KANG Pingzhi,WU Xiuhong,SHEN Ruiqing,et al.Identification of the Pathogenic Fungus Causing Leaf Spot of Zizyphus jujuba in Ningxia and Fungicide Screening in Laboratory[J].Northern Horticulture,2015,39(18):122-124.[doi:10.11937/bfyy.201518032]
宁夏红枣叶斑病病原菌鉴定及室内药剂筛选
- Title:
- Identification of the Pathogenic Fungus Causing Leaf Spot of Zizyphus jujuba in Ningxia and Fungicide Screening in Laboratory
- Keywords:
- Zizyphus jujuba; leaf spot; identification; Stemphylium sp.; fungicide screening
- 文献标志码:
- A
- 摘要:
- 以宁夏红枣叶斑病典型病叶为试材，采用组织分离法、离体叶片菌块接种法和菌丝生长抑制法，研究了该地区红枣叶斑病的致病菌种类及有效防治药剂。结果表明：从宁夏红枣叶斑病典型病叶上主要分离得到匍柄霉属真菌（Stemphylium sp.），占总分离物的85.0％，其中匍柄霉菌株Y-14具有很强的致病性，是宁夏红枣叶斑病的主要病原菌种类；7种杀菌剂对病原菌的毒力强弱依次为25％丙环唑EC＞25％咪鲜胺EC＞1 000亿/g枯草芽孢杆菌WP＞10％多抗霉素WP＞250 g/L嘧菌酯SC＞70％代森锰锌WP＞1％申嗪霉素SC，尤其前5种杀菌剂的EC50值＜1.0 g/L，可作为宁夏红枣叶斑病生物防治和化学防治的首选药剂。
- Abstract:
- Taking typical leaves of leaf spot of Zizyphus jujuba in Ningxia as materials，the main pathogen species of leaf spot of Zizyphus jujuba in the region and effective fungicides against the disease were studied by the tissue isolation，detached leaves inoculation and inhibition method of mycelial growth.The results showed that Stemphylium sp.were isolated from typical leaves of leaf spot of Zizyphus jujuba in Ningxia and accounted for 85% of the pathogen total isolates.Among them，strain Y-14 of Stemphylium sp.had very strong pathogenicity and was confirmed as the main pathogen species of leaf spot of Zizyphus jujuba in Ningxia; Virulences of 7 kinds of fungicides on pathogen were 25% propiconazole EC>25% prochloraz EC>1010/g Bacillus subtilis WP>10% polyoxin WP>250 g/L azoxystrobin SC>70% mancozeb WP>1% phenazine-1-carboxylic acid SC，especially EC50 value of anterior 5 kinds of fungicides were less than 1.0 g/L，so they should be the best fungicides of leaf spot of Zizyphus jujuba in Ningxia for effective biological and chemical control.
参考文献/References:
[1]郝凤霞，杨敏丽，杨彦忠.宁夏红枣中总黄酮含量的比较[J].湖北农业科学，2011，50(6):1272-1274. [2]王志霞，孙洁，赵思峰，等.新疆矮化密植枣园红枣叶斑病病原鉴定[J].中国森林病虫，2013，32(4):1-5. [3]杨淑荣，王延丽，谢昌平，等.加那利海枣叶斑病病原鉴定[J].植物保护，2011，37(5):144-147. [4]方中达.植病研究方法[M].3版.北京:中国农业出版社，1998:24-151. [5]黄燕，段灿星，陆鸣，等.蚕豆赤斑病病原菌鉴定[J].植物保护，2012，38(6):115-118. [6]王志霞，王斌，赵思峰，等.新疆红枣叶斑病菌生物学特性及室内药剂筛选研究[J].北方园艺，2013(8):132-135. [7]冯福娟，佘德松.加拿利海枣叶斑病抑菌试验[J].浙江林业科技，2012，32(1):47-49. [8]蔺经，杨青松，李晓刚，等.嘧霉胺对梨黑斑病菌(Alternaria alternate)的毒力及其药效评价[J].植物保护，2009，35(4):162-163. [9]杨紫红，喻国辉，陈远凤，等.棕榈科植物叶斑病病原菌的生物学特性及药剂筛选试验[J].广东农业科学，2008(6):64-66，89. [10]薛峰.暗色丝孢真菌细基格孢属（Ulocladium）、匍柄霉属（Stemphylium）及链格孢属（Alternaria）的分类和分子系统学研究[D]．泰安:山东农业大学，2005. [11]张天宇.中国真菌志第三十一卷 暗色砖格分生孢子真菌26属（链格孢属除外)[M]．北京:科学出版社，2009.
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备注/Memo
第一作者简介：康萍芝（1972-），女，本科，研究员，现主要从事土壤微生物分离与鉴定和植物病害综合防治等研究工作。E-mail:kangpingzhi@163.com。责任作者：沈瑞清（1964-），男，博士，研究员，现主要从事真菌学分类及植物病害综合防治等研究工作。E-mail：srqzh@sina.com。基金项目：国家星火计划资助项目（2012GA880001）。