ZHANG Zhen-yu,WANG Peng,WANG Shu-an,et al.Study on Structure and Physiological Basis of Leaf Colouring in Lagerstroemia indica L.‘Jinwei’[J].Northern Horticulture,2014,38(04):68-72.
‘金薇’叶片呈色的结构和生理基础研究
- Title:
- Study on Structure and Physiological Basis of Leaf Colouring in Lagerstroemia indica L.‘Jinwei’
- 文章编号:
- 1001-0009(2014)04-0068-05
- 分类号:
- S 68
- 文献标志码:
- A
- 摘要:
- 以紫薇品种‘金薇’和‘金薇’野生型为试材，利用透射电镜观察比较了供试‘金薇’及其野生型叶肉细胞内叶绿体和线粒体的超微结构，测定了不同时期叶片中的叶绿素、类胡萝卜素和花色素苷的含量。结果表明：‘金薇’叶肉细胞内，叶绿体和线粒体结构均呈现异常，叶绿体内基粒和基粒片层较少，线粒体内部有空腔，嵴发育不完全；‘金薇’叶片内的叶绿素a、叶绿素b、类胡萝卜素和花色素苷等4种色素的含量均极显著低于野生型，总叶绿素含量减少的程度大于类胡萝卜素，总叶绿素/类胡萝卜素低至2.0左右，这是导致‘金薇’叶片呈现黄色的直接原因。
- Abstract:
- Taking the Lagerstroemia indicaL.‘Jinwei’ and its wild-type (CK) as materials，the leaf ultrastructure of chloroplasts and mitochondria were investigated by transmission electron microscopy，and the content of chlorophyll，carotenoids and anthocyanin were measured at different time.The results showed that the ultra structure of chloroplasts and mitochondria were abnormal in ‘Jinwei’ mesophyll cells.There was fewer stroma lamella and grana lamella in chloroplast.There was cavity and a small amount of crest in mitochondria.The content of chl a，chl b，carotenoids and anthocyanin in ‘Jinwei’ were significantly lower than those in CK.Reduction of the total chlorophyll content was greater than carotenoids，and the ratio of the total chlorophyll and carotenoid were significantly reduced to about 2.0，which was the immediate reason for the appearance of golden leaves in ‘Jinwei’.
参考文献/References:
[1]Yamasaki H，Takahashi S，Heshiki R.The tropical fig Ficus microcarpa L.f.cv.Golden Leaves lacks heat-stable dehydroascorbate reductase activity[J].Plant and Cell Physiology，1999，40(6)：640-646. [2]Takahashi S，Tamashiro A，Sakihama Y，et al.High-susceptibility of photosynthesis to photoinhibition in the tropical plant Ficus microcarpa L.f.cv.Golden Leaves[J].BMC Plant Biology，2002，2(1)：2. [3]唐前瑞，陈德富，陈友云，等.红檵木叶色变化的生理生化研究[J].林业科学，2006，42(2)：111-115. [4]朱书香，杨建民，王中华，等.4种李属彩叶植物色素含量与叶色参数的关系[J].西北植物学报，2009，29(8)：1663-1669. [5]许克福，黄成林，庄艳.洒金东瀛珊瑚光合生理特性的研究[J].安徽农业大学学报，2009，36(4)：618-622. [6]赵云，王茂林，李江，等.幼叶黄化油菜(Brassica napusL.)突变体 Cr3529 叶绿体超微结构观察[J].四川大学学报(自然科学版)，2003，40(5)：974-977. [7]Jarvis P，Chen L J，Li H，et al.An Arabidopsis mutant defective in the plastid general protein import apparatus[J].Science，1998，282(5386)：100-103. [8]林宏辉，何礼，晏婴才，等.