MA Yadong,LIU Huiying,ZHANG Xiaoqian,et al.Effect of Different Nitrogen Levels on Growth，Photosynthetic Characteristics and Reactive Oxygen Scavenging Capacity of Cichorium endivia L.[J].Northern Horticulture,2016,40(15):21-26.[doi:10.11937/bfyy.201615005]
供氮水平对苦苣生长、光合特性及活性氧清除能力的影响
- Title:
- Effect of Different Nitrogen Levels on Growth，Photosynthetic Characteristics and Reactive Oxygen Scavenging Capacity of Cichorium endivia L.
- Keywords:
- Cichorium endivia L.; nitrogen level; growth; photosynthetic characteristics; reactive oxygen scavenging capacity
- 文献标志码:
- A
- 摘要:
- 以苦苣为试材，采用营养液栽培法，研究了不同供氮水平对苦苣生长、根系活力、叶片叶绿素相对含量（SPAD值）、光合气体交换参数、H2O2和丙二醛（MDA）含量以及活性氧清除能力的影响。结果表明：在5个不同供氮处理水平中，8 mmol?L-1（N8）的施氮水平下苦苣植株生长最佳，这与植株具有较高水平的根系活力、SPAD值、净光合速率(Pn)和较高的活性氧清除能力、较低的膜脂过氧化程度有关。而低氮（2、5 mmol?L-1）或高氮供应水平（11、14 mmol?L-1）因不同程度降低苦苣植株根系活力、叶片光合色素含量和活性氧清除能力，导致H2O2过量积累、膜脂过氧化加剧，细胞膜的结构及功能受损伤，从而抑制苦苣植株的光合作用和生长。
- Abstract:
- With Cichorium endivia L.as test material，hydroponic experiments were conducted to investigate the effects of different nitrogen levels on plant growth and root activity，as well as chlorophyll relative content (SPAD value)，photosynthetic gas exchange parameters，the contents of H2O2 and malondialdehyde (MDA) and active oxygen scavenging capacity in leaves of endive.The results showed that，the growth of endive plants under 8 mmol?L-1(N8) nitrogen level treatment was the best among of five different nitrogen treatments，which was related to the higher root activity，SPAD value，net photosynthetic rate (Pn) and reactive oxygen species scavenging capability，as well as lower membrane lipid peroxidation in endive plants.Furthermore，the application of low nitrogen (2 mmol?L-1,5 mmol?L-1) or high nitrogen level (11 mmol?L-1,14 mmol?L-1) decreased Pn and inhibited the growth of endive plants in different degree likely due to the decrease in root activity and leaf photosynthetic pigment content and the damage in structure and function of cell membrane caused by the decrease of active oxygen scavenging capacity，which resulted in the accumulation of H2O2 and membrane lipid peroxidation enhancement.
参考文献/References:
[1]徐燕,梁敬钰.苦苣菜的化学成分[J].中国药科大学学报,2005,36(5):411-413. [2]马祥忠,曾阳,吴旸,等.苦苣菜属植物的化学成分及药理作用研究进展[J].青海师范大学学报(自然科学版),2011(2):49-52. [3]SHARMA S N.Effects of ammonium and nitrate on CO2 assimilation,RuBPC,PEPC and dry matter production[J].Res,1987,12(3):265-272. [4]蒋达波,宗秀虹,李帮秀,等.氮素胁迫对玉米光合及叶绿素荧光参数的影响[J].西南师范大学学报(自然科学版),2015,40(1):135-139. [5]陈修斌,李翊华,许耀照.温室番茄对水肥耦合的光合特性及叶绿素荧光参数响应[J].土壤通报,2015,46(3):687-693. [6]马宗斌,严根土,刘桂珍,等.施氮量对黄河滩区棉花叶片生理特性、干物质积累及产量的影响[J].植物营养与肥料学报,2013,19(4):849-857. [7]赵长星,马东辉,王月福,等.施氮量和花后土壤含水量对小麦旗叶衰老及粒重的影响[J].应用生态学报,2008,19(11):2388-2393. [8]PATTERSON B D，MACRAE E A，FERGUSON I B.Estimation of hydrogen peroxide in plants extracts using titanium(IV)[J].Annals of Biochem,1984,134:487-492. [9]DRAPER H H，HADLEY M.Malondialdehyde determination as index of lipid peroxidation[J].Method Enzymol,1990,186:421-431. [10]RAO K V M,SRESTY T V S.Antioxidant parameters in the seedlings of pigeon pea (Cajanus cajan L.Mill spaugh) in response to Zn and Ni stresses[J].Plant Sci,2000,157:113-128. [11]ZHANG X Z.The measurement and mechanism of lipid peroxidation and SOD,POD and CAT activities in biological system[M]//ZHANG X Z(Ed.).Research methodology of crop physiology.Beijing:Agriculture Press,1992:208-211. [12]LAW M Y,CHARLES S A，HALLIWELL B.Glutathione and ascorbic acid in spinach(Spinacia oleracra)chloroplasts[J].Biochemical Journal,1983,210:899-903. [13]李俊良,陈新平,李晓林,等.大白菜氮肥施用的产量效应、品质效应和环境效应[J].土壤学报,2003,40(2):261-266. [14]FARQUHAR G D,SHARKEY T D.Stomatal conductance and photosynthesis[J].Annu Rev Plant Physiol,1982,33:317-345. [15]李强,罗延宏,余东海,等.低氮胁迫对耐低氮玉米品种苗期光合及叶绿素荧光特性的影响[J].植物营养与肥料学报,2015,21(5):1132-1141. [16]曾希柏,谢德体,青长乐,等.氮肥施用量对莴笋光合特性影响的研究[J].植物营养与肥料学报，1997,3(4):323-328. [17]DRAPER H H，HADLEY M.Malondialdehyde determination as index of lipid peroxidation[J].Method Enzymol,1990,186:421-431. [18]张英鹏,林咸永,章永松.供氮水平对菠菜营养品质和体内抗氧化酶活性的影响[J].应用生态学报,2005,16(3):519-523. [19]鲜开梅,刘慧英.不同氮素水平对彩椒几项生理指标及活性氧清除系统的影响[J].北方园艺,2015(4):17-21.
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备注/Memo
第一作者简介：马亚东（1992-），男，安徽蒙城人，硕士研究生，研究方向为设施作物栽培生理。E-mail:1595990994@qq.com.责任作者：刁明（1968-），男，新疆吐鲁番人，博士，教授，现主要从事作物模拟模型与精准农业等研究工作。基金项目：国家星火重点资助项目（2012GA8910033，2015GA891008）；国家自然科学基金资助项目（31360478）。