LU Hai-ju,WANG Bo,PAN Liu-jun,et al.Screening on Conditions of Solid Fermentation for Biocontrol Strain P3.9 of Trichoderma atroviride [J].Northern Horticulture,2014,38(14):119-123.
深绿木霉P3.9生防菌株固体发酵条件优化筛选
- Title:
- Screening on Conditions of Solid Fermentation for Biocontrol Strain P3.9 of Trichoderma atroviride
- 文章编号:
- 1001-0009(2014)14-0119-05
- 分类号:
- S 476
- 文献标志码:
- A
- 摘要:
- 以分离自枇杷主干韧皮部的深绿木霉（Trichoderma atroviride）P3.9菌株为试材，以产孢量为指标，通过单因素试验和正交实验，研究固体发酵培养底物基质、碳源、氮源、磷酸盐及其浓度，含水量、接种量、温度和时间等培养条件组合，筛选P3.9菌株固体发酵最佳组合条件。结果表明：最佳底物基质为米糠，碳源为葡萄糖或D-果糖，氮源为磷酸二氢铵或硝酸钠，磷酸盐为磷酸氢二钠，温度为28℃，含水量为40%，接种量为9%；正交实验表明，其孢子生产的最佳培养基为以米糠为底物基质，在其中添加0.5%葡萄糖、0.5%D-果糖、0.5%磷酸二氢铵、0.05%磷酸氢二钠，其最佳培养条件为40%含水量、5%接种量、光暗交替28℃静止培养9 d；按优化后的培养条件培养，最高产孢量可达7.5×10.9孢子/g培养物质；此结论为其生防菌剂的固体发酵提供理论依据。
- Abstract:
- Taking Trichoderma atroviride isolate P3.9 isolated from trunk phloem of loquat as material,optimal substrates and its concentrations of medium,carbon source,nitrogen source,phosphate,water,inoclation,temperature and culture time were studied according to the mass conidial production by simple factor and orthogonal experiments,in order to screen out the best solid fermetation for biocontrol strain P3.9 of?Trichoderma atroviride.The results showed that the proper medium was rice bran，carbon source was glucose or D-fructose，nitrogen source was ammonium phosphate monobasic or dodium nitrate，phosphate was disodium hydrogen phosphate,water content was 40%,inoculation count was 9% and culture time was 28℃.The orthogonal experiment indicated that optimal medium of the highest yields of condia consisted 10 g rice bran,0.5% glucose,0.5% D-fructose，0.5% ammonium phosphate monobasic,0.05% disodium hydrogen phosphate.In addition，water content was 40%，inoculation count was 5%,culture temperature and time was respective 28℃ and 9 d （12 h light and 12 h dark）.By the optimal medium and culture conditions，the maximum conidial yield was 7.5×10.9 conidia/g dry material.This conclusion provided theoretical parameter for the industrial fermentation.
参考文献/References:
[ 1] Gary J S, Carmen S,Karina S, et aL Trichoderma theobromicola and T.paucisporum : two new species isolated from cacao in South America[J]. My cological Research , 2006 , 110(4) : 381-392.
[2]武汉琴，苏经迁，谢明英，等，茶树内生木霉种的鉴定及其在植物体内的定殖[J].菌物学报，2009，28(3)：342-348.
[3]纪丽莲．芦竹内生真菌F0238对烟草赤星病的防治作用[J].江苏农业科学，2005(2)：54-56．
[4]王国平，鲁书玲，郑必强，等，内生真菌紫杉木霉ZJUF0986菌株及其活性代谢产物防治水稻纹枯病的效果[J].中国生物防治，2009，25(1)：30-34．
[5] Bryan A B,Mary D S,Delilah W. Trichoderma speaes form endophyticassociations within Theobroma cacao trichomes[J]. Mycological Research,
2009 ,113 (12):1365-1376.
[6] Nagaraju A, Sudisha J, Mahadeva Murthy S,et aL Seed priming with Trichodema harzianum isolates enhances plant growth and induces resistance against PLasmopara haLstedii ,an incitant of sunflower downy mildew disease[J]. Australasian Plant Pathology, 2012, 41: 609-620.
[7]售海菊，全舒舟，李香香，等，枇杷内生真菌中拮抗石榴干腐病菌菌株的筛选[J]．热带农业科学，2013，33(7)：58-62．
[8]王英姿，祁之秋，魏松红，等．绿色木霉菌固体发酵培养基优化组合正交筛选[J]．植物保护，2007，33(2)：61-63．
[9]刘时轮，李勇，傅俊范，等．绿色木霉菌株Tv 04-2固体发酵条件研究[J]．华北农学报，2008，23(增刊)：244-247．
[10]王永东，蒋立科，岳永德，等，生防菌株哈茨木霉H-13固体发酵条件的研究[J].浙江大学学报，2006，32(6)：645-650.
