CHEN Xuefeng,WEI Shiyan,WU Shengjin,et al.Genetic Diversity Analysis of Wild Ganoderma Strains Collected From Guangxi[J].Northern Horticulture,2016,40(07):136-139.[doi:10.11937/bfyy.201607034]
广西野生灵芝菌株的遗传多样性分析
- Title:
- Genetic Diversity Analysis of Wild Ganoderma Strains Collected From Guangxi
- Keywords:
- wide Ganoderma spp.; esterase isozyme; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以10个广西野生灵芝菌株为试材，采用酯酶同工酶分析和拮抗试验的方法，研究供试菌株的遗传多样性。结果表明：广西野生灵芝具有丰富的遗传多样性。试验共检测到22条谱带，10个菌株间的谱带均有差异。各菌株间的遗传相似系数在0.36~0.95；在相似系数为0.55时，10个菌株可以分为3类，即菌株C3（红芝)为一类，菌株C9（白芝）为一类，C1、C2、C4、C5、C6、C7、C8、C10（黑芝）为一类；在相似系数为0.64时，黑芝类又可分为2类，即C1、C2、C4、C8、C10为一类，C5、C6、C7为一类。菌株C4与C10、C5与C7间的遗传相似系数大于0.90，亲缘关系很近，且这2对菌株间无拮抗，说明菌株C4与C10为同一菌株，C5与C7也为同一菌株。
- Abstract:
- Genetic diversity of ten wild Ganoderma strains collected from Guangxi was analyzed by using the technique of esterase isozyme analysis and antagonistic experiment.The results showed that there were rich genetic polymorphisms within the wild Ganoderma in Guangxi.22 bands were determined in the trail，and the bands among the strains were different.Cluster analysis demonstrated that the similarity coefficients ranged from 0.36 to 0.95 among the ten strains and were divided into 3 groups at the similarity coefficient of 0.55，which was Ganoderma lingzhi (strain C3)，Ganoderma lucidum（strain C9）and Ganoderma atrum（strains C1，C2，C4，C5，C6，C7，C8，C10）.The group of G.atrum could be divided into 2 subgroups (subgroup A included strains C1，C2，C4，C8 and C10，subgroup B included strains C5，C6 and C7） at the similarity coefficient of 0.64.Strains C4 and C10，as well as strains C5 and C7 could be considered as different isolates in the same strain，as they had high similarity coefficients above of 0.90 and without antagonistic activity between both strains.
参考文献/References:
[1]陈振妮,陈丽新,黄卓忠,等.广西灵芝产业现状及发展建议[J].食用菌,2015(1):4-6. [2]贾定洪,郑林用,张小平,等.灵芝属38个菌株的酯酶同工酶研究[J].西南农业学报,2006，19(3):502-505. [3]唐传红,张劲松,陈明杰,等.灵芝属菌株遗传多样性的初步研究[J].南京农业大学学报 2005，28(2)：133-136. [4]潘丽晶,沈汉国,陈继敏，等.荧光AFLP和拮抗实验对灵芝菌遗传多样性研究[J].中国食用菌,2013,32(6):38-42. [5]韦仕岩,王灿琴,覃小娟,等.金福菇菌株的酯酶同工酶分析[J].基因组学与应用生物学,2014,33(5):1025-1030. [6]顾敏,沈颖越,金群力,等.双孢蘑菇SSR分子标记开发及其在遗传多样性分析中的应用[J].浙江农业学报,2013,25(5):987-993. [7]王华,李渊,郭尚,等.5种食用菌遗传多样性的RAPD分子标记[J].山西农业科学,2015,43(5):516-517,547.
相似文献/References:
[1]李守勉,李 明,田景花.不同培养时间对杏鲍菇酯酶同工酶酶谱的影响[J].北方园艺,2014,38(14):98.
LI Shou-mian,LI Ming,TIAN Jing-hua.Influences of Different Culture Times on Zymogram of Esterase Isozyme of Pleurotus eryngii[J].Northern Horticulture,2014,38(07):98.
[2]刘贵巧,暴增海,薛晓畅.冀南平菇菌种资源鉴定研究[J].北方园艺,2012,36(24):162.
