REN Xibo,YUAN Jincheng,DAI Xiyao,et al.Study on Physiological and Biochemical Characteristics and Genetic Integrity in Cauliflower[J].Northern Horticulture,2016,40(10):19-23.[doi:10.11937/bfyy.201610005]
花椰菜种质资源遗传完整性变化的生理生化特性研究
- Title:
- Study on Physiological and Biochemical Characteristics and Genetic Integrity in Cauliflower
- Keywords:
- cauliflower; aging treatment; genetic integrity; physiological indexes; biochemical indicators
- 文献标志码:
- A
- 摘要:
- 为了找到适合表现花椰菜种质遗传完整性的生理生化指标，为作物种质资源的收集和保存工作提供依据。以3个品种的花椰菜种质为试材，研究了经老化处理后种子的发芽率、发芽势、过氧化物酶（POD）活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、可溶性糖含量和电导率值的变化。结果表明：随着老化处理时间的加长，种子发芽率和发芽势、过氧化物酶、超氧化物歧化酶活性和丙二醛含量均呈下降状态，可溶性糖含量以及电导率值显著增加。由相关性分析可知，不同品种最适的遗传完整性评价指标不同，POD活性、SOD活性、电导率值和可溶性糖含量能够作为花椰菜品种遗传完整性的评价指标。
- Abstract:
- In order to find the cauliflower genetic integrity of physiological and biochemical change rule,to provide the basis for crop germless collection and preservation work.With three varieties of cauliflower as test materials,after aging treatment was conducted to study the seed germination rate，germination potential，hydrogen peroxide enzyme (POD)，super oxide dismutase (SOD) activity，malondialdehyde (MDA) content，soluble sugar content and conductivity value changes.The results showed that seed germination rate and germination energy were significantly decreased；POD activity，SOD activity and MDA decreased，a significant increase in soluble sugar content and conductivity value as the lengthen of aging treatment time.By the correlation analysis，different kind of genetic integrity of the optimal evaluation，POD activity，SOD activity，soluble sugar content and conductivity value could be used as the genetic integrity of cauliflower evaluation.
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备注/Memo
第一作者简介：任喜波（1978-），女，博士，副教授，研究方向为蔬菜遗传育种。E-mail：renxibo@163.com. 基金项目：河北省科学技术研究与发展计划资助项目（11220162）；张家口市科学技术研究与发展计划资助项目（1012005C，1112012C）；河北北方学院创新团队资助项目（CXTD1307）；河北北方学院重大资助项目（ZD201309）；河北北方学院重点试验生命科学研究中心资助项目。