ZHU Pengfang,WANG Weizhen,LI Jun,et al.Study on Microspore Embryogenesis，Regeneration and Propagation in vitro on Ornamental Kale (Brassica oleracea var.acephala)[J].Northern Horticulture,2015,39(13):111-115.[doi:10.11937/bfyy.201513032]
羽衣甘蓝游离小孢子胚胎发生、植株再生与增殖的研究
- Title:
- Study on Microspore Embryogenesis，Regeneration and Propagation in vitro on Ornamental Kale (Brassica oleracea var.acephala)
- 文章编号:
- 1001-0009（2015）13-0111-05
- Keywords:
- Brassica oleracea L.var. acephala DC.; microspore; embryogenesis; plant regeneration; propagation
- 分类号:
- S 635.9
- 文献标志码:
- A
- 摘要:
- 以20份羽衣甘蓝材料为试材，采用游离小孢子培养的方法，研究了胚胎发生、胚状体成苗和丛生芽继代培养的影响因素。结果表明：在诱导胚胎发生过程中，基因型是关键因素，供试材料中有15份诱导出胚，诱导率为75%；最适蔗糖浓度也与基因型有关；在不同基因型中，6-BA与NAA最佳浓度配比为1∶1或2∶1。与B5相比，MS培养基更有利于胚状体成苗。丛生芽转接周期为15 d时增殖效果最好，平均增殖系数为10.3。
- Abstract:
- Using twenty materials in ornamental kale (Brassica oleracea L.var. acephala DC.) as material,using microspore culture,influence factors of embryogenesis，plant regeneration and the subculture of multiple shoots were studied systematically.The results showed that the genotype was one of the most important factors to embryogenesis.Fifteen genotypes produced embryos with a seventy five percent induction ratio.The optimal sucrose concentration was also associated with genotypes.In different genotypes，the best ratio of 6-BA and NAA was 1∶1 or 2∶1.Compared with B5 medium，MS was better in plant regeneration.The best subculture duration shoots was 15 days with an average propagation coefficient of 10.3.
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备注/Memo
第一作者简介：祝朋芳（1971-），女，博士，教授，现主要从事观赏植物遗传育种与分子生物学等研究工作。E-mail:pfzhu2013@126.com.基金项目：国家自然科学基金资助项目（31101566）。