[1]付瑞敏,邢文会,谷亚楠,等.苹果采后青霉病拮抗菌株的分离、鉴定和低能N+注入诱变[J].北方园艺,2015,39(21):116-120.[doi:10.11937/bfyy.201521030]
　FU Ruimin,XING Wenhui,GU Yanan,et al.Isolation and Identification of Antagonistic Bacteria Against Postharvest Blue Mold Decay of Apple and Its Mutation by Low Energy N+Implantation[J].Northern Horticulture,2015,39(21):116-120.[doi:10.11937/bfyy.201521030]

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苹果采后青霉病拮抗菌株的分离、鉴定和低能N+注入诱变

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷期数: 2015年21 页码: 116-120 栏目: 出版日期: 2015-11-15

Title:: Isolation and Identification of Antagonistic Bacteria Against Postharvest Blue Mold Decay of Apple and Its Mutation by Low Energy N+Implantation

作者:: 付瑞敏1; 2; 邢文会1; 谷亚楠2; 薛婷婷2; 郭彦钊2; 陈五岭2; （1.河南教育学院生命科学学院，河南郑州 450046；2.西北大学生命科学学院，陕西西安 710069）

Author(s):: FU Ruimin1; 2; XING Wenhui1; GU Yanan2; XUE Tingting2; GUO Yanzhao2; CHEN Wuling2; （1.Department of Life Science，Henan Institute of Education,Zhengzhou，Henan 450046；2.College of Life Science，Northwest University,Xi’an，Shaanxi 710069）

关键词:: 苹果; 采摘后; 青霉病; 解淀粉芽胞杆菌; 低能N+注入

Keywords:: apple; postharvest; blue mold decay; Bacillus amyloliquefaciens; low energy N+ implantation

DOI:: 10.11937/bfyy.201521030

文献标志码:: A

摘要:: 以苹果表面分离到拮抗扩展青霉的菌株BA-16为试材，经形态学、生理生化及16S rRNA序列分析，该菌被鉴定为解淀粉芽孢杆菌（Bacillus amyloliquefaciens）。为了增强该菌株防治青霉病的能力，通过低能N+注入技术对其进行诱变选育，获得抑菌性能显著提高且遗传性能稳定的突变株BA-16-8。为检测该突变株防治青霉病的增强效果，分别对野生株BA-16和突变株BA-16-8的菌悬液和无细胞发酵液进行果实生防检测。结果表明：突变株的菌体及代谢产物的抗病性能显著高于野生菌株，对于苹果采后青霉病的生物防治具有良好的应用开发前景。

Abstract:: Taking an antagonistic strain BA-16 which could inhibit Penicillium expansum isolated from apple surface as metarial，phenotypic，physiological，biochemical and phylogenetic (16SrRNA) charactors were studied.The strain BA-16 was identified as Bacillus amyloliquefaciens. In order to enhance its antagonistic capability，the mutation of BA-16 was carried out by using low energy N+ implantation.After the mutation，the mutant BA-16-8，which showed the strongest antagonist capability and stable hereditary stability was selected out.Aiming at estimating the increasing biological control effects of apple blue mold decay by mutant BA-16-8，the inhibition effects of bacterial broth and cell-free fermentation broth of mutant and wild-type strain were studied by biological control trial on fruit.The results showed that comparing with its wild type,the mutant displayed significantly higher effect on controlling apple blue mold disease.Therefore,the mutant BA-16-8 will be a potential biological control agent against apple blue mold decay.

参考文献/References:

[1]ZHAO L，ZHANG H，LIN H，et al.Effect of trehalose on the biocontrol efficacy of pichia caribbica against post-harvest grey mould and blue mould decay of apples[J].Pest Management Science,2013，69(8)：983-989. [2]LI R，ZHANG H，LIU W，et al.Biocontrol of postharvest gray and blue mold decay of apples with rhodotorula mucilaginosa and possible mechanisms of action[J].International Journal of Food Microbiology,2011，146(2)：151-156. [3]HONG P，HAO W，LUO J，et al.Combination of hot water，bacillus amyloliquefaciens hf-01 and sodium bicarbonate treatments to control postharvest decay of mandarin fruit[J].Postharvest Biol Tec，2014，88：96-102. [4]AFSHARMANESH H，AHMADZADEH M，JAVAN-NIKKHAH M,et al.Improvement in biocontrol activity of bacillus subtilis utb1 against aspergillus flavus using gamma-irradiation[J].Crop Prot，2014，60：83-92. [5]姚磊,徐良雄,薛璟花,等.枝顶孢属真菌的抑菌活性及其代谢产物研究[J].热带亚热带植物学报,2012，20(2):192-196. [6]刘雪，叶婧，穆长青,等.枯草芽孢杆菌 b-332 菌株发酵条件的研究[J].农业科学与技术（英文版），2013(1)：81-85. [7]韩立荣,张华姣,高保卫,等.放线菌 11-3-1 对油菜菌核病的防治作用与菌株鉴定[J].植物保护学报，2012(2)：97-102. [8]韩欣宇,陈志厚,罗定棋,等.Tpb55 菌株发酵液中活性代谢产物的抑菌作用及稳定性测定[J].中国农学通报,2012(27)：260-264. [9]韦婉,李少英,王梦姣,等.益生乳酸菌对氟喹诺酮类药物的敏感性检测方法的研究[J].食品科技，2013(3)：22-26. [10]黄文芳，张松.微生物学实验指导[M].广州：暨南大学出版社，2003. [11]布坎南,吉本斯.伯杰细菌鉴定手册[M].8版.北京：科学出版社，1984. [12]孙卓,杨利民.人参病原菌拮抗细菌的分离筛选与鉴定[J].植物保护学报，2015(1):79-86. [13]PARENICOVA L，SKOUBOE P，FRISVAD J,et al.Combined molecular and biochemical approach identifies aspergillus japonicus and aspergillus aculeatus as two species[J].Appl Environ Microb，2001，67(2)：521-527. [14]蒋益,郑惠华,刘广建,等.低能 N+离子注入诱变选育云芝高产抗病菌株的研究[J].育种驯化，2014(2)：18-20. [15]YANEZ-MENDIZABAL V，ZERIOUH H，VINAS I,et al.Biological control of peach brown rot (monilinia spp.) by bacillus subtilis cpa-8 is based on production of fengycin-like lipopeptides[J].Eur J Plant Pathol，2012，132：609-619. [16]张瑞,周俊,王舒雅,等.产γ-聚谷氨酸菌株的诱变选育及其种子液工艺优化[J].生物加工过程,2015，13(1)：47-53. [17]周小虹,闫冬,陆兆新,等.酵母菌 a82-2 的分类鉴定及对桃软腐病菌的抑菌效果研究[J].浙江大学学报(农业与生命科学版)，2013，39(1)：75-83. [18]朱兆香,王相晶.利用代谢工程的方法提高放线菌次级代谢物的产量[J].中国科技论文在线,2011(1)：1-13.

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备注/Memo

第一作者简介：付瑞敏（1981-），女，河南郑州人，博士研究生，讲师，现主要从事农业及食品微生物等研究工作。E-mail：angelaminmin@163.com.责任作者：陈五岭（1954-），男，陕西西安人，硕士，教授，博士生导师，现主要从事农业和环境及食品微生物等研究工作。E-mail：wuling.chen@263.net.基金项目：农业部科技成果与转化资助项目（2012GB2G000451）；陕西省重大科技创新资助项目(2009ZKC04-16)；河南教育学院青年科研课题资助项目（20100103）。

更新日期/Last Update: 2015-12-28