ZHOU Yin,WANG Songtai,DONG Kun,et al.Cloning and Expression Analysis of Allene Oxide Cyclase Gene (CfAOC) in Cymbidium faberi[J].Northern Horticulture,2015,39(14):84-89.[doi:10.11937/bfyy.201514023]
蕙兰CfAOC基因的克隆及表达分析
- Title:
- Cloning and Expression Analysis of Allene Oxide Cyclase Gene (CfAOC) in Cymbidium faberi
- Keywords:
- Cymbidium faberi; Allene oxide cyclase gene (CfAOC); jasmonate biosynthesis; expression analysis
- 文献标志码:
- A
- 摘要:
- 以野生蕙兰为试材，利用分子手段解析CfAOC（allene oxide cyclase，丙二烯氧化物环化酶）在蕙兰花香合成途径中的作用，同时为揭示蕙兰花香合成的分子机制提供理论依据。结果表明：分别以蕙兰基因组DNA和cDNA为模板PCR扩增出CfAOC基因的全长序列和CDS序列，比较发现CfAOC基因由3个外显子和2个内含子构成，亚细胞定位预测该蛋白位于叶绿体中，氨基酸序列分析显示该基因编码的蛋白含有保守的丙二烯氧化物环化酶结构域，进化树结果也表明CfAOC与其它植物的AOC同源性较高，都参与茉莉酸及其衍生物的合成代谢。CfAOC基因的时空特异性表达分析发现该基因在蕙兰盛花期时的花中表达量最高，暗示该基因可能与蕙兰花香合成有关。
- Abstract:
- Taking Cymbidium faberi as material,focus on one of the most crucial enzymes，CfAOC，which participates in the biosynthesis of methyl jasmonate，the main component of the flower fragrance in C.faberi.The paper was to provide theoretical basis for the identification of molecular mechanism in the flower fragrance biosynthesis in C.faberi.The results showed the full-length and CDS sequences of CfAOC were obtained using PCR amplification with gDNA and cDNA of C.faberi as templates，respectively.The sequencing results demonstrated that CfAOC had 3 exons truncated with 2 introns.CfAOC was predicted to be located in chloroplast by subcellular localization，with a conserved amino acid region of allene oxide cyclase domain.The phylogenetic tree proved that CfAOC was homologous to AOC in the other plants as well，involved in the jasmonates biosynthesis.The spatial temporal specific expression analysis revealed that higher transcript levels of CfAOC were in flower at blooming stage，which suggested that CfAOC could be related with the flower scent biosynthesis in C.faberi.
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备注/Memo
第一作者简介：周银（1983-），女，博士，讲师，研究方向为植物分子生物学。E-mail：ripplet0931@hotmail.com。基金项目：国家自然科学基金资助项目（31201611）。