[11]敖新宇，程立君，陈玉惠，生防木霉SS003菌株（Trichodermaatro uiride）的固体发酵工艺研究[J].江西农业大学学报，2012，34(6)：1256-1261．
[12]方中达，植病研究方法[M]．3版，北京：中国农业出版社，1998．
[13]刘连妹，屈海泳，牛潇，等．绿色木霉HT-OI的生物学特性和抑菌特性[J]．西北农业学报，2008，17(6)：179-183．
[14]高克祥，项存悌，骆会欣，等．木霉菌株T88生物学特性的研究[J]．东北林业大学学报，1995，23(2)：33-39．
[15]王斌，Ali Khatib Bakar，刘金亮，等，长枝木霉TICC鉴定及其生物学特性研究[J]．中国农学通报，2011，27(5)：338-345．
相似文献/References:
[1]熊仕俊,孙成龙,施闯,等.番茄青枯病菌拮抗放线菌的筛选及鉴定[J].北方园艺,2014,38(05):114.
XIONG Shi-jun,SUN Cheng-long,SHI Chuang,et al.Screening and Identifying of Antagonistic Actinomycetes Against Ralstonia solancearum[J].Northern Horticulture,2014,38(14):114.
[2]高 宇.茶尺蠖天敌生态学研究进展[J].北方园艺,2014,38(09):203.
GAO Yu.Research Progress on Ecology of Natural Enemies of Tea Geometrid[J].Northern Horticulture,2014,38(14):203.
[3]全桂静.苹果采后病害拮抗细菌的筛选与防腐效果研究[J].北方园艺,2013,37(11):130.
QUAN Gui-jing.Study on Screening of Antagonistic Bacteria for Apple Postharvest Diseases and Biocontrol Efficacy[J].Northern Horticulture,2013,37(14):130.
[4]欧善生,韦荣昌,吴庆华,等.广西凉粉草病虫害种类及防治方法研究[J].北方园艺,2013,37(01):177.
OU Shan-sheng,WEI Rong-chang,WU Qing-hua,et al.Study on Varieties of Diseases and Insect Pests and Control Measures of Mesona chinensis in Guangxi[J].Northern Horticulture,2013,37(14):177.
[5]穆瑞瑞.土壤微生物在农村植物病害生物防治中的作用分析[J].北方园艺,2013,37(08):194.
MU Rui-rui.Analysis of Soil Microorganism on Biological Control of Plant Diseases in Rural Areas[J].Northern Horticulture,2013,37(14):194.
[6]胡琼,张海松.木霉菌TC对辣椒疫病的防治效果[J].北方园艺,2012,36(03):147.
HU Qiong,ZHANG Hai-song.Effect of Biological Control of Pepper Phytophthora Blight by Trichoderma TC[J].Northern Horticulture,2012,36(14):147.
[7]葛平华,马桂珍,付泓润,等.油菜菌核病的生物防治研究进展[J].北方园艺,2013,37(02):185.
GE Ping-hua,MA Gui-zhen,FU Hong-run,et al.Research Progress of the Biological Control of Sclerotinia sclerotiorum[J].Northern Horticulture,2013,37(14):185.
[8]古丽吉米拉·米吉提,王志英,王 娜,等.木霉菌的生物防治分子机理[J].北方园艺,2013,37(23):206.
MIJITI Gulijimila,WANG Zhi-ying,WANG Na,et al.Molecular Mechanism of Biological Control of Trichoderma spp.[J].Northern Horticulture,2013,37(14):206.
[9]李 阳,宋素琴,王 静,等.苹果树腐烂病的防治研究进展[J].北方园艺,2015,39(13):194.[doi:10.11937/bfyy.201513054]
LI Yang,SONG Suqin,WANG Jing,et al.Research Progress on Control of the Valsa Cancer of Apple Trees[J].Northern Horticulture,2015,39(14):194.[doi:10.11937/bfyy.201513054]
[10]郑春寒,杨庆峰,肖艳,等.两种生物菌剂对安丘大姜姜瘟病的防效[J].北方园艺,2015,39(15):118.[doi:10.11937/bfyy.201515031]
ZHENG Chunhan,YANG Qingfeng,XIAO Yan,et al.Effect of Two Bio-control Agents Against Ginger Bacterial Wilt in Field[J].Northern Horticulture,2015,39(14):118.[doi:10.11937/bfyy.201515031]
备注/Memo
第一作者简介：鲁海菊(1978-)，女，云南大理人，博士研究生，副教授，现主要从事亚热带植物真菌分类和真菌病害等研究工作。E-mail:luhaiju2011@126.com.