WU Xinyi,XU Pei,WU Xiaohua,et al.Estimation of Genome of Bottle Gourd[J].Northern Horticulture,2016,40(02):110-112.[doi:10.11937/bfyy.201602029]
瓠瓜基因组测定
- Title:
- Estimation of Genome of Bottle Gourd
- Keywords:
- bottle gourd; genome; flow cytometry; rice
- 文献标志码:
- A
- 摘要:
- 以4份来自中国不同地区的瓠瓜（Lagenaria siceraria (Molina) Standl.）纯系为试材，采用流式细胞术测定其基因组大小。结果表明：6种细胞核裂解液对瓠瓜嫩叶进行处理，发现Otto I最适合瓠瓜细胞裂解；从5种候选内标作物中筛选出水稻日本晴(Oryza sativa L．var．Nipponbare)为最适内标作物；4份中国瓠瓜种质资源的基因组大小为329.11~344.56 Mb。
- Abstract:
- Taking four Chinese bottle gourds from different areas as material，flow cytometry was used to determine the genome size of bottle gourd.The results showed that the treatment effect of 6 nuclei isolation buffers was compared and Otto I was found as the best buffer for bottle gourd；the rice variety Nipponbare was selected as the optimal internal standard from 5 candidate crops；the genome size of 4 Chinese bottle gourd germplasm was about 329.11-344.56 Mb.
参考文献/References:
[1]HEISER C B.The gourd book：A thorough and fascinating account of gourds from throughout the world[M].Norman：University of Oklahoma Press，1979. [2]ERICKSON D L，SMITH B D，CLARKE A C，et al.An asian origin for a 10 000-year-old domesticated plant in the Americas[J].Proc Natl Acad Sci，2005，102：18315-18320. [3]XU P，XU S Z，WU X H，et al.Population genomic analyses from low-coverage RAD-Seq data：a case study on the non-model cucurbit bottle gourd[J].The Plant Journal，2014，177:430-442. [4]YETISIR H，SARI N.Effect of different rootstock on plant growth，yield and quality of watermelon[J].Aust J Exp Agric，2003，43:1269-1274. [5]BEEVY S S，KURIACHAN P.Chromosome numbers of south Indian Cucurbitaceae and a note on the cytological evolution in the family[J].J Cytol Genet，1996，31:65-71. [6]DOLEEL J，BARTO J，VOGLMAYR H.Nuclear DNA content and genome size of trout and human[J].Cytometry A，2003，51：127-128. [7]DOLEEL J，GREILHUBER J，SUDA J.Flow cytometry with plants：an overview.In Flow Cytometry with Plant Cells[M].Weinheim:Wiley-VCH，2007：41-65. [8]DOLEEL J，GREILHUBER J，SUDA J.Estimation of nuclear DNA content in plants using flow cytometry[J].Nature Protocols，2007（2）:2233-2244. [9]GALBRAITH D W，HARKINS R K，MADDOX M J，et al.Rapid flow cytometry analysis of the cell cycle in intact plant tissues[J].Science，1983，220:1049-1051. [10]LOUREIRO J，RODRIGUEZ E，DOLEEL J，et al.Two new nuclear isolation buffers for plant DNA flow cytometry：a test with 37 species[J].Annals of Botany，2007，100：875-888. [11]ARUMUGANATHAN K，EARLE E D.Nuclear DNA content of some important plant species[J].Plant Molecular Biology Reporter，1991（3）：208-218. [12]YU J，HU S N，WANG J，et al.A draft sequence of the rice genome (Oryza sativa L.ssp.indica)[J].Science，2002，296：79-92. [13]ACHIGAN-DAKO E，FUCHS J，AHANCHEDE A，et al.Flow cytometric analysis in Lagenaria siceraria (Cucurbitaceae) indicates correlation of genome size with usage types and growing elevation[J].Plant Syst Evol，2008，276：9-19. [14]邓果特，刘清波，蒋建雄，等.五节芒基因组大小测定[J].植物遗传资源学报，2013，14(2)：339-341. [15]李祯，伊贤贵，顾宇，等.山樱花基因组大小的测定[J].南京林业大学学报（自然科学版），2014，38(S1)：17-19.
相似文献/References:
[1]张宏磊,朱杰华,胡珍珠,等.致病疫霉基因组序列微卫星的发掘与开发分析[J].北方园艺,2013,37(17):110.