叶绿素缺乏大麦突变体叶绿体结构功能及生化特性的研究[J].四川大学学报(自然科学版)，2001，38(6)：899-904. [9]Falbel T G，Meehl J B，Staehelin L A.Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis[J].Plant Physiology，1996，112(2)：821-832. [10]董遵，刘敬阳，马红梅，等.甘蓝型油菜黄化(苗)突变体的叶绿素含量及超微结构[J].中国油料作物学报，2000，22(3)：27-34. [11]吴殿星，舒庆尧，夏英武，等.水稻转绿型白化突变系W25的叶绿体超微结构研究[J].浙江大学学报(农业与生命科学版),1997,23(4):51-52. [12]常青山，陈发棣，滕年军，等.菊花黄绿叶突变体不同类型叶片的叶绿素含量和结构特征比较[J].西北植物学报，2008，28(9)：1772-1777. [13]吴建慧，崔艳桃，赵倩竹.干旱胁迫下4种委陵菜属植物叶片的超微结构[J].草业科学，2012，29(11)：1724-1730. [14]陈延惠，李跃霞，郭晓丽，等.园艺植物叶色变化机制研究进展[J].河南农业科学，2011，40(12)：30-34. [15]Schelbert S，Aubry S，Burla B，et al.Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown during leaf senescence in Arabidopsis[J].The Plant Cell Online，2009，21(3)：767-785. [16]王平荣，张帆涛，高家旭，等.高等植物叶绿素生物合成的研究进展[J].西北植物学报，2009，29(3)：629-636. [17]陶俊，张上隆.园艺植物类胡萝卜素的代谢及其调节[J].浙江大学学报（农业与生命科学版），2003，29(5)：585-590. [18]Toledo-Ortiz G，Huq E，Rodríguez-Concepción M.Direct regulation of phytoene synthase gene expression and carotenoid biosynthesis by phytochrome-interacting factors[J].Proceedings of the National Academy of Sciences，2010，107(25)：11626-11631. [19]姜卫兵，庄猛，韩浩章，等.彩叶植物呈色机理及光合特性研究进展[J].园艺学报，2005，32(2)：352-358. [20]Pourcel L，Irani N G，Lu Y，et al.The formation of anthocyanic vacuolar inclusions in Arabidopsis thaliana and implications for the sequestration of anthocyanin pigments[J].Molecular Plant，2010，3(1)：78-90. [21]郑健，胡增辉，郭守华，等.金叶女贞的光合色素特性及光合性能[J].经济林研究，2012，30(1)：13-18. [22]钟传飞，张运涛，姚洪军，等.金叶女贞遮阴复绿过程中光系统 Ⅱ 功能转变研究[J].北京林业大学学报，2011，33(6)：35-39. [23]潘一铭.遮荫对金叶女贞和红檵木叶色影响的研究[D].成都：四川农业大学，2011. [24]代波.金叶榆，家榆光合特性及抗寒性的比较研究[D].保定：河北农业大学，2009. [25]郁慧，刘中亮，胡宏亮，等.干旱胁迫对5种植物叶绿体和线粒体超微结构的影响[J].植物研究，2011，31(2)：152-158. [26]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:134-138. [27]Rabino I，Mancinelli A L.Light，temperature，and anthocyanin production[J].Plant Physiology，1986，81(3)：922-924. [28]肖华贵，杨焕文，饶勇，等.甘蓝型油菜黄化突变体的叶绿体超微结构，气孔特征参数及光合特性[J].中国农业科学，2013，46(4)：715-727. [29]吴跃进，王学栋，吴敬德，等.水稻温敏型叶绿素突变体遗传及超微结构研究[J].安徽农业大学学报，1991(4)：3. [30]贾玉峰，许耀奎，邬信康.春小麦黄绿色突变系的遗传及叶绿体结构的分析[J].吉林农业大学学报，1992，14(1)：1-5. [31]肖华贵，杨焕文，饶勇，等.甘蓝型油菜黄化突变体的光合特性及叶绿素荧光参数分析[J].作物学报，2013，39(3)：520-529. [32]胡海姿，张睿，尚爱芹，等.金叶植物色素含量对光强的响应[J].园艺学报，2007，34(3)：717-722.
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备注/Memo
第一作者简介：张振宇（1991-），男，河南新乡人，硕士研究生，现主要从事园林植物等研究工作。E-mail:zzy_199101@139.com.