LIU Gui-qiao,BAO Zeng-hai,QUE Xiao-chang.Preliminary Study on Oyster Mushroom Strain Identification in the Southern of Hebei[J].Northern Horticulture,2012,36(07):162.
[3]郑素月,郝俊灵,王建伟.继代培养对蛹虫草菌丝生长速度及酯酶同工酶的影响[J].北方园艺,2012,36(10):136.
ZHENG Su-yue,HAO Jun-ling,WANG Jian-wei.The Influences of Subculture of Cordceps militaris to Mycelial Growth Rate and Esterase Isozyme[J].Northern Horticulture,2012,36(07):136.
[4]崔丹,姚方杰,张友民.金顶侧耳酯酶同工酶多样性的研究[J].北方园艺,2012,36(10):179.
CUI Dan,YAO Fang-jie,ZHANG You-min.Study on Esterase Isozyme Analysis Diversity of Pleurotus Citrinopileatus[J].Northern Horticulture,2012,36(07):179.
[5]王谦,马旭光,刘敏,等.原生质体紫外诱变选育猴头菌株[J].北方园艺,2014,38(16):145.
WANG Qian,MA Xu-guang,LIU Min,et al.Selection for a Hericium erinaceus Mutant Strain in the Way of Protoplasts Being Exposed to the UV Radiation[J].Northern Horticulture,2014,38(07):145.
[6]宋莹,刘娜,肖千明,等.十五个香菇菌株遗传特异性研究[J].北方园艺,2014,38(18):163.
SONG Ying,LIU Na,XIAO Qian-ming,et al.Study on Genetic Specificity of 15 Lentinula edodes Strains[J].Northern Horticulture,2014,38(07):163.
[7]刘丽杰,林立东,张东向.黄芩单细胞克隆愈伤组织酯酶同工酶及过氧化物同工酶酶谱分析[J].北方园艺,2015,39(07):85.[doi:10.11937/bfyy.201507026]
LIU Li-jie,LIN Li-dong,ZHANG Dong-xiang.Analysis on Esterase Isozemes and Peroxidase Isozymes in Singlecell Clone of Scutellaria Callus[J].Northern Horticulture,2015,39(07):85.[doi:10.11937/bfyy.201507026]
[8]高坤梅,邢志伟,郑素月,等.白灵菇杂交子的筛选及鉴定[J].北方园艺,2015,39(09):121.[doi:10.11937/bfyy.201509034]
GAO Kun-mei,XING Zhi-wei,ZHENG Su-yue,et al.Selection and Identification of Hybrid Strain of Pleurotus eryngii var. tuoliensis[J].Northern Horticulture,2015,39(07):121.[doi:10.11937/bfyy.201509034]
[9]刘 贵 巧,米 青 荣,黄 慧 敏.平菇长速、 长相与酯酶同工酶、 可溶性蛋白含量关系的探究[J].北方园艺,2010,34(08):0.[doi:10.11937/bfyy.201008075]
LIU Gui O qiao,MI Qing Orong,HUANG Hui Omin.Research for the Relationship Between Growth Speed, Looks and Esterase Isozyme, Soluble Protein Content in Several Strains of Pleurotusostreat[J].Northern Horticulture,2010,34(07):0.[doi:10.11937/bfyy.201008075]
[10]郑素月,李云梦,邢志伟,等.七个不同鸡腿菇菌株的酯酶同工酶和ISSR分析[J].北方园艺,2015,39(17):85.[doi:10.11937/bfyy.201517022]
ZHENG Suyue,LI Yunmeng,XING Zhiwei,et al.Analysis of Esterase Isozyme and ISSR of Seven Different Coprinus comatus[J].Northern Horticulture,2015,39(07):85.[doi:10.11937/bfyy.201517022]
备注/Memo
第一作者简介：陈雪凤（1976-），女，本科，高级农艺师，现主要从事食用菌育种及推广等工作。E-mai:xuefeng767@126.com. 责任作者：韦仕岩（1965-）,男，硕士，研究员，现主要从事食用菌育种等研究工作。基金项目：广西农业科学院基本科研业务专项资助项目（2015YT77，2015JZ60）；国家现代农业产业技术体系广西食用菌创新团队专项资金资助项目。