ZHANG Hong-lei,ZHU Jie-hua,HU Zhen-zhu,et al.Detection and Analysis of Microsatellites in the Phytophthora infestans Genome[J].Northern Horticulture,2013,37(02):110.
[2]姜生秀,兰海燕.杏仁基因组DNA微量提取的改良方法[J].北方园艺,2012,36(20):105.
JIANG Sheng-xiu,LAN Hai-yan.The Improved Method of Apricot Genomic DNA Trace Extraction[J].Northern Horticulture,2012,36(02):105.
[3]王峰,丁云花,李成琼,等.植物异源附加系研究进展[J].北方园艺,2012,36(19):195.
WANG Feng,DING Yun-hua,LI Cheng-qiong,et al.Research Progress in Alien Plant Additional Lines[J].Northern Horticulture,2012,36(02):195.
[4]高兴旺,王贤磊,杨雅婷,等.甜瓜基因组SSR位点分布规律与多态性初探[J].北方园艺,2014,38(15):93.
GAO Xing-wang,WANG Xian-lei,YANG Ya-ting,et al.Distribution and Polymorphism Characteristics of SSR Loci in Melon Genome[J].Northern Horticulture,2014,38(02):93.
[5]王晓娥,姚方杰.真菌降解木质纤维素酶系的研究进展[J].北方园艺,2015,39(03):176.[doi:10.11937/bfyy.201503051]
WANG Xiao-e,YAO Fang-jie.Research Progress on Lignocellulose Degradation Enzyme System of Fungi[J].Northern Horticulture,2015,39(02):176.[doi:10.11937/bfyy.201503051]
[6]许瑞瑞,高明刚,刘春香.苹果基因组中转录因子的鉴定与分析[J].北方园艺,2018,42(09):42.[doi:10.11937/bfyy.20173398]
XU Ruirui,GAO Minggang,LIU Chunxiang.Identification and Analysis of Transcription Factors in Apple Genome[J].Northern Horticulture,2018,42(02):42.[doi:10.11937/bfyy.20173398]
[7]高耀辉,马斌,肖凤洁,等.CRISPR/Cas9系统在园林植物中的研究展望[J].北方园艺,2019,43(15):133.[doi:10.11937/bfyy.20183493]
GAO Yaohui,MA Bin,XIAO Fengjie,et al.Prospect of Research on CRISPR/Cas9 System in Garden Plants[J].Northern Horticulture,2019,43(02):133.[doi:10.11937/bfyy.20183493]
[8]李雪松,华蓉,孙达锋,等.基于花脸香蘑基因组的SSR分子标记开发[J].北方园艺,2023,(02):112.[doi:10.11937/bfyy.20222019]
LI Xuesong,HUA Rong,SUN Dafeng,et al.Development of SSR Molecular Markers Based on Whole Genome of Lepista sordida[J].Northern Horticulture,2023,(02):112.[doi:10.11937/bfyy.20222019]
[9]焦枥禾,王凤利,马银鹏,等.黑木耳分子生物学研究进展[J].北方园艺,2023,(17):124.[doi:10.11937/bfyy.20230365]
JIAO Lihe,WANG Fengli,MA Yinpeng,et al.Research Progress on Molecular Biology of Auricularia heimuer[J].Northern Horticulture,2023,(02):124.[doi:10.11937/bfyy.20230365]
[10]张春雨,李小宇,王晨,等.百合斑驳病毒靖宇分离物全基因组序列测定与进化分析[J].北方园艺,2023,(24):59.[doi:10.11937/bfyy.20232243]
ZHANG Chunyu,LI Xiaoyu,WANG Chen,et al.Sequencing and Analysis of the Genome of Lily mottle virus (LMoV) Jingyu Isolate[J].Northern Horticulture,2023,(02):59.[doi:10.11937/bfyy.20232243]
备注/Memo
第一作者简介：吴新义（1984-），男，河南南阳人，博士，助理研究员，研究方向为瓠瓜和豇豆基因组学及分子育种。E-mail：wuxinyi@mail.zaas.ac.cn. 责任作者：李国景（1966-），男，博士，研究员，研究方向为蔬菜育种与栽培技术。E-mail：ligj@mail.zaas.ac.cn. 基金项目：浙江省自然科学基金资助项目(LY14C150004)；浙江省农业新品种选育重大科技专项资助项目（2012C12903）。