ZHANG Hong-wei,TAN Wu-ping,LIANG Yi.Study on Transformation of Multi-visual Marker Gene in Onion[J].Northern Horticulture,2014,38(24):99-103.
洋葱可视化多重标记遗传转化研究
- Title:
- Study on Transformation of Multi-visual Marker Gene in Onion
- 文章编号:
- 1001-0009（2014）24-0099-05
- Keywords:
- onion; genetic transformation; anthocyanin
- 分类号:
- S 633.203.6
- 文献标志码:
- A
- 摘要:
- 以“凤凰白皮”洋葱种子为试材，采用转基因方法，将载体pBAC9075转入愈伤组织，研究花青素合成转录因子Bi和C1对转基因植株的影响。结果表明：经再生培养和草甘膦筛选，获得189棵再生植株，PCR检测发现5棵再生植株表现阳性，田间草甘膦筛选有7棵植株存活，其中2棵植株鳞茎有花青素积累，试验结果以期为花青素作为一种可视化跟踪系统用于洋葱转基因植株早期筛选提供依据。
- Abstract:
- Taking onion seeds as materials,using transgenic method,the vector pBAC9075 was transformed into the callus,and the effects to transgenic plants of anthocyanin synthesis transcription factor Bi and C1 were studied either. The result showed that,189 regenerated plants were obtained after the regeneration and glyphosate screening.PCR analysis demonstrated that 5 regenerated plants were successfully transformed.Only 7 of the 189 plants were survived after field glyphosate screening and 2 of them showed anthocyanin accumulation.The above results was to provided a basis for the visualization genetic marker anthocyanins on early screening of transgenic plants in onion.
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备注/Memo
第一作者简介：张洪伟（1986-），男，河北井陉人，研究实习员，研究方向为洋葱与胡萝卜遗传育种。E-mail：zhwwawzjcx@163.com.