[1]钱双,李昂,赵鑫,等.Chrysanthemum weyrichii基因组测定及rDNA定位[J].北方园艺,2016,40(12):101-105.[doi:10.11937/bfyy.201612025]
　QIAN Shuang,LI Ang,ZHAO Xin,et al.Estimation of Genomic Size and Physical Location of rDNA of Chrysanthemum weyrichii[J].Northern Horticulture,2016,40(12):101-105.[doi:10.11937/bfyy.201612025]
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Chrysanthemum weyrichii基因组测定及rDNA定位

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年12 页码: 101-105 栏目: 出版日期: 2016-06-30
Title:
Estimation of Genomic Size and Physical Location of rDNA of  Chrysanthemum weyrichii
作者:
钱双1; 2; 李昂2; 赵鑫2; 杨树华2; 李名扬1; 葛红2
（1.西南大学 园艺园林学院，重庆市花卉工程技术研究中心，重庆 400716；2.中国农业科学院 蔬菜花卉研究所，农业部园艺作物生物学与种质创制重点实验室，国家花卉改良中心，北京 100081）
Author(s):
QIAN Shuang1; 2; LI Ang2; ZHAO Xin2; YANG Shuhua2; LI Mingyang1; GE Hong2
(1.College of Horticulture and Landscape Architecture/Chongqing Engineering Research Center for Floriculture,Southwest University，Chongqing 400716；2.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Ministry of Agriculture/Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences/National Flowers Improvement Center of China,Beijing 100081)
关键词:
Chrysanthemum weyrichii; 流式细胞术; 染色体; rDNA; 荧光原位杂交
Keywords:
Chrysanthemum weyrichii; flow cytometry; chromosome; rDNA; fluorescence in situ hybridization
DOI:
10.11937/bfyy.201612025
文献标志码:
A
摘要:
以Chrysanthemum weyrichii 为试材，采用流式细胞术估算其基因组大小，同时利用染色体计数法统计了C.weyrichii的染色体条数并对其rDNA染色体定位进行了研究。结果表明：C.weyrichii基因组大小为10.02 Gb，染色条数为2n=8x=72，5S rDNA杂交信号有4对位点，位于染色体的亚中部；45S rDNA杂交信号有8对位点，位于染色体的末端。该研究结果为该物种基因组分析以及菊属植物染色体进化研究提供了依据。
Abstract:
Taking Chrysanthemum weyrichii as test material,the genome size of C.weyrichii was estimated by flow cytometry.The chromosome numbers and rDNA chromosomal location of C.weyrichii were studied by chromosome counting method and fluorescence in situ hybridization for the first time.The results showed that the genome size was 10.02 Gb,chromosome numbers were 2n=8x=72,four pairs of 5S rDNA located at submetacentric regions and eight pairs of 45S rDNA located at terminal regions were detected.This study provided a valuable basis for future genomic and cytogenetic studies in C.weyrichii.

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备注/Memo

第一作者简介：钱双（1991-），女，四川广安人，硕士研究生，研究方向为观赏植物生物技术与遗传育种。E-mail：990602213@qq.com.责任作者：葛红（1964-），女，研究员，研究方向为花卉资源的收集与育种。E-mail：gehong@caas.cn.基金项目：国家自然科学基金资助项目（C150302）；国家‘863’计划资助项目（2011AA100208）。

更新日期/Last Update: 2016-07-21