YANG Shenming,FAN Shuguo,WANG Zhenji,et al.Ethanol Reflux Method Extraction and Antioxidant Activities of Total Flavonoids From Lycopodii herba[J].Northern Horticulture,2016,40(06):116-120.[doi:10.11937/bfyy.201606029]
乙醇回流法提取伸筋草总黄酮工艺及其体外抗氧化活性
- Title:
- Ethanol Reflux Method Extraction and Antioxidant Activities of Total Flavonoids From Lycopodii herba
- 文献标志码:
- A
- 摘要:
- 以伸筋草为试材,在单因素试验的基础上,以总黄酮提取率为指标,采用正交实验L9(34) 优化乙醇辅助回流提取总黄酮的最佳工艺条件,并通过总黄酮对羟基自由基和1,1-苯基-2-苦肼基自由基的清除能力来评价其抗氧化性。结果表明:最佳提取工艺参数为乙醇体积分数75%,液料比60∶1 mL/g,提取时间150 min,提取温度80℃,在此条件下,总黄酮的平均提取率为1.82%;总黄酮质量浓度为0.014 0 mg/mL时,对?OH和DPPH?的清除率可分别达72.65%和93.02%,说明总黄酮对?OH和DPPH?有较好的清除能力。
- Abstract:
- The optimal ethanol reflux extraction conditions of total flavonoids from Lycopodii herba were studied.In order to increase the extraction yield of total flavonoids,the ethanol reflux extraction conditions of total flavonoids from Lycopodii herba was optimized by orthogonal test L9 (34) based on the single-factor tests.Meanwhile,the antioxidant activity of total flavonoids from Lycopodii herba was evaluated by means of the scavenging capacity of the hydroxyl free radical and the DPPH free radical.The results showed that the optimum extraction parameters were 75% of the volume fraction of ethanol,60∶1 mL/g of liquid material ratio,150 minutes of extraction time,80℃ of extraction temperature,and under these conditions,the average extraction rate of total flavonoids was 1.82%.When the concentration of total flavonoids was 0.014 0 mg/mL,the scavenging rates of total flavonoids on ?OH and DPPH? were 72.65% and 93.02%,respectively,which implied that the total flavonoids had good ability to scavenge ?OH and DPPH?.
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备注/Memo
第一作者简介:杨申明(1976-),男,云南双柏人,本科,实验师,现主要从事天然有机产物化学研究及教学工作。E-mail:ysm@cxtc. edu.cn.责任作者:王振吉(1983-),男,辽宁绥中人,博士,副教授,现主要从事天然产物化学等研究工作。E-mail:wangzj@cxtc.edu.cn.基金项目:国家自然科学基金资助项目(31300370);云南省重点建设学科资助项目(05YJJSXK03);楚雄师范学院重点建设学科基金资助项目(05YJJSXK03);云南省高校科技创新团队支持计划资助项目(IRTSTYN)。