LIU Hongwei,ZHANG Rongrong,LIU Yanling,et al.Effect of Nitrogen on Pennisetum sp.Seeding Stage Growth and Photosynthetic Physiological Characteristics[J].Northern Horticulture,2016,40(08):133-138.[doi:10.11937/bfyy.201608037]
氮素对巨菌草苗期生长和光合生理特性的影响
- Title:
- Effect of Nitrogen on Pennisetum sp.Seeding Stage Growth and Photosynthetic Physiological Characteristics
- Keywords:
- Pennisetum sp.; nitrogen levels; growth characteristics; photosynthesis; osmotic adjustment; membrane lipid peroxidation
- 文献标志码:
- A
- 摘要:
- 以巨菌草为试材，采用水培方法，研究了不同浓度的氮素对巨菌草苗期生长和光合生理特性的影响。结果表明：随着氮素浓度的增加，株高、根总长、根表面积、根体积、分根数、根尖数呈先升后降的趋势，叶片数、叶面积、茎粗则呈上升趋势；净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）先增大后减小，胞间CO2浓度（Ci）则逐渐降低；光合色素、可溶性蛋白质、可溶性糖、脯氨酸含量，过氧化物酶（POD）、超氧化物歧化酶（SOD）活性呈先升后降的趋势，硝态氮（NO-3-N）含量、过氧化氢酶（CAT）活性呈上升趋势，丙二醛（MDA）含量呈下降趋势。研究表明，适量的氮素供应（5.00～10.00 mmol/L）可以促进植株生长，提高光合能力，增加渗透调节物质含量、减少MDA含量及提高抗氧化物酶活性，有利于植株生长并增强了其抵抗逆境胁迫的能力，氮素供应不足（0.00～0.50 mmol/L）或过量（15.00 mmol/L）均不同程度限制了植株的正常生长及生理代谢活动。
- Abstract:
- Taking Pennisetum sp.as test material，hydroponic experiment was carried out to study the response of different concentration of nitrogen (N) on seeding stage growth and photosynthetic physiological characteristics of Pennisetum sp..The results showed that with the improvement of N concentration，plant height，total root length，root surface area，root volume，number of root forks，number of root tips all increased first and then decreased，leaf number，leaf area and stem diameter decreased；Pn，Tr and Gs rose and then dropped,Ci continuously dropped；the contents of photosynthetic pigment，soluble protein，soluble sugar，proline，and the activities of POD and SOD all showed a trend of first increasing and then decreasing，the content of NO3--N and the activity of CAT increased，while the content of MDA decreased.The study indicated that optimal N supply(5.00-10.00 mmol/L)could promote plant shoot and root growth，enhance photosynthesis，raise the contents of osmotic adjustable substances，reduce the content of MDA，and promote the activities of antioxidant enzymes，which was in favor of the growth of plant and strengthened their adaptability to the adverse environments.The growth and physiological metabolism activity was limited in different extent when the N supply was in short(0.00-0.50 mmol/L)or excessive(15.00 mmol/L)conditions.
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备注/Memo
第一作者简介：刘宏伟（1988-），男，硕士研究生，研究方向为菌草生态治理。E-mail：liuhongwei11433@163.com. 责任作者：林占熺（1943-），男，研究员，博士生导师，现主要从事菌草生态治理等研究工作。基金项目：浑善达克沙地疏林型植被建设技术研究资助项目（201504412）；荒漠化地区菌草治理技术的研究及应用资助项目（JA13096）；福建省菌草生态产业协同创新中心资助项目（K80ND8002）；福建省自然科学基金资助项目（2015J05047）。