FENG Rong,PANG Kejian,TANG Hui,et al.Comparison of Content of Flavonoid in Diaphragma Kernel Pellicle and Shell of Juglans regia From Different Areas[J].Northern Horticulture,2016,40(16):27-30.[doi:10.11937/bfyy.201616006]
不同产地核桃隔膜、种皮及壳中黄酮含量的比较
- Title:
- Comparison of Content of Flavonoid in Diaphragma Kernel Pellicle and Shell of Juglans regia From Different Areas
- Keywords:
- shell of Juglans regia; diaphragma of Juglans regia; kernel pellicle of Juglans regia; Luteolin-7-O-β-D-glucoside; flavonoid
- 文献标志码:
- A
- 摘要:
- 采用超声提取法提取不同产地核桃不同部位总黄酮，以木犀草苷为对照品，采用HPLC法和比色法测定提取物中木犀草苷和总黄酮含量，比较不同产地核桃隔膜、种皮及壳中木犀草苷及总黄酮的含量差异，为药用核桃的道地性及药用部位提供依据。结果表明：不同产地核桃中木犀草苷含量为长治>和田>阿克苏>塔里木>大理>秦岭，总黄酮含量为大理>秦岭>和田>塔里木>长治>阿克苏。核桃不同组织部位中木犀草苷的分布为种皮>壳>隔膜，不同组织部位中总黄酮的分布为壳>种皮>隔膜。表明，不同产地核桃的黄酮成分含量有差别，山西核桃木犀草苷含量最高，但总黄酮含量最低；云南核桃总黄酮含量最高，但木犀草苷含量很低。核桃种皮中木犀草苷及总黄酮含量要高于隔膜中的，核桃种皮可能和隔膜有相似的药理活性，同样具有很好的药用价值。
- Abstract:
- To compare the difference in content of flavonoids in diaphragma kernel pellicle and shell of Juglans regia from different regions，and determine the content of Luteolin-7-O-β-D-glucoside in three different parts of walnut.HPLC was used to establish content of Luteolin-7-O-β-D-glucoside in three extracts，and colorimetry were used to compare the content of total flavonoids in the samples.The results showed that，the content of Luteolin-7-O-β-D-glucoside in different producing area of walnut was Changzhi>Hotan>Akesu>Tarim>Dali>Qinling，the content of total flavonoids：Dali>Qinling>Hotan>Tarim>Changzhi>Akesu.The content of Luteolin-7-O-β-D-glucoside in different parts of walnut：kernel pellicle>shell>diaphragma.The content of total flavonoids：shell>kernel pellicle>diaphragma.There were some differences of contents of total flavonoids in different regions，the walnuts of Shanxi content of Luteolin-7-O-β-D-glucoside was generally higher than other regions，but the content of total flavonoids was the least.The walnuts of Yunnan content of total flavonoids was higher than other regions，but the Luteolin-7-O-β-D-glucoside was less.Kernel pellicle might had similar pharmacological activity as diaphragma of Juglans regia，because it contained higher flavonoids and had good medicinal value as well.
参考文献/References:
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备注/Memo
第一作者简介：冯荣（1991-），女，硕士研究生，研究方向为药物分析学。E-mail：409007049@qq.com.责任作者：唐辉（1967-），女，博士，教授，研究方向为药物分析及新药研究。E-mail：th_pha@shzu.edu.cn.基金项目：国家科技支疆计划资助项目（2008ZJ14）。