[1]张宏涛,陈纹,李小伟,等.低温胁迫下肋果沙棘试管苗黄酮类化合物合成关键酶的活性[J].北方园艺,2015,39(10):5-8.[doi:10.11937/bfyy.201510002]
　ZHANG Hong-tao,CHEN Wen,LI Xiao-wei,et al.The Activity of Key Enzymes Related to Flavonoids in Test-tube Plantlets of Hippophae neurocarpa Under Low Temperature[J].Northern Horticulture,2015,39(10):5-8.[doi:10.11937/bfyy.201510002]
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低温胁迫下肋果沙棘试管苗黄酮类化合物合成关键酶的活性

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年10 页码: 5-8 栏目: 出版日期: 2015-05-30
Title:
The Activity of Key Enzymes Related to Flavonoids in Test-tube Plantlets of Hippophae neurocarpa Under Low Temperature
文章编号:
1001-0009(2015)10-0005-04
作者:
张宏涛; 陈纹; 李小伟; 苏雪; 孙坤
（西北师范大学 生命科学学院，甘肃 兰州 730070)
Author(s):
ZHANG Hong-tao; CHEN Wen; LI Xiao-wei; SU Xue; SUN Kun
(College of Life Science，Northwest Normal University，Lanzhou，Gansu 730070)
关键词:
肋果沙棘(Hippophae neurocarpa); 试管苗; 低温胁迫; PAL; C4H; 4CL; 酶活性
Keywords:
Hippophae neurocarpa; test-tube plantlets; low-temperature stress; PAL; C4H; 4CL; enzyme activity
分类号:
S 793.6
DOI:
10.11937/bfyy.201510002
文献标志码:
A
摘要:
以青藏高原特有植物肋果沙棘试管苗为试材，研究了4℃低温胁迫(0～72 h)对其叶片黄酮类化合物合成关键酶苯丙氨酸解氨酶(PAL)、肉桂酸4-羟基化酶(C4H)及4-香豆酸辅酶A连接酶(4CL)活性的影响。结果表明：短期的低温胁迫显著影响肋果沙棘试管苗叶片PAL、C4H和4CL的活性。胁迫初期，PAL和C4H活性波动较大，PAL活性在12 h达到最高值，24 h时活性最低，比对照降低了63.514%，之后其活性逐渐恢复；C4H活性在处理12 h时降到最低，此后逐渐升高，72 h时其活性是对照的447.2%；4CL活性在胁迫6 h时达到最高值，比对照高出了36.285%，之后缓慢下降并最终低于对照。肋果沙棘试管苗叶片PAL、C4H和4CL以不同的方式对低温胁迫进行了响应，苯丙烷类代谢被激活，调控黄酮类等次生代谢物质的合成。
Abstract:
Test-tube plantlets leaves of Hippophae neurocarpa was used as materials，activities of three key enzymes related to flavonoids，phenylalanine ammonialyase (PAL)，4-hydroxy cinnamic acid enzyme (C4H) and 4-Coumadin acid coenzyme A ligase (4CL) were tested under low temperature in test-tube plantlets leaves of Hippophae neurocarpa.The results showed that the three enzymes could be affected easily under low temperature.In the early stage of stress，activities of PAL and C4H fluctuated greatly.The activity of PAL reached the highest value at 12 hours and got the lowest value by 24 hours，which was 63.514% lower than that of control.Its activity recovered gradually afterwards.The activity of C4H got the lowest value at 12 hours and began to increase after that.Its activity was 447.2% of the control by 72 hours.The activity of 4CL reached the highest after 6 hours and was 36.285% higher than that of control.The activity of 4CL decreased after that and was lower than that of control at last.The activities of PAL，C4H and 4CL in test-tube plantlets leaves of Hippophae neurocarpa changed in different ways under low temperature.Phenylaprapanoid metabolism was activated and synthesis of secondary metabolites such as flavonoids could be regulated.

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备注/Memo

第一作者简介：张宏涛（1986-），男，硕士研究生，研究方向为逆境植物进化与发育。

责任作者：陈纹(1978-)，女，硕士，高级实验师，研究方向为植物分子遗传学。E-mail：skychw@nwnu.edu.cn.
基金项目：国家自然科学基金资助项目（30160046,30960029）。

更新日期/Last Update: 2015-08-05