LI Rong.Study on the Determination of Cercis chinensis Pollen Viability and Its Storage Method[J].Northern Horticulture,2014,38(11):116-119.
紫荆花粉生活力测定及贮藏方法研究
- Title:
- Study on the Determination of Cercis chinensis Pollen Viability and Its Storage Method
- 文章编号:
- 1001-0009(2014)11-0116-04
- Keywords:
- Cercis chinensi Bunge; pollen; germination; viability; pollen storage
- 分类号:
- Q 946. 83
- 文献标志码:
- A
- 摘要:
- 以紫荆花粉为试材，利用离体培养法、碘-碘化钾染色法（I2-KI）、醋酸洋红染色法和氯化三苯基四氮唑(TTC）染色法对花粉生活力进行测定，并研究了不同贮藏条件对花粉萌发的影响，以期了解紫荆花粉的萌发特性及适宜的贮藏方法。结果表明：培养基中添加不同浓度的蔗糖和硼酸对花粉的萌发有促进作用，紫荆花粉萌发的最适培养基为15%蔗糖+0.004%硼酸 +0.08%的琼脂，萌发率达88.46%；I2-KI染色法和TTC染色法不适用于测定紫荆花粉生活力；而醋酸洋红染色法可以作为测定紫荆花粉生活力的方法；花粉生活力随贮藏时间的延长而下降，最佳贮藏方法为4℃干燥，耐贮藏力超过26 d。
- Abstract:
- Taking fresh pollen of Cercis chinesis as material,pollen viability was tested by some methods including culture in vitro,I2-KI staining,carmine acetate dyeing and TTC,in order to study characteristics of pollen germination and the suitable method of pollen storage. The results showed that culture media with different concentration of sucrose and boric acid had good effect on the pollen germination.The pollen germination rate reached 8864% after cultured on the media supplemented with 15% sucrose,0.004% boric acid and 0.08% agar.I2-KI staining and TTC staining were not suitable for testing pollen viability of Cercis chinensis,carmine acetate dyeing could be used to test pollen viability of Cercis chinensis.Pollen vitality decreased as time gone on,4℃ dry storage was the best storage condition to keep the vitality of pollen.Pollen could be stored over 26 d with significant viability.
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备注/Memo
作者简介：李荣（1984-），女，本科，助理工程师，现主要从事园林工程等方面的研究工作。E-mail:ulegai@126.com.
基金项目：国家“十二五”科技支撑计划资助项目（2012BAD01B07）；
国家国际科技合作资助项目（2011DFA30490）。
收稿日期：2013-12-12