采用孢子分离、组织分离、活体蚕蛹、基内菌丝分离、蚕蛹回接等5种菌种复壮法，研究了不同复壮方法对退化菌种复壮效果的影响。结果表明：经活体蚕蛹复壮法得到的菌种在菌丝生长速度和子实体产量均是最高的，菌丝生长速度提高了29.97%，子实体产量提高了383.93%，故确定活体蚕蛹复壮法是5种方法中最好的虫草菌种复壮法。
- Abstract:
- Taking Cordyceps militars as test materials，the effects of five rejuvenation methods,including the spore separation and rejuvenation,tissue isolation and rejuvenation,living silkworm rejuvenation,substrate mycelium separation and rejuvenation and silkworm chrysalis tieback rejuvenation on the rejuvenation of degraded Cordyceps strains were studied. The results showed that through live silkworm pupa rejuvenation of the strains in hyphal growth speed and yield of fruitbody were the highest，mycelial growth rate was increased 29.97%，fruitbody yield was increased 383.93%.So the living silkworm rejuvenation method was the best method of Cordyceps militaris in 5 kinds of methods.
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备注/Memo
第一作者简介：王殿振（1989-）,男,河南濮阳人,硕士研究生,研究方向为应用微生物及食用真菌学。E-mail:340680760@qq.com.责任作者：贺晓龙（1982-）,男，陕西临潼人,硕士研究生,实验师,现主要从事生物技术与食用菌等研究工作。E-mail：254441886@qq.com.基金项目：陕西科技厅协同创新资助项目（2015XT-34）；陕西省教育厅科研资助项目（15JK1815）；延安大学研究生教育创新资助项目。