[1]刘敏,卢红,黄媛媛,等.茶树菇液体发酵条件研究[J].北方园艺,2016,40(11):142-144.[doi:10.11937/bfyy.201611037]
　LIU Min,LU Hong,HUANG Yuanyuan,et al.Study on the Liquid Fermentation Condition of Agrocybe aegerita(Brig.) Sing[J].Northern Horticulture,2016,40(11):142-144.[doi:10.11937/bfyy.201611037]
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茶树菇液体发酵条件研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年11 页码: 142-144 栏目: 出版日期: 2016-05-15
Title:
Study on the Liquid Fermentation Condition of Agrocybe aegerita(Brig.) Sing
作者:
刘敏1; 卢红2; 黄媛媛2; 荣群2; 王谦1
（1.河北大学 生物技术研究中心，河北 保定 071002；2.河北大学 生命科学学院，河北 保定 071002）
Author(s):
LIU Min1; LU Hong2; HUANG Yuanyuan2; RONG Qun2; WANG Qian1
（1.Research Center of Biotechnology Engineering，Hebei University，Baoding,Hebei 071002；2.College of Life Science，Hebei University,Baoding,Hebei 071002）
关键词:
茶树菇; 液体培养基; 培养条件; 优化; 菌丝体鲜重
Keywords:
Agrocybe aegerita(Brig.) Sing; culture medium; culture condition; optimization; mycelium fresh weight
DOI:
10.11937/bfyy.201611037
文献标志码:
A
摘要:
以茶树菇为试材，采用液体摇瓶培养法，通过单因素试验、正交实验，以菌丝体生物量为主要指标，对茶树菇液体发酵培养基配方及培养条件进行优化。结果表明：最适宜的培养基配方为葡萄糖3.0%、豆饼粉1.0%、酵母膏0.2%、MgSO4?7H2O 0.10%、KH2PO4 0.10%。适宜的茶树菇液体培养条件为起始pH 6.0~7.0、培养温度25~28 ℃、摇床转速150~180 r?min-1。
Abstract:
Taking Agrocybe aegerita as test material，the liquid fermentation condition of Agrocybe aegerita in submerged culture with shake flask were studied according to the index of mycelium fresh weight by single factor experiment and quadrature experiment.The results showed that the optimal liquid culture medium was as follows：glucose 3.0%，soybean cake powder 1.0%，yeast extract 0.2%，MgSO4?7H2O 0.10%，KH2PO4 0.10%.The optimal culture condition were initial pH 6.0-7.0，temperature 25-28 ℃，shaking rate 150-180 r?min-1.

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备注/Memo

第一作者简介：刘敏（1985-），女，硕士，实验师，现主要从事食药用真菌研究与开发等研究工作。E-mail：liumin870115@163.com.基金项目：河北省现代农业产业技术体系食用菌创新团队资助项目。

更新日期/Last Update: 2016-07-15