LIU Qiao,ZHANG Lihua,WANG Yuejin,et al.Cytological Study of Embryo and Endosperm Abortion in Two Seedless Grape Varieties[J].Northern Horticulture,2016,40(03):31-35.[doi:10.11937/bfyy.201603009]
两个无核葡萄品种胚及胚乳败育的细胞学研究
- Title:
- Cytological Study of Embryo and Endosperm Abortion in Two Seedless Grape Varieties
- Keywords:
- seedless grapes; embryo abortive; endosperm abortive; paraffin section; embryo rescue; sampling period
- 文献标志码:
- A
- 摘要:
- 以2个引自美国的种子败育型无核葡萄品种“木星”、“海王星”为试材，采用石蜡切片技术和铁矾苏木精染色法，对这2个品种的胚、胚乳进行细胞学观察，以期了解其胚败育的时期,从而确定其用作胚挽救母本时的最佳取样时期。结果表明：花后38 d和36 d，“木星”、“海王星”的胚分别开始发生败育；“木星”、“海王星”用作胚挽救母本的最佳取样时期分别是花后38 d和36 d。
- Abstract:
- Stenospermocarpic seedless grape cultivars ‘Jupiter’ and ‘Neptune’ were taken as materials，using paraffin section technique and iron vitriol hematoxylin staining，cytological observation was performed on abortion and development of ovules，endosperms and embryos in 2 stenospermocarpic seedless grapes cultivars，so as to determine the best sampling time when the cultivars were used as the embryo rescue female parents.The result showed that the embryo of ‘Jupiter’ and ‘Neptune’ abortive began to occur when 38 days and 36 days after full bloom respectively.The optimal sampling time of ‘Jupiter’ and ‘Neptune’，which were as female parents of seedless grape embryo rescue were 38 days and 36 days after full bloom respectively.
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备注/Memo
第一作者简介：刘巧（1988-），女，硕士，研究方向为葡萄种质资源与生物技术。E-mail：330166912@qq.com.责任作者：张剑侠（1964-），男，教授，博士生导师，研究方向为葡萄种质资源与生物技术。E-mail：zhangjx666@126.com.基金项目：国家现代农业产业技术体系建设专项基金资助项目（CARS-30-yz-7）；西北农林科技大学唐仲英作物育种基金资助项目（2014-97）。