[1]赵兴宇,李丽丽,杨洪一.蓝莓茎段离体快繁研究[J].北方园艺,2015,39(01):103-105.[doi:10.11937/bfyy.201501028]
　ZHAO Xing-yu,LI Li-li,YANG Hong-yi.Study on Rapid Proliferation of Stem Segments in vitro of Blueberry[J].Northern Horticulture,2015,39(01):103-105.[doi:10.11937/bfyy.201501028]
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蓝莓茎段离体快繁研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年01 页码: 103-105 栏目: 出版日期: 2015-01-15
Title:
Study on Rapid Proliferation of Stem Segments in vitro of Blueberry
作者:
赵兴宇 1; 李丽丽 2; 3; 杨洪一 1
(1.东北林业大学 生命科学学院，黑龙江 哈尔滨 150040；2.黑龙江省林业科学研究所，黑龙江 哈尔滨 150081； 3.黑龙江省农业科学院 耕作栽培研究所，黑龙江 哈尔滨 150086)
Author(s):
ZHAO Xing-yu1; LI Li-li 2; 3; YANG Hong-yi 1
(1.College of Life Science,Northeast Forestry University,Harbin,Heilongjiang 150040；2.Institute of Forestry Science of Heilongjiang Province,Harbin,Heilongjiang 150081；3.Institute of Crop Tillage and Cultivation,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086)
关键词:
关键词：蓝莓; 外植体; 组织培养; 不定根
Keywords:
Keywords:blueberry; explants; tissue culture; adventitious root
DOI:
10.11937/bfyy.201501028
文献标志码:
A
摘要:
摘 要：以大兴安岭地区野生蓝莓苗木为试材，取当年生幼嫩茎段为外植体，探索建立蓝莓组培苗的快繁体系。结果表明：外植体消毒处理组合为75%乙醇处理10～15 s，0.1%升汞处理10 min。培养基为WPM培养基，愈伤组织诱导及不定芽增殖时期应用的激素组合ZT 2.0 mg/L+ NAA 0.2 mg/L。利用IBA蘸根法诱导不定根，激素浓度为200 μg/mL，芽诱导率超过80%，生根率超过70%。
Abstract:
Abstract:Taking the wild blueberry seedlings in Greater hinggan mountains area as test materials,using the young stem segments as explants,the blueberry somaclone of rapid propagation system was explored.The results showed the explants disinfection treatment combination was 75% ethanol for 10-15 seconds and 0.1% mercuric chloride for 10 min.The medium was WPM,and the hormones were ZT 2.0 mg/L+NAA 0.2 mg/mL in the steps of inducement of callus and proliferation of adventitious buds.Adventitious roots were induced with dipping method by 200 μg/mL IBA.The results showed that callus and adventitious buds could be formed from stem of blueberry by the medium and hormones,and the induced ratio of adventitious buds was higher than 80%.In addition,the rooting rate was more than 70%.

参考文献/References:

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备注/Memo

第一作者简介：赵兴宇（1989-），男，吉林人，硕士研究生，研究方向为微生物学。责任作者：杨洪一(1978-)，男，吉林九台人，博士，副教授，现主要从事微生物学等研究工作。E-mail:yhyi1@sohu.com.基金项目：国家自然科学基金资助项目(31200517;31300573)；中国博士后科学基金资助项目(2011M500706；2012T50382)。

更新日期/Last Update: 2015-